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In retinal organ cultures, H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub> induces strong neurodegeneration of the retina. It is known that oxidative stress plays a role in the development of several retinal diseases including glaucoma and ischemia. Thus, we investigated whether processes underlying oxidative stress can be prevented by hypothermia using an ex vivo organ culture model of porcine retinas.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Porcine retinal explants were cultivated for 5 and 8\u2009days. Oxidative stress was induced via 300\u2009\u03bcM H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub> on day 1 for 3\u2009hours. Hypothermia treatment at 30\u00b0C was applied simultaneously with H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub>, for 3\u2009hours. Retinal ganglion cells (RGCs), apoptosis, bipolar and cholinergic amacrine cells, microglia and macroglia were evaluated immunohistologically. Apoptosis rate was additionally analysed via western blot.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Reduced apoptosis rates through hypothermia led to a preservation of RGCs (<jats:italic>P<\/jats:italic>\u2009&lt;\u2009.001). Amacrine cells were rescued after hypothermia treatment (<jats:italic>P<\/jats:italic> = .17), whereas bipolar cells were only protected partly. Additionally, at 8\u2009days, microglial response due to oxidative stress was completely counteracted via hypothermia (<jats:italic>P<\/jats:italic>\u2009&lt;\u2009.001).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub> induced strong degenerative processes in porcine retinas. The role of oxidative stress in the progression of retinal diseases makes this ex vivo organ culture model suitable to investigate new therapeutic approaches. In the present study, the damaging effect of H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub> to several retinal cell types was counteracted or strongly alleviated through hypothermia treatment. Especially RGCs, which are affected in glaucoma disease, were protected due to a reduced apoptosis rate through hypothermia.<\/jats:p><\/jats:sec>","DOI":"10.1111\/ceo.13731","type":"journal-article","created":{"date-parts":[[2020,2,20]],"date-time":"2020-02-20T07:32:23Z","timestamp":1582183943000},"page":"666-681","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Oxidative stress\u2010induced retinal damage is prevented by mild hypothermia in an ex vivo model of cultivated porcine retinas"],"prefix":"10.1111","volume":"48","author":[{"given":"Ana M.","family":"Mueller\u2010Buehl","sequence":"first","affiliation":[{"name":"Experimental Eye Research Institute University Eye Hospital, Ruhr\u2010University Bochum  Bochum Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hannah","family":"Doepper","sequence":"additional","affiliation":[{"name":"Experimental Eye Research Institute University Eye Hospital, Ruhr\u2010University Bochum  Bochum Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sven","family":"Grauthoff","sequence":"additional","affiliation":[{"name":"Experimental Eye Research Institute University Eye Hospital, Ruhr\u2010University Bochum  Bochum Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tobias","family":"Kiebler","sequence":"additional","affiliation":[{"name":"Centre for Ophthalmology T\u00fcbingen University Eye Hospital T\u00fcbingen  T\u00fcbingen Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Laura","family":"Peters","sequence":"additional","affiliation":[{"name":"Experimental Eye Research Institute University Eye Hospital, Ruhr\u2010University Bochum  Bochum Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jos\u00e9","family":"Hurst","sequence":"additional","affiliation":[{"name":"Centre for Ophthalmology T\u00fcbingen University Eye Hospital T\u00fcbingen  T\u00fcbingen Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sandra","family":"Kuehn","sequence":"additional","affiliation":[{"name":"Experimental Eye Research Institute University Eye Hospital, Ruhr\u2010University Bochum  Bochum Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Karl U.","family":"Bartz\u2010Schmidt","sequence":"additional","affiliation":[{"name":"Centre for Ophthalmology T\u00fcbingen University Eye Hospital T\u00fcbingen  T\u00fcbingen Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H. 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