{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,20]],"date-time":"2025-12-20T02:21:17Z","timestamp":1766197277653,"version":"3.40.5"},"reference-count":25,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2022,9,29]],"date-time":"2022-09-29T00:00:00Z","timestamp":1664409600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clinical Exper Ophthalmology"],"published-print":{"date-parts":[[2023,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>To explore the use of a thermoreversible copolymer gel coating to prevent donor tissue scrolling in Descemet's membrane endothelial keratoplasty <jats:bold>(<\/jats:bold>DMEK).<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>PLGA\u2010PEG\u2010PLGA triblock copolymer was synthesised via ring opening polymerisation. Two formulations were fabricated and gelation properties characterised using rheological analyses. Endothelial cytotoxicity of the copolymer was assessed using a Trypan Blue exclusion assay. Thickness of the copolymer gel coating on the endothelial surface was analysed using anterior segment optical coherence tomography (OCT) (RTVue\u2010100, Optovue Inc.). Gold nanoparticles were added to the copolymer to aid visualisation using OCT. Prevention of Descemet membrane donor scrolling was represented via a novel, in vitro, immersion of copolymer coated donor graft material.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Two different formulations of PLGA\u2010PEG\u2010PLGA copolymer were successfully fabricated and the desired peak gelling temperature of 24\u00b0C was achieved by polymer blending. Application of 20%, 30% and 40% (wt\/vol) polymer concentrations resulted in a statistically significant increase in polymer thickness on the endothelium (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.001). There was no detectable endothelial cytotoxicity. The polymer was easy to apply to the endothelium and prevented scrolling of the DMEK graft.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>This PLGA\u2010PEG\u2010PLGA thermoreversible copolymer gel could be exploited as a therapeutic aid for preventing DMEK graft scrolling.<\/jats:p><\/jats:sec>","DOI":"10.1111\/ceo.14167","type":"journal-article","created":{"date-parts":[[2022,9,10]],"date-time":"2022-09-10T09:04:05Z","timestamp":1662800645000},"page":"58-66","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["An in vitro assessment of the thermoreversible <scp>PLGA\u2010PEG\u2010PLGA<\/scp> copolymer: Implications for <scp>Descemet<\/scp>'s membrane endothelial keratoplasty"],"prefix":"10.1111","volume":"51","author":[{"given":"Naing L.","family":"Tint","sequence":"first","affiliation":[{"name":"Moorfields Eye Hospital NHS Foundation Trust Cornea and External Eye Disease Service  London UK"},{"name":"Princess Alexandra Eye Pavilion NHS Lothian  Edinburgh UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5618-082X","authenticated-orcid":false,"given":"Kelvin K. 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