{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T05:21:42Z","timestamp":1791177702700,"version":"4.1.0"},"reference-count":50,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2025,7,14]],"date-time":"2025-07-14T00:00:00Z","timestamp":1752451200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81970783"],"award-info":[{"award-number":["81970783"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clinical Exper Ophthalmology"],"published-print":{"date-parts":[[2025,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Posterior capsule opacification (PCO), the most common complication following cataract surgery, results from the proliferation, migration and epithelial\u2013mesenchymal transition (EMT) of residual lens epithelial cells (LECs), typically driven by postoperative inflammatory and growth factor changes. However, delayed\u2010onset PCO, occurring years after surgery without obvious inflammation, suggests the involvement of additional mechanisms, with senescent cells identified as contributors to EMT. This study investigates the role of LEC senescence in PCO development.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Transcriptomic data from the Gene Expression Omnibus (GEO) were analysed to identify senescence\u2010associated pathways in PCO. Rabbit PCO models and oxidative stress\u2010induced senescent SRA01\/04 LECs were treated with dasatinib and quercetin (DQ) to target senescent cells and inhibit the senescence\u2010associated secretory phenotype (SASP). Senescence, SASP and EMT biomarkers were assessed by qPCR, immunofluorescence and Western blotting. Cell migration, proliferation and apoptosis were analysed through wound healing, Transwell and flow cytometry assays.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>RNA sequencing from GEO revealed a significant correlation between senescence and PCO. In\u00a0vitro, oxidative stress\u2010induced senescent LECs exhibited increased EMT biomarkers, including vimentin and \u03b1\u2010SMA. In a rabbit PCO model, senescence\u2010 and SASP\u2010related genes (p53, MMP3 and IL\u20106) and proteins were upregulated. DQ treatment reduced senescence and EMT by inhibiting multiple EMT\u2010related signalling pathways, especially the PI3K\u2010Akt pathway (p\u2010PI3K, p\u2010Akt), leading to a significant reduction in PCO volume.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>LEC senescence plays a key role in PCO development. DQ effectively targets multiple EMT signalling pathways, particularly PI3K\u2010Akt, and shows promise as a strategy for the prevention and management of PCO.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/ceo.14582","type":"journal-article","created":{"date-parts":[[2025,7,15]],"date-time":"2025-07-15T05:15:04Z","timestamp":1752556504000},"page":"1008-1024","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Novel Insight of Posterior Capsule Opacification: The Role of Lens Epithelial Cell Senescence"],"prefix":"10.1111","volume":"53","author":[{"given":"Yu","family":"Ma","sequence":"first","affiliation":[{"name":"State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Guangdong Provincial Key Laboratory of Ophthalmology and Visual Science Sun\u00a0Yat\u2010Sen University  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zewen","family":"Ren","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Guangdong Provincial Key Laboratory of Ophthalmology and Visual Science Sun\u00a0Yat\u2010Sen University  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yang","family":"Chen","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Guangdong Provincial Key Laboratory of Ophthalmology and Visual Science Sun\u00a0Yat\u2010Sen University  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lu","family":"Qin","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Guangdong Provincial Key Laboratory of Ophthalmology and Visual Science Sun\u00a0Yat\u2010Sen University  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rong","family":"Ju","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Guangdong Provincial Key Laboratory of Ophthalmology and Visual Science Sun\u00a0Yat\u2010Sen University  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yingyan","family":"Qin","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Guangdong Provincial Key Laboratory of Ophthalmology and Visual Science Sun\u00a0Yat\u2010Sen University  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6355-265X","authenticated-orcid":false,"given":"Mingxing","family":"Wu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Guangdong Provincial Key Laboratory of Ophthalmology and Visual Science Sun\u00a0Yat\u2010Sen University  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,7,14]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcrs.2011.05.035"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41433\u2010019\u20100630\u20109"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1080\/03007995.2019.1647012"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.preteyeres.2020.100905"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.18\u201025067"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmed.2024.1301588"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcrs.2018.09.022"},{"key":"e_1_2_7_9_1","first-page":"1130","article-title":"Effect of Intraocular Surgery and Ketamine on Aqueous and Serum Cytokines","volume":"13","author":"Tu K. 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