{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,19]],"date-time":"2026-04-19T05:02:53Z","timestamp":1776574973466,"version":"3.51.2"},"reference-count":58,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2018,8,21]],"date-time":"2018-08-21T00:00:00Z","timestamp":1534809600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100009457","name":"Region Sj\u00e6lland","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100009457","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clinical Exper Ophthalmology"],"published-print":{"date-parts":[[2019,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Importance<\/jats:title><jats:p>Geographic atrophy (GA) is a progressing atrophy of the neuroretina with no treatment option.<\/jats:p><\/jats:sec><jats:sec><jats:title>Background<\/jats:title><jats:p>Age\u2010related malfunction of retinal microglia amplifies response towards age\u2010related tissue stress in age\u2010related macular degeneration. Here, we investigated monocyte CD200 expression \u2013 the circulating middleman negotiating retinal microglial activity \u2013 in a poorly understood subtype of age\u2010related macular degeneration.<\/jats:p><\/jats:sec><jats:sec><jats:title>Design<\/jats:title><jats:p>Prospective case\u2013control study.<\/jats:p><\/jats:sec><jats:sec><jats:title>Participants<\/jats:title><jats:p>Forty\u2010six patients with GA and 26 healthy controls were included.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>All participants were subjected to a structured interview and detailed retinal examination. Controls were recruited from patient's spouses accompanying them in the clinic to match the groups best possibly. Participants had no history of immune disorders or cancer, and did not receive any immune\u2010modulating medication. Patients did not have any history or sign of choroidal neovascularization in either eye. Fresh drawn blood was stained with monoclonal antibodies and prepared for flow cytometry to evaluate CD200 expression in monocytes and their functional subsets.<\/jats:p><\/jats:sec><jats:sec><jats:title>Main Outcome Measures<\/jats:title><jats:p>The percentage of CD200+ monocytes in patients and controls.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>We found that monocytes were more CD200 positive in patients with GA compared to healthy age\u2010matched controls. Then, we explored the potential relationship between CD200 expression and important fundus autofluorescence patterns that predict disease progression. Patients with a high risk of progression (patients with high degree of hyperautofluorescence) had distinctly increased CD200 expression compared to other patients with GA.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions and Relevance<\/jats:title><jats:p>Our data reveals that abnormal monocytic CD200 expression is present in GA, and in particular among those identified as fast progressors.<\/jats:p><\/jats:sec>","DOI":"10.1111\/ceo.13362","type":"journal-article","created":{"date-parts":[[2018,7,26]],"date-time":"2018-07-26T02:55:53Z","timestamp":1532573753000},"page":"69-78","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":20,"title":["Patients with a fast progression profile in geographic atrophy have increased CD200 expression on circulating monocytes"],"prefix":"10.1111","volume":"47","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3804-7296","authenticated-orcid":false,"given":"Marie","family":"Krogh Nielsen","sequence":"first","affiliation":[{"name":"Clinical Eye Research Division, Department of Ophthalmology Zealand University Hospital  Roskilde Denmark"},{"name":"Faculty of Health and Medical Science University of Copenhagen  Copenhagen Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6620-5365","authenticated-orcid":false,"given":"Yousif","family":"Subhi","sequence":"additional","affiliation":[{"name":"Clinical Eye Research Division, Department of Ophthalmology Zealand University Hospital  Roskilde Denmark"},{"name":"Faculty of Health and Medical Science University of Copenhagen  Copenhagen Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christopher R","family":"Molbech","sequence":"additional","affiliation":[{"name":"Clinical Eye Research Division, Department of Ophthalmology Zealand University Hospital  Roskilde Denmark"},{"name":"Faculty of Health and Medical Science University of Copenhagen  Copenhagen Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mads K","family":"Falk","sequence":"additional","affiliation":[{"name":"Clinical Eye Research Division, Department of Ophthalmology Zealand University Hospital  Roskilde Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Amardeep","family":"Singh","sequence":"additional","affiliation":[{"name":"Clinical Eye Research Division, Department of Ophthalmology Zealand University Hospital  Roskilde Denmark"},{"name":"Department of Clinical Sciences Lund, Ophthalmology Skane University Hospital, Lund University  Lund Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mogens H","family":"Nissen","sequence":"additional","affiliation":[{"name":"Faculty of Health and Medical Science University of Copenhagen  Copenhagen Denmark"},{"name":"Eye Research Unit, Department of Immunology and Microbiology University of Copenhagen  Copenhagen Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Torben L","family":"S\u00f8rensen","sequence":"additional","affiliation":[{"name":"Clinical Eye Research Division, Department of Ophthalmology Zealand University Hospital  Roskilde Denmark"},{"name":"Faculty of Health and Medical Science University of Copenhagen  Copenhagen Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2018,8,21]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S2214-109X(13)70145-1"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(12)60282-7"},{"key":"e_1_2_5_4_1","first-page":"718","article-title":"In vivo fluorescence of the ocular fundus exhibits retinal pigment epithelium lipofuscin charateristics","volume":"36","author":"Delori FC","year":"1995","journal-title":"Invest Ophthalmol Vis Sci"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0891-5849(02)00959-0"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.05-0892"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajo.2006.11.041"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ophtha.2012.10.007"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1001\/jamaophthalmol.2013.5799"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-genom-090413-025610"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1350-9462(01)00010-6"},{"key":"e_1_2_5_12_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMra0801537"},{"key":"e_1_2_5_13_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12979-017-0100-9"},{"key":"e_1_2_5_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-017-00741-4"},{"key":"e_1_2_5_15_1","doi-asserted-by":"publisher","DOI":"10.18632\/aging.101329"},{"key":"e_1_2_5_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2008.06.080"},{"key":"e_1_2_5_17_1","doi-asserted-by":"publisher","DOI":"10.1111\/apm.12511"},{"key":"e_1_2_5_18_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.21538"},{"key":"e_1_2_5_19_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1474-9726.2007.00351.x"},{"key":"e_1_2_5_20_1","doi-asserted-by":"publisher","DOI":"10.1186\/2047-9158-1-9"},{"key":"e_1_2_5_21_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI31692"},{"key":"e_1_2_5_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neurobiolaging.2011.03.010"},{"key":"e_1_2_5_23_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2172-11-30"},{"key":"e_1_2_5_24_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12035-016-0245-0"},{"key":"e_1_2_5_25_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-physiol-022516-034406"},{"key":"e_1_2_5_26_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-017-08702-7"},{"key":"e_1_2_5_27_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1750-3639.2007.00098.x"},{"key":"e_1_2_5_28_1","doi-asserted-by":"publisher","DOI":"10.1159\/000252803"},{"key":"e_1_2_5_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1074-7613(00)00023-6"},{"key":"e_1_2_5_30_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.290.5497.1768"},{"key":"e_1_2_5_31_1","doi-asserted-by":"publisher","DOI":"10.1002\/glia.22366"},{"key":"e_1_2_5_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9440(10)64444-6"},{"key":"e_1_2_5_33_1","doi-asserted-by":"publisher","DOI":"10.1111\/jnc.13972"},{"key":"e_1_2_5_34_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2011-01-326827"},{"key":"e_1_2_5_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2010.08.012"},{"key":"e_1_2_5_36_1","first-page":"1588","article-title":"Complement gene expression and regulation in mouse retina and retinal pigment epithelium\/choroid","volume":"17","author":"Luo C","year":"2011","journal-title":"Mol Vis"},{"key":"e_1_2_5_37_1","doi-asserted-by":"publisher","DOI":"10.1080\/02713680490503778"},{"key":"e_1_2_5_38_1","doi-asserted-by":"publisher","DOI":"10.2174\/1389450043345164"},{"key":"e_1_2_5_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ophtha.2012.11.002"},{"key":"e_1_2_5_40_1","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.17-22479"},{"key":"e_1_2_5_41_1","doi-asserted-by":"publisher","DOI":"10.1093\/clinchem\/47.1.28"},{"key":"e_1_2_5_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1470-2045(08)70154-2"},{"key":"e_1_2_5_43_1","first-page":"15","article-title":"In patients with neovascular age\u2010related macular degeneration, physical activity may influence C\u2010reactive protein levels","volume":"8","author":"Subhi Y","year":"2014","journal-title":"Clin Ophthalmol"},{"key":"e_1_2_5_44_1","first-page":"A5303","article-title":"Physical activity patterns in patients with early and late age\u2010related macular degeneration","volume":"63","author":"Subhi Y","year":"2016","journal-title":"Dan Med J"},{"key":"e_1_2_5_45_1","volume-title":"Cochrane Handbook for Systematic Reviews of Interventions Version 5.1.0","author":"Higgins JPT","year":"2011"},{"key":"e_1_2_5_46_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00417-011-1846-y"},{"key":"e_1_2_5_47_1","doi-asserted-by":"publisher","DOI":"10.1002\/glia.20468"},{"key":"e_1_2_5_48_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.psyneuen.2008.02.013"},{"key":"e_1_2_5_49_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1474-9726.2010.00660.x"},{"key":"e_1_2_5_50_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-4835(02)00332-9"},{"key":"e_1_2_5_51_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbi.2009.05.060"},{"key":"e_1_2_5_52_1","doi-asserted-by":"publisher","DOI":"10.1186\/1742-2094-8-154"},{"key":"e_1_2_5_53_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbi.2011.10.004"},{"key":"e_1_2_5_54_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neurobiolaging.2012.06.010"},{"key":"e_1_2_5_55_1","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.10-7119"},{"key":"e_1_2_5_56_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2017.01.006"},{"key":"e_1_2_5_57_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2249.2009.04070.x"},{"key":"e_1_2_5_58_1","first-page":"210","article-title":"Complement C5a receptor knockout has diminished light\u2010induced microglia\/macrophage retinal migration","volume":"23","author":"Song D","year":"2017","journal-title":"Mol Vis"},{"key":"e_1_2_5_59_1","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.12-10069"}],"container-title":["Clinical &amp; Experimental Ophthalmology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fceo.13362","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/ceo.13362","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,14]],"date-time":"2023-09-14T08:27:52Z","timestamp":1694680072000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/ceo.13362"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,8,21]]},"references-count":58,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2019,1]]}},"alternative-id":["10.1111\/ceo.13362"],"URL":"https:\/\/doi.org\/10.1111\/ceo.13362","archive":["Portico"],"relation":{},"ISSN":["1442-6404","1442-9071"],"issn-type":[{"value":"1442-6404","type":"print"},{"value":"1442-9071","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,8,21]]},"assertion":[{"value":"2018-05-16","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-07-13","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-08-21","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}