{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,23]],"date-time":"2026-04-23T06:46:51Z","timestamp":1776926811325,"version":"3.51.2"},"reference-count":36,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2019,10,22]],"date-time":"2019-10-22T00:00:00Z","timestamp":1571702400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clinical Exper Ophthalmology"],"published-print":{"date-parts":[[2020,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p><jats:italic>Mycobacterium tuberculosis<\/jats:italic> (MTB) in latent infection has been demonstrated in pulmonary\/extra\u2010pulmonary locations (lung, spleen, liver, kidney, adipose tissue) in autopsy studies, but its presence in ocular tissues in the latent state is not known.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We conducted molecular and histopathological study of 100 cadaver eyes (50 humans) who died from causes other than tuberculosis (TB) (and were potential candidates for corneal transplantation) to detect MTB in ocular tissues in an endemic setting. After removal of the corneal button, an 8 to 10\u2009mm block of tissue (choroid, retina and part of the vitreous) was excised from the remaining globe for DNA isolation. Gel\u2010based IS6110 and devR3 polymerase chain reaction (PCR) assays were done, followed by real\u2010time PCR using beta actin gene as an internal control. Sixteen randomly selected DNA samples were double checked using a commercial kit for MTB and non\u2010tuberculous mycobacteria (NTM) DNA. The remaining larger part of the globe was subjected to histopathology.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The mean age was 65.14\u2009\u00b1\u200918\u2009years. All 100 samples were negative for both IS6110 and devR, and all 16 samples were negative with NTM MTB commercial kit. All samples were negative with Ziehl\u2010Neelsen stain for acid fast bacilli and none showed any inflammation or granulomatous pathology.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>MTB could not be detected in human ocular tissues in latent state in India, a TB\u2010endemic country. This may suggest the inability of MTB to seed ocular tissues in the latent state, unlike other organs which serve as reservoirs for the bacilli in the absence of manifest disease.<\/jats:p><\/jats:sec>","DOI":"10.1111\/ceo.13654","type":"journal-article","created":{"date-parts":[[2019,10,6]],"date-time":"2019-10-06T07:55:44Z","timestamp":1570348544000},"page":"69-77","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["<i>Mycobacterium tuberculosis<\/i> does not show evidence of molecular DNA in human cadaveric ocular tissues in an endemic setting"],"prefix":"10.1111","volume":"48","author":[{"given":"Harish","family":"Chauhan","sequence":"first","affiliation":[{"name":"Advanced Eye Centre, Post Graduate Institute of Medical Education and Research  Chandigarh 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