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How this secretome changes in diabetic eyes treated with anti\u2010vascular endothelial growth factor (VEGF) inhibitors is unclear.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Diabetic conditions were simulated in vitro using ARPE\u201019 cell\u2010line culture, with high glucose (25\u2009mM) culture media, and hypoxia was chemically induced using cobalt chloride. Stress was assessed using cell viability assays as well as Western blots and enzyme\u2010linked immunosorbent assay (ELISA) for production of HIF\u20101a and VEGF\u2010A. Production of neurotrophic factors was quantified once conditions were established using ELISA under stress with and without the addition of VEGF inhibitors. Changes were analysed with one\u2010way ANOVA.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Hypoxia downregulated pigment epithelium\u2010derived factor (PEDF) expression. The addition of bevacizumab, ranibizumab and aflibercept in normoxic conditions all led to a significant downregulation of PEDF. Glucose concentration had no effect on secretion of PEDF. Brain\u2010derived neurotrophic factor (BDNF) secretion was downregulated in high glucose and was upregulated in hypoxia. Placental growth factor (PlGF) secretion by ARPE\u201019 was undetectable by ELISA.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>We found that hypoxia, high glucose or VEGF inhibitors affected secretion of neurotrophic factors. This variation under different conditions may influence neuron and photoreceptor survival in the diabetic state and VEGF inhibitor treated eyes.<\/jats:p><\/jats:sec>","DOI":"10.1111\/ceo.13579","type":"journal-article","created":{"date-parts":[[2019,7,2]],"date-time":"2019-07-02T14:06:36Z","timestamp":1562076396000},"page":"1074-1081","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":29,"title":["Effects of VEGF inhibitors on human retinal pigment epithelium under high glucose and hypoxia"],"prefix":"10.1111","volume":"47","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1057-5142","authenticated-orcid":false,"given":"Bobak","family":"Bahrami","sequence":"first","affiliation":[{"name":"Save Sight Institute University of Sydney  Sydney New South Wales Australia"},{"name":"Sydney Institute of Vision Science  Sydney New South Wales 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