{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T16:32:45Z","timestamp":1777653165755,"version":"3.51.4"},"reference-count":20,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2016,12,12]],"date-time":"2016-12-12T00:00:00Z","timestamp":1481500800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clin Implant Dent Rel Res"],"published-print":{"date-parts":[[2017,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>The role of osteopontin (OPN) in the process of achieving osseointegration following implantation remains to be clarified.<\/jats:p><\/jats:sec><jats:sec><jats:title>Purpose<\/jats:title><jats:p>This study aimed to analyze the healing patterns of the bone\u2010implant interface after immediate placement of implants in the maxillae of 4\u2010week\u2010old <jats:italic>Opn<\/jats:italic>\u2010knockout (KO) and wild\u2010type (WT) mice.<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and Methods<\/jats:title><jats:p>After maxillary first molars were extracted, cavities were prepared with a drill and titanium implants blasted with ceramic abrasives containing hydroxyapatite\/\u03b2\u2010tricalcium phosphate were placed. Following fixation at 3, 5, 7, and 28 days after implantation, the samples were analyzed using immunohistochemistry, in situ hybridization, and an electron probe micro analyzer.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Two types of bone healing were observed in the process of achieving osseointegration: \u201cdirect osteogenesis,\u201d where bone formation occurs at the implant surface, and \u201cindirect osteogenesis,\u201d where it does at the pre\u2010existing damaged bone surface in the WT mice. Direct osteogenesis occurred after the recruitment of tartrate resistant acid phosphatase\u2010positive cells and the deposition of OPN on the implant surface. In contrast, the rate of osseointegration or direct osteogenesis was significantly low, and cell proliferation was disturbed in the <jats:italic>Opn<\/jats:italic>\u2010KO mice.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>These results suggest that <jats:italic>Opn<\/jats:italic>\u2010deficiency disturbs direct osteogenesis to lead the delayed osseointegration after immediate placement of endosseous implants.<\/jats:p><\/jats:sec>","DOI":"10.1111\/cid.12467","type":"journal-article","created":{"date-parts":[[2016,12,12]],"date-time":"2016-12-12T05:06:27Z","timestamp":1481519187000},"page":"496-504","source":"Crossref","is-referenced-by-count":9,"title":["<i>Osteopontin<\/i>\u2010deficiency disturbs direct osteogenesis in the process of achieving osseointegration following immediate placement of endosseous implants"],"prefix":"10.1111","volume":"19","author":[{"given":"Sanako","family":"Makishi","sequence":"first","affiliation":[{"name":"General Dentistry and Clinical Education Unit Niigata University Medical and Dental Hospital Niigata Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kotaro","family":"Saito","sequence":"additional","affiliation":[{"name":"Division of Anatomy and Cell Biology of the Hard Tissue, Department of Tissue Regeneration and Reconstruction Niigata University Graduate School of Medical and Dental Sciences Niigata Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hayato","family":"Ohshima","sequence":"additional","affiliation":[{"name":"Division of Anatomy and Cell Biology of the Hard Tissue, Department of Tissue Regeneration and Reconstruction Niigata University Graduate School of Medical and Dental Sciences Niigata Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2016,12,12]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0300-9785(81)80077-4"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9610(98)00183-4"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0501.2011.02327.x"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0501.2004.01082.x"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/cid.12280"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.0022-0337.2003.67.8.tb03681.x"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1902\/jop.2000.71.2.287"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.0905-7161.2003.00960.x"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biocel.2003.12.003"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1177\/10454411000110030101"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0029(19960201)33:2<141::AID-JEMT5>3.0.CO;2-W"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI2131"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00441-005-0138-4"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1177\/154405910608500506"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1267\/ahc.26.303"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.job.2014.06.005"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749-6632.1995.tb44632.x"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.4805-06.2007"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1242\/dmm.009993"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1359\/jbmr.1998.13.7.1101"}],"container-title":["Clinical Implant Dentistry and Related Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fcid.12467","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fcid.12467","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/cid.12467","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,3]],"date-time":"2023-10-03T18:32:45Z","timestamp":1696357965000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/cid.12467"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,12,12]]},"references-count":20,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2017,6]]}},"alternative-id":["10.1111\/cid.12467"],"URL":"https:\/\/doi.org\/10.1111\/cid.12467","archive":["Portico"],"relation":{},"ISSN":["1523-0899","1708-8208"],"issn-type":[{"value":"1523-0899","type":"print"},{"value":"1708-8208","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,12,12]]}}}