{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,11]],"date-time":"2026-05-11T11:38:36Z","timestamp":1778499516415,"version":"3.51.4"},"reference-count":28,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2025,7,16]],"date-time":"2025-07-16T00:00:00Z","timestamp":1752624000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/100006312","name":"BrightFocus Foundation","doi-asserted-by":"publisher","award":["G2024011S"],"award-info":[{"award-number":["G2024011S"]}],"id":[{"id":"10.13039\/100006312","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clinical Exper Ophthalmology"],"published-print":{"date-parts":[[2025,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>The success of minimally invasive bleb surgery (MIBS) such as the PreserFlo MicroShunt (PFMS) is limited by post\u2010operative bleb fibrosis. Current clinical practice prescribes the use of off\u2010label cytotoxic antimetabolites such as Mitomycin C (MMC) as antifibrotic therapy. This study investigates the anti\u2010scarring properties of a novel compound, 3\u2032,4\u2032\u2010Dihydroxyflavonol (DiOHF) in a rabbit model of PFMS filtration surgery.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      Fifteen New Zealand white rabbits underwent unilateral MIBS with PFMS. Treatment was randomised to the following groups: (1) vehicle (\n                      <jats:italic>n<\/jats:italic>\n                      \u2009=\u20095), (2) DiOHF (\n                      <jats:italic>n<\/jats:italic>\n                      \u2009=\u20095), both applied three times daily for 2\u2009weeks or (3) a single dose of MMC administered intraoperatively (\n                      <jats:italic>n<\/jats:italic>\n                      \u2009=\u20095). Blebs were photographed post\u2010operatively on Days 0, 7 and 14 for clinical examination. Eyes were harvested on Day 14 and processed for immunohistochemical tissue staining to assess collagen deposition, expression of \u03b1SMA, oxidative stress, fibroblast activity, angiogenesis and inflammation in the conjunctiva\/Tenon's layer.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      At 2\u2010weeks post\u2010surgery, MMC blebs were larger and more ischaemic than other treatments (Bleb size: MMC vs. DiOHF,\n                      <jats:italic>p<\/jats:italic>\n                      \u2009=\u20090.0098; MMC vs. vehicle,\n                      <jats:italic>p<\/jats:italic>\n                      \u2009=\u20090.0001. Ischaemia:\n                      <jats:italic>p<\/jats:italic>\n                      \u2009=\u20090.0004 for both). Compared to vehicle control, DiOHF blebs expressed decreased vimentin (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009=\u20090.0099), \u03b1SMA (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009=\u20090.0231) and 3\u2010nitrotyrosine (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009=\u20090.0410) after 2\u2009weeks. DiOHF blebs expressed less CD45 than other treatments (DiOHF vs. Vehicle,\n                      <jats:italic>p<\/jats:italic>\n                      \u2009=\u20090.0013; DiOHF vs. MMC,\n                      <jats:italic>p<\/jats:italic>\n                      \u2009=\u20090.0232) and less collagen accumulation (DiOHF vs. vehicle,\n                      <jats:italic>p<\/jats:italic>\n                      \u2009=\u20090.0512). There was no difference in CD31 expression between treatment groups.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>DiOHF inhibited scarring following implantation of PFMS in rabbit eyes by reducing local oxidative stress, inflammation and fibroblastic activity. DiOHF may be a safer and more effective wound modulating agent than the conventional use of MMC.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/ceo.14578","type":"journal-article","created":{"date-parts":[[2025,7,17]],"date-time":"2025-07-17T04:46:40Z","timestamp":1752727600000},"page":"986-995","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Inhibitory Effects of 3\u2032,4\u2032\u2010Dihydroxyflavonol in a Rabbit Model of Minimally Invasive Bleb Surgery With\n                    <scp>PreserFlo MicroShunt<\/scp>"],"prefix":"10.1111","volume":"53","author":[{"given":"Zoe","family":"Pasvanis","sequence":"first","affiliation":[{"name":"Centre for Eye Research Australia Royal Victorian Eye and Ear Hospital  East Melbourne Victoria Australia"},{"name":"Ophthalmology, Department of Surgery University of Melbourne  Parkville Victoria Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Roy C. K.","family":"Kong","sequence":"additional","affiliation":[{"name":"Centre for Eye Research Australia Royal Victorian Eye and Ear Hospital  East Melbourne Victoria Australia"},{"name":"Ophthalmology, Department of Surgery University of Melbourne  Parkville Victoria Australia"},{"name":"Department of Medicine, St. Vincent's Hospital University of Melbourne  Parkville Victoria Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elsa C.","family":"Chan","sequence":"additional","affiliation":[{"name":"Centre for Eye Research Australia Royal Victorian Eye and Ear Hospital  East Melbourne Victoria Australia"},{"name":"Ophthalmology, Department of Surgery University of Melbourne  Parkville Victoria Australia"},{"name":"Department of Medicine, St. Vincent's Hospital University of Melbourne  Parkville Victoria Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2782-9700","authenticated-orcid":false,"given":"Jennifer C.","family":"Fan Gaskin","sequence":"additional","affiliation":[{"name":"Centre for Eye Research Australia Royal Victorian Eye and Ear Hospital  East Melbourne Victoria Australia"},{"name":"Ophthalmology, Department of Surgery University of Melbourne  Parkville Victoria Australia"},{"name":"Glaucoma Research and Investigation Unit Royal Victorian Eye and Ear Hospital  East Melbourne Victoria Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,7,16]]},"reference":[{"key":"e_1_2_8_2_1","unstructured":"GlaucomaAustralia \u201cWhat Is Glaucoma? [Internet] \u201d(2024) Glaucoma Australia https:\/\/glaucoma.org.au\/what\u2010is\u2010glaucoma."},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcjo.2016.07.013"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.5041\/RMMJ.10345"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI31487"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1076\/ceyr.27.2.35.15456"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1001\/archopht.1983.01040010795021"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajo.2011.10.026"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijerph20042904"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1001\/archopht.124.12.1742"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1093\/rb\/rbw005"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1007\/s40123-022-00529-4"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ogla.2023.04.002"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ophtha.2021.05.023"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00417\u2010024\u201006649\u2010w"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1097\/00005344-200002000-00023"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1167\/tvst.11.8.18"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms251910767"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1177\/0022034509359125"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.redox.2015.09.009"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0955-2863(02)00208-5"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.3390\/cells10071587"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1186\/s11658-017-0034-4"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1524-4725.2008.34254.x"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.0000187457.24338.3D"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1161\/ATVBAHA.107.151456"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1042\/CS20140714"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.15-16595"},{"issue":"8","key":"e_1_2_8_29_1","first-page":"5296","article-title":"Differences in Tenon's Capsule Fascia With Aging and Prior Ocular Surgery","volume":"64","author":"Hammond A.","year":"2023","journal-title":"Investigative Ophthalmology & Visual Science"}],"container-title":["Clinical &amp; Experimental Ophthalmology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/ceo.14578","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,11,9]],"date-time":"2025-11-09T04:26:10Z","timestamp":1762662370000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/ceo.14578"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,7,16]]},"references-count":28,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2025,11]]}},"alternative-id":["10.1111\/ceo.14578"],"URL":"https:\/\/doi.org\/10.1111\/ceo.14578","archive":["Portico"],"relation":{},"ISSN":["1442-6404","1442-9071"],"issn-type":[{"value":"1442-6404","type":"print"},{"value":"1442-9071","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,7,16]]},"assertion":[{"value":"2024-11-15","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-06-20","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-07-16","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}