{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,6]],"date-time":"2026-03-06T18:12:04Z","timestamp":1772820724191,"version":"3.50.1"},"reference-count":67,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2024,12,27]],"date-time":"2024-12-27T00:00:00Z","timestamp":1735257600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Congenital Anomalies"],"published-print":{"date-parts":[[2025,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    <jats:italic>Sonic hedgehog<\/jats:italic>\n                    (\n                    <jats:italic>Shh<\/jats:italic>\n                    ) is expressed in the oropharyngeal epithelium, including the frontonasal ectodermal zone (FEZ), which is defined as the boundary between\n                    <jats:italic>Shh<\/jats:italic>\n                    and\n                    <jats:italic>Fgf8<\/jats:italic>\n                    expression domains in the frontonasal epithelium. To investigate the role of SHH signaling from the oropharyngeal epithelium, we generated mice in which\n                    <jats:italic>Shh<\/jats:italic>\n                    expression is specifically deleted in the oropharyngeal epithelium (\n                    <jats:italic>Isl1\u2010Cre; Shh<\/jats:italic>\n                    <jats:sup>\n                      <jats:italic>f\/f<\/jats:italic>\n                    <\/jats:sup>\n                    ). In the mutant mouse,\n                    <jats:italic>Shh<\/jats:italic>\n                    expression was excised in the oropharyngeal epithelium as well as FEZ and ventral forebrain, consistent with the expression pattern of\n                    <jats:italic>Isl1<\/jats:italic>\n                    .\n                    <jats:italic>Isl1\u2010Cre; Shh<\/jats:italic>\n                    <jats:sup>\n                      <jats:italic>f\/f<\/jats:italic>\n                    <\/jats:sup>\n                    mice exhibited a complete loss of lower jaw components and a malformed upper jaw with defects in the cranial base and secondary palate. Massive cell death was observed in the mandibular process at embryonic day (E) 9.5 and E10.5, while mild cell death was observed in the lambdoidal region (the fusion area in the maxillary, lateral nasal, and medial nasal processes) at E10.5. An RNA\u2010seq analysis revealed that\n                    <jats:italic>Satb2<\/jats:italic>\n                    , a gene involved in cell survival during jaw formation, was downregulated in the lambdoidal region in\n                    <jats:italic>Isl1\u2010Cre; Shh<\/jats:italic>\n                    <jats:sup>\n                      <jats:italic>f\/f<\/jats:italic>\n                    <\/jats:sup>\n                    mice. These results suggest that\n                    <jats:italic>Shh<\/jats:italic>\n                    expression in the FEZ is required for cell survival and skeletogenesis in the lambdoidal region during the development of the upper jaw and that the developmental control governed by SHH signaling is different between upper and lower jaws.\n                  <\/jats:p>","DOI":"10.1111\/cga.70001","type":"journal-article","created":{"date-parts":[[2024,12,27]],"date-time":"2024-12-27T04:49:48Z","timestamp":1735274988000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["The role of sonic hedgehog signaling in the oropharyngeal epithelium during jaw development"],"prefix":"10.1111","volume":"65","author":[{"given":"Rika","family":"Takeuchi","sequence":"first","affiliation":[{"name":"Department of Molecular Craniofacial Embryology and Oral Histology, Graduate School of Medical and Dental Sciences Tokyo Medical and Dental 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Medical and Dental University (TMDU)  Tokyo Japan"},{"name":"Department of Anatomy, Faculty of Dentistry Chulalongkorn University  Bangkok Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Youichirou","family":"Ninomiya","sequence":"additional","affiliation":[{"name":"Research Center for Medical Bigdata, Research Organization of Information and Systems National Institute of Informatics  Tokyo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Toshiko","family":"Furutera","sequence":"additional","affiliation":[{"name":"Department of Molecular Craniofacial Embryology and Oral Histology, Graduate School of Medical and Dental Sciences Tokyo Medical and Dental University (TMDU)  Tokyo Japan"},{"name":"Department of Anatomy and Life Structure Juntendo University Graduate School of Medicine  Tokyo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kazushi","family":"Aoto","sequence":"additional","affiliation":[{"name":"Central Laboratory, Graduate School of Biomedical and Health Sciences Hiroshima University  Hiroshima Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daisuke","family":"Koyabu","sequence":"additional","affiliation":[{"name":"Department of Molecular Craniofacial Embryology and Oral Histology, Graduate School of Medical and Dental Sciences Tokyo Medical and Dental University (TMDU)  Tokyo Japan"},{"name":"Research and Development Center for Precision Medicine University of Tsukuba  Ibaraki Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Noritaka","family":"Adachi","sequence":"additional","affiliation":[{"name":"Department of Molecular Craniofacial Embryology and Oral Histology, Graduate School of Medical and Dental Sciences Tokyo Medical and Dental University (TMDU)  Tokyo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Katsuhiko","family":"Hayashi","sequence":"additional","affiliation":[{"name":"Department of Dentistry and 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