{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,26]],"date-time":"2026-03-26T22:06:42Z","timestamp":1774562802278,"version":"3.50.1"},"reference-count":21,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2018,12,3]],"date-time":"2018-12-03T00:00:00Z","timestamp":1543795200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81472616"],"award-info":[{"award-number":["81472616"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81470623"],"award-info":[{"award-number":["81470623"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81770922"],"award-info":[{"award-number":["81770922"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81870661"],"award-info":[{"award-number":["81870661"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clinical Exper Ophthalmology"],"published-print":{"date-parts":[[2019,5]]},"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p>To compare the detection results consistency of quantitative polymerase chain reaction (qPCR) and digital droplet polymerase chain reaction (ddPCR), and determine the value of ddPCR for viral detection in the aqueous humour.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>A total of 130 aqueous humour samples were collected, including 60 patients with Posner\u2010Schlossman syndrome (PSS) in case group and 70 elderly patients with senile cataract in control group. The target nucleic acid fragments of human cytomegalovirus (HCMV), herpes simplex virus, Epstein\u2010Barr virus and varicella zoster virus in aqueous humour were analysed by qPCR and ddPCR, respectively, for the diagnosis and curative effect monitoring of pathogen\u2010induced PSS. Samples with inconsistent results were verified by next\u2010generation sequencing.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>There were 27 and 20 HCMV\u2010positive cases detected in the case group by ddPCR and qPCR, respectively. ddPCR increased the sensitivity for the HCMV virus detection from 400 to 100 copies\/mL. No other pathogens were found in this study. The results of ddPCR were consistent with that of next generation sequencing. The mean (SD) of Lg (HCMV copies\/mL) detected by ddPCR and qPCR were 1.66 (1.92) and 1.10 (1.61), respectively (<jats:italic>P<\/jats:italic> &lt; 0.001). Compared with qPCR, results of ddPCR showed better consistency with validity of clinical treatment. All patients with ddPCR\u2010positive results had good validity on antiviral therapy, exhibiting anterior chamber inflammation remission, resolution of corneal oedema and good IOP control within 1 month.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>HCMV was the leading cause of pathogen\u2010induced PSS in the Chinese population. ddPCR was a promising tool for early detection, accurate diagnosis and therapeutic validity monitoring of pathogen\u2010induced PSS. The high sensitivity of ddPCR could avoid repeated anterior chamber tap.<\/jats:p><\/jats:sec>","DOI":"10.1111\/ceo.13440","type":"journal-article","created":{"date-parts":[[2018,11,10]],"date-time":"2018-11-10T07:13:41Z","timestamp":1541834021000},"page":"513-520","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":28,"title":["Digital droplet polymerase chain reaction analysis of common viruses in the aqueous humour of patients with Posner\u2010Schlossman syndrome in Chinese population"],"prefix":"10.1111","volume":"47","author":[{"given":"Guojun","family":"Cao","sequence":"first","affiliation":[{"name":"Department of Laboratory Medicine, Huashan Hospital, Shanghai Medical College Fudan University  Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chen","family":"Tan","sequence":"additional","affiliation":[{"name":"Eye Institute, Eye and ENT Hospital, College of Medicine Fudan University  Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuyan","family":"Zhang","sequence":"additional","affiliation":[{"name":"Ophthalmology Department, Huashan Hospital, Shanghai Medical College Fudan University  Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8142-6040","authenticated-orcid":false,"given":"Xiangmei","family":"Kong","sequence":"additional","affiliation":[{"name":"Eye Institute, Eye and ENT Hospital, College of Medicine Fudan University  Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4440-1389","authenticated-orcid":false,"given":"Xinhuai","family":"Sun","sequence":"additional","affiliation":[{"name":"Eye Institute, Eye and ENT Hospital, College of Medicine Fudan University  Shanghai China"},{"name":"State Key Laboratory of Medical Neurobiology, Institutes of Brain Science and Collaborative Innovation Center for Brain Science, Shanghai Medical College Fudan University  Shanghai China"},{"name":"Shanghai Key Laboratory of Visual Impairment and Restoration  Shanghai China"},{"name":"Key Laboratory of Myopia, Ministry of Health  Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yanchun","family":"Ma","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, Huashan Hospital North, Shanghai Medical College Fudan University  Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Junyi","family":"Chen","sequence":"additional","affiliation":[{"name":"Eye Institute, Eye and ENT Hospital, College of Medicine Fudan University  Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8796-2653","authenticated-orcid":false,"given":"Ming","family":"Guan","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, Huashan Hospital, Shanghai Medical College Fudan University  Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2018,12,3]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.3109\/08820538.2011.605821"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/0002-9394(73)90818-0"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0161-6420(01)00551-6"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00136737"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12886-016-0369-z"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1097\/IIO.0b013e31822d6ab4"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jfo.2010.10.008"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01287-11"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1373\/clinchem.2008.112797"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.02136-14"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1442-9071.2012.02888.x"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.diagmicrobio.2017.08.002"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1007\/s13365-015-0390-7"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0110240"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.3988\/jcn.2016.12.4.446"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.4103\/0366-6999.154281"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajo.2012.12.008"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.5935\/0004-2749.20150094"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1080\/09273948.2017.1411952"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0044453"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1076\/ocii.10.4.263.15592"}],"container-title":["Clinical &amp; Experimental Ophthalmology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fceo.13440","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/ceo.13440","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/ceo.13440","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/ceo.13440","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,10]],"date-time":"2023-09-10T17:51:28Z","timestamp":1694368288000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/ceo.13440"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,12,3]]},"references-count":21,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2019,5]]}},"alternative-id":["10.1111\/ceo.13440"],"URL":"https:\/\/doi.org\/10.1111\/ceo.13440","archive":["Portico"],"relation":{},"ISSN":["1442-6404","1442-9071"],"issn-type":[{"value":"1442-6404","type":"print"},{"value":"1442-9071","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,12,3]]},"assertion":[{"value":"2018-09-12","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-11-03","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-12-03","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}