{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,30]],"date-time":"2026-06-30T03:59:40Z","timestamp":1782791980490,"version":"3.54.5"},"reference-count":13,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2019,5,27]],"date-time":"2019-05-27T00:00:00Z","timestamp":1558915200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clinical Exper Ophthalmology"],"published-print":{"date-parts":[[2019,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Importance<\/jats:title><jats:p>To illustrate the importance of lymphatic drainage in assessing trabeculectomy bleb function using intracameral trypan blue.<\/jats:p><\/jats:sec><jats:sec><jats:title>Background<\/jats:title><jats:p>To study the lymphatic drainage of trabeculectomy blebs using trypan blue, correlate with bleb function and classify them accordingly.<\/jats:p><\/jats:sec><jats:sec><jats:title>Design<\/jats:title><jats:p>Prospective cross\u2010sectional study in a tertiary care centre.<\/jats:p><\/jats:sec><jats:sec><jats:title>Participants<\/jats:title><jats:p>Thirteen glaucoma patients post\u2010trabeculectomy were studied.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Trypan blue was injected into the anterior chamber.<\/jats:p><\/jats:sec><jats:sec><jats:title>Main Outcome Measures<\/jats:title><jats:p>The duration taken for dye to stain a drainage bleb or lymphatics is recorded. The extent of the lymphatic structures were measured in clock hours. Intraocular pressure (IOP) prior to surgery was recorded.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Eight post\u2010trabeculectomy subjects with dye stained lymphatic vessels had lower IOP (12.6 mmHg, <jats:italic>P<\/jats:italic> =\u2009.013) compared to the five with no lymphatic vessel staining (mean IOP 23.6 mmHg). Lymphatic extent was inversely related to IOP (<jats:italic>P<\/jats:italic> =\u2009.021).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions and Relevance<\/jats:title><jats:p>Eyes with lymphatic connections to drainage blebs had lower IOP and reduced requirement for topical medications. The extent of lymphatic connection to drainage blebs is related to lower IOP.<\/jats:p><\/jats:sec>","DOI":"10.1111\/ceo.13534","type":"journal-article","created":{"date-parts":[[2019,5,10]],"date-time":"2019-05-10T12:17:47Z","timestamp":1557490667000},"page":"892-897","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["Use of trypan blue to assess lymphatic function following trabeculectomy"],"prefix":"10.1111","volume":"47","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0417-6201","authenticated-orcid":false,"given":"Ying J.","family":"Khoo","sequence":"first","affiliation":[{"name":"Lions Eye Institute University of Western Australia  Nedlands Western Australia Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aulia A. H.","family":"Abdullah","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, Faculty of Medicine Brawijaya University Dr Saiful Anwar General Hospital  Malang Indonesia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9490-534X","authenticated-orcid":false,"given":"Dao\u2010Yi","family":"Yu","sequence":"additional","affiliation":[{"name":"Lions Eye Institute University of Western Australia  Nedlands Western Australia Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3722-6922","authenticated-orcid":false,"given":"William H.","family":"Morgan","sequence":"additional","affiliation":[{"name":"Lions Eye Institute University of Western Australia  Nedlands Western Australia Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2019,5,27]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0039-6257(98)00025-3"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ophtha.2009.06.014"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1097\/IJG.0000000000000636"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.preteyeres.2009.06.004"},{"issue":"1","key":"e_1_2_6_6_1","first-page":"45","article-title":"Demonstration of aqueous outflow patterns of normal and glaucomatous human eyes through the injection of fluorescein solution in the anterior chamber (author's transl)","volume":"199","author":"Benedikt O","year":"1976","journal-title":"Ophthalmology"},{"key":"e_1_2_6_7_1","article-title":"Les vaisseaux lymphatiques de la conjonctive bulbaire humaine \u00e9tudi\u00e9s par la m\u00e9thode des injections vitales de bleutripan","volume":"8","author":"Busacca A","year":"1948","journal-title":"Arch Ophtalmol Revue G\u00e9n Ophtalmol"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1001\/jamaophthalmol.2014.3944"},{"key":"e_1_2_6_9_1","first-page":"79","article-title":"The use of trypan blue as a tracer to outline aqueous flow","volume":"27","author":"Morgan W","year":"2005","journal-title":"J Ophthalmic Photogr"},{"key":"e_1_2_6_10_1","volume-title":"R: A Language and Environment for Statistical Computing","author":"Team RC","year":"2013"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ophtha.2005.05.021"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1089\/lrb.2008.1008"},{"issue":"2","key":"e_1_2_6_13_1","first-page":"66","article-title":"Ocular lymphatics: state\u2010of\u2010the\u2010art review","volume":"42","author":"Chen L","year":"2009","journal-title":"Lymphology"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1159\/000068942"}],"container-title":["Clinical &amp; Experimental Ophthalmology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fceo.13534","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/ceo.13534","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/ceo.13534","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/ceo.13534","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,8]],"date-time":"2023-09-08T20:33:41Z","timestamp":1694205221000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/ceo.13534"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,5,27]]},"references-count":13,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2019,9]]}},"alternative-id":["10.1111\/ceo.13534"],"URL":"https:\/\/doi.org\/10.1111\/ceo.13534","archive":["Portico"],"relation":{},"ISSN":["1442-6404","1442-9071"],"issn-type":[{"value":"1442-6404","type":"print"},{"value":"1442-9071","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,5,27]]},"assertion":[{"value":"2018-12-22","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-04-23","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-05-27","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}