{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,23]],"date-time":"2025-12-23T10:32:15Z","timestamp":1766485935815,"version":"3.40.5"},"reference-count":33,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2023,6,3]],"date-time":"2023-06-03T00:00:00Z","timestamp":1685750400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Congenital Anomalies"],"published-print":{"date-parts":[[2023,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Cleft lip and\/or palate anomalies (CL\u2009\u00b1\u2009P) are the most frequent birth defects affecting the orofacial region in humans. Although their etiology remains unclear, the involvement of environmental and genetic risk factors is known. This observational\u00a0study aimed to investigate how the use of\u00a0 crude drugs with estrogen activity influenced an animal model's ability to prevent CL\u2009\u00b1\u2009P. A\/J mice were randomly divided into six experimental groups. Five of these groups consumed a drink containing crude drug licorice root extract, with the following\u00a0weights attributed to each group: 3 g in group I, 6 g in group II, 7.5 g in group III, 9 g in group IV, and 12\u2009g in group V,\u00a0whereas a control group consumed tap water. The effect of licorice extract was examined for fetal mortality and fetal orofacial cleft development compared to the control group. The rates for fetal mortality\u00a0were 11.28%, 7.41%, 9.18%, 4.94%, and 7.90% in groups I, II, III, IV, and V, respectively, compared to 13.51% in the control group. There were no significant differences in the mean weight of alive fetuses in all five groups compared to the control group (0.63\u2009\u00b1\u20090.12). Group IV showed the lowest orafacial cleft occurrence of 3.20% (8 fetuses) with statistical significance <jats:italic>(p<\/jats:italic>\u2009=\u20090.0048) out of 268 live fetuses, whereas the control group had the occurrence of 8.75% (42 fetuses) among 480 live fetuses. Our study showed that the dried licorice root extract may reduce orofacial birth defects in experimental animal studies.<\/jats:p>","DOI":"10.1111\/cga.12527","type":"journal-article","created":{"date-parts":[[2023,6,3]],"date-time":"2023-06-03T07:44:27Z","timestamp":1685778267000},"page":"141-146","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Prevention of cleft lip and\/or palate in A\/J mice by licorice solution"],"prefix":"10.1111","volume":"63","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3969-3567","authenticated-orcid":false,"given":"Ichinnorov","family":"Chimedtseren","sequence":"first","affiliation":[{"name":"Division of Research and Treatment for Oral and Maxillofacial Congenital Anomalies Aichi Gakuin University, School of Dentistry  Nagoya Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Teruyuki","family":"Niimi","sequence":"additional","affiliation":[{"name":"Division of Research and Treatment for Oral and Maxillofacial Congenital Anomalies Aichi Gakuin University, School of Dentistry  Nagoya Japan"},{"name":"Cleft Lip and Palate Center Aichi Gakuin University Dental Hospital  Nagoya Japan"},{"name":"Division of Speech, Hearing, and Language Aichi Gakuin University Dental Hospital  Nagoya Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Makoto","family":"Inoue","sequence":"additional","affiliation":[{"name":"Laboratory of Medicinal Resources School of Pharmacy, Aichi Gakuin University  Nagoya Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hiroo","family":"Furukawa","sequence":"additional","affiliation":[{"name":"Cleft Lip and Palate Center Aichi Gakuin University Dental Hospital  Nagoya Japan"},{"name":"Division of Speech, Hearing, and Language Aichi Gakuin University Dental Hospital  Nagoya Japan"},{"name":"Department of Health Science, Faculty of Psychological and Physical Science Aichi Gakuin University  Nagoya Aichi Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0011-8223","authenticated-orcid":false,"given":"Hideto","family":"Imura","sequence":"additional","affiliation":[{"name":"Division of Research and Treatment for Oral and Maxillofacial Congenital Anomalies Aichi Gakuin University, School of Dentistry  Nagoya Japan"},{"name":"Cleft Lip and Palate Center Aichi Gakuin University Dental Hospital  Nagoya Japan"},{"name":"Division of Speech, Hearing, and Language Aichi Gakuin University Dental Hospital  Nagoya Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Katsuhiro","family":"Minami","sequence":"additional","affiliation":[{"name":"Division of Research and Treatment for Oral and Maxillofacial Congenital Anomalies Aichi Gakuin University, School of Dentistry  Nagoya Japan"},{"name":"Cleft Lip and Palate Center Aichi Gakuin University Dental Hospital  Nagoya Japan"},{"name":"Division of Speech, Hearing, and 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