{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,29]],"date-time":"2025-12-29T19:38:01Z","timestamp":1767037081702,"version":"3.48.0"},"reference-count":41,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2025,9,19]],"date-time":"2025-09-19T00:00:00Z","timestamp":1758240000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100011912","name":"Taipei Veterans General Hospital","doi-asserted-by":"publisher","award":["R1900501"],"award-info":[{"award-number":["R1900501"]}],"id":[{"id":"10.13039\/501100011912","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clinical Exper Ophthalmology"],"published-print":{"date-parts":[[2025,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>\n                      To investigate\n                      <jats:italic>BRAF<\/jats:italic>\n                      mutation status and its associated clinicopathological features in conjunctival melanocytic lesions.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>We analysed surgical specimens from 79 conjunctival melanocytic lesions, including inflamed juvenile conjunctival nevi, primary acquired melanosis (PAM) and conjunctival common nevi collected from 2013 to 2022. PAM lesions were further categorised into those without atypia and with mild, moderate or severe atypia. Conjunctival common nevi were subcategorized into junctional, subepithelial and compound nevi. BRAF V600E immunohistochemistry was performed on all specimens, with PCR\u2010mass spectrometry used for ambiguous cases. The main outcome measures were the BRAF V600E mutation rate and related clinicopathological features.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Inflamed juvenile conjunctival nevi showed a BRAF V600E mutation rate of 36.8% (7\/19), while the mutation was rare in PAM (4.3%, 1\/23). The mutation rate in conjunctival common nevi (67.6%, 25\/37) was similar to that in cutaneous common nevi, and most BRAF V600E\u2010mutated cases were found in the subepithelial nevus group (93.8%, 15\/16).\n                      <jats:italic>BRAF<\/jats:italic>\n                      mutations were significantly associated with intralesional inflammatory stroma and subepithelial growth pattern, but not with age, lesion location, intralesional cyst and solar elastosis.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>\n                      <jats:italic>BRAF<\/jats:italic>\n                      mutations are key driver mutations in inflamed juvenile conjunctival nevi and conjunctival common nevi. Histologically, an \u2018inflamed juvenile conjunctival nevus\u2019\u2010like inflammatory stroma correlated with\n                      <jats:italic>BRAF<\/jats:italic>\n                      mutations. In our study, solar elastosis was infrequently observed in conjunctival nevi, suggesting that the mutational signature of conjunctival melanocytic lesions resembles that of cutaneous non\u2010 or low\u2010level cumulative solar damage type. For cases with equivocal BRAF staining, high\u2010sensitivity sequencing is recommended to confirm mutation status.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/ceo.14599","type":"journal-article","created":{"date-parts":[[2025,9,19]],"date-time":"2025-09-19T08:27:11Z","timestamp":1758270431000},"page":"1191-1201","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["<scp>\n                      <i>BRAF<\/i>\n                    <\/scp>\n                    Mutation Status in Inflamed Juvenile Conjunctival Nevus, Primary Acquired Melanosis and Conjunctival Common Nevus"],"prefix":"10.1111","volume":"53","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2250-0797","authenticated-orcid":false,"given":"Lei\u2010Chi","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Pathology and Laboratory Medicine Taipei Veterans General Hospital  Taipei Taiwan"},{"name":"School of Medicine National Yang Ming Chiao Tung University  Taipei Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wei\u2010Kuang","family":"Yu","sequence":"additional","affiliation":[{"name":"School of Medicine National Yang Ming Chiao Tung University  Taipei Taiwan"},{"name":"Department of Ophthalmology Taipei Veterans General Hospital  Taipei Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2354-2999","authenticated-orcid":false,"given":"Chih\u2010Chien","family":"Hsu","sequence":"additional","affiliation":[{"name":"School of Medicine National Yang Ming Chiao Tung University  Taipei Taiwan"},{"name":"Department of Ophthalmology Taipei Veterans General Hospital  Taipei Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yu\u2010Yun","family":"Huang","sequence":"additional","affiliation":[{"name":"School of Medicine National Yang Ming Chiao Tung University  Taipei Taiwan"},{"name":"Department of Ophthalmology Taipei Veterans General Hospital  Taipei Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chieh\u2010Chih","family":"Tsai","sequence":"additional","affiliation":[{"name":"School of Medicine National Yang Ming Chiao Tung University  Taipei Taiwan"},{"name":"Department of Ophthalmology Taipei Veterans General Hospital  Taipei Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tai\u2010Chi","family":"Lin","sequence":"additional","affiliation":[{"name":"School of Medicine National Yang Ming Chiao Tung University  Taipei Taiwan"},{"name":"Department of Ophthalmology Taipei Veterans General Hospital  Taipei Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,9,19]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa1002011"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature00766"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrm1498"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(04)00215-6"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/labinvest.2016.132"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/nri3789"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/ng1054"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-pathol-012513-104658"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.humpath.2014.04.001"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.17-22517"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/aos.13007"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.04-1449"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1442-9071.2008.01888.x"},{"volume-title":"Eye tumours [Internet; 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