{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,5,14]],"date-time":"2025-05-14T00:51:16Z","timestamp":1747183876777,"version":"3.40.5"},"reference-count":50,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2020,10,19]],"date-time":"2020-10-19T00:00:00Z","timestamp":1603065600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clinical Exper Ophthalmology"],"published-print":{"date-parts":[[2020,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Retinopathy of prematurity (ROP), a major cause of significant visual morbidity and blindness in preterm infants, is closely related to pathological angiogenesis. The aim of the study is to evaluate the effect of a new 12\u2010aa peptide (named peptide CW\u2010703) from human insulin\u2010like growth factor\u20102, against angiogenesis in ROP.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>In order to evaluate the inhibitory effect of CW\u2010703 on the proliferation, migration, tube formation and apoptosis of human umbilical vein endothelial cells (ScienCell) in vitro, we used MTS assays, a modified Boyden chamber, Matrigel system and TUNEL assays. Effects in vivo were assayed using chorioallantoic membrane assays and oxygen\u2010induced retinopathy (OIR) models in mice. We also performed eletrophysiological and histologic examinations to evaluate the possible toxicity of the peptide. Real\u2010time PCR, ELISA and western blotting were used to elucidate the mechanism of CW\u2010703.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>CW\u2010703 inhibited angiogenesis in vitro by suppressing endothelial cell proliferation, migration and tube formation. CW\u2010703 also prevented angiogenesis in chicken chorioallantoic membrane assays and OIR assays in mice. No evident functional or morphologic abnormalities in neuroretina after CW\u2010703 injection were revealed in electrophysiological tests and histological examinations. Moreover, we elucidated that CW\u2010703 competed for binding to IGF\u20101R and inhibited angiogenesis by inhibiting IGF\u20101R\/PI3K\/AKT activation and downregulating vascular endothelial growth factor expression.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The novel peptide CW\u2010703 may act as an effective inhibitor of ocular pathologic angiogenesis, especially in treating ROP.<\/jats:p><\/jats:sec>","DOI":"10.1111\/ceo.13864","type":"journal-article","created":{"date-parts":[[2020,10,7]],"date-time":"2020-10-07T15:50:10Z","timestamp":1602085810000},"page":"1261-1275","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Novel peptide derived from <scp>IGF<\/scp>\u20102 displays anti\u2010angiogenic activity in vitro and inhibits retinal angiogenesis in a model of oxygen\u2010induced 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