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We investigated 112 Greek HSP index cases collected over more than 25\u2009years from all regions of the country, using a combination of next generation sequencing and multiplex ligation\u2010dependent probe amplification. In total, we identified a causative variant in 68 patients, corresponding to a diagnostic yield of 60.7%. The diagnostic yield was 62.8% in autosomal dominant HSP, 77.8% in autosomal recessive HSP and 50.0% in sporadic cases. We identified 7 novel causative variants in\n                    <jats:italic>SPAST<\/jats:italic>\n                    ,\n                    <jats:italic>SPG7<\/jats:italic>\n                    , and\n                    <jats:italic>CYP7B1<\/jats:italic>\n                    . We found causative variants in a total of 18 different genes. The most commonly involved genes, affecting more than one family, were\n                    <jats:italic>SPAST<\/jats:italic>\n                    (25.0%),\n                    <jats:italic>SPG11<\/jats:italic>\n                    (12.5%),\n                    <jats:italic>CYP7B1<\/jats:italic>\n                    (3.6%),\n                    <jats:italic>SPG7<\/jats:italic>\n                    (3.6%),\n                    <jats:italic>KIF5A<\/jats:italic>\n                    (2.7%),\n                    <jats:italic>ABCD1<\/jats:italic>\n                    (1.8%) and\n                    <jats:italic>PSEN1<\/jats:italic>\n                    (1.8%). Variants in\n                    <jats:italic>ATL1<\/jats:italic>\n                    ,\n                    <jats:italic>REEP1<\/jats:italic>\n                    ,\n                    <jats:italic>BSCL2<\/jats:italic>\n                    ,\n                    <jats:italic>PLP1<\/jats:italic>\n                    ,\n                    <jats:italic>WASCHC5<\/jats:italic>\n                    ,\n                    <jats:italic>AP5Z1<\/jats:italic>\n                    ,\n                    <jats:italic>CYP27A1<\/jats:italic>\n                    ,\n                    <jats:italic>SOD1<\/jats:italic>\n                    ,\n                    <jats:italic>POLR3A, GFAP<\/jats:italic>\n                    and\n                    <jats:italic>ARG1<\/jats:italic>\n                    were identified in single families. This study presents a comprehensive overview of the phenotypic and genotypic spectrum of HSP in the Greek population, expanding previous data, and contributing to the characterisation of further pathogenic variants linked to HSP.\n                  <\/jats:p>","DOI":"10.1111\/cge.70113","type":"journal-article","created":{"date-parts":[[2025,11,24]],"date-time":"2025-11-24T09:51:37Z","timestamp":1763977897000},"page":"837-846","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["The Genetic Landscape of Hereditary Spastic Paraplegia in Greece"],"prefix":"10.1111","volume":"109","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8980-8377","authenticated-orcid":false,"given":"Georgios","family":"Koutsis","sequence":"first","affiliation":[{"name":"Neurogenetics Unit, 1st Department of Neurology, Eginition Hospital, National and Kapodistrian University of Athens  Athens 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