{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,1]],"date-time":"2026-04-01T17:50:54Z","timestamp":1775065854517,"version":"3.50.1"},"reference-count":28,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2020,10,15]],"date-time":"2020-10-15T00:00:00Z","timestamp":1602720000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clin Implant Dent Rel Res"],"published-print":{"date-parts":[[2020,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Purpose<\/jats:title><jats:p>To determine if healing abutments (HA) can be \u201cdecontaminated\u201d using four strategies available in clinical settings and compare the detoxification efficacy by quantifying residual biomaterial and capacity to elicit an inflammatory response in\u2010vitro.<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and methods<\/jats:title><jats:p>Forty HA collected from subjects following intraoral use were randomly distributed into four test groups (A\u2010D): A: autoclave only, B: ultrasonic bath plus autoclave, C: prophy\u2010jet plus autoclave, and D: Scrub sponge plus autoclave. New, sterile HA: group E (Control). Residual protein concentration was determined by Micro BCA assay and stained with Phloxine B for macroscopic examination. HA were placed in human CD14+ monocyte derived\u2010macrophage (mo\u2010M\u03c6) cultures and supernatant collected at 4, 24, 48, and 5\u2009days to analyze cytokine profiles using multiplex bead assay.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Test groups showed visible differences in \u201cdecontamination\u201d levels compared to control. Groups C and D showed most effective debris removal and lowest residual protein concentration. Multiplex assay showed marked induction of pro\u2010inflammatory cytokines by groups A and B and to a significantly lower level by groups C and D.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>HA were not entirely \u201cdecontaminated\u201d using common methods available relative to new, sterile HA and were capable of stimulating an immune response.<\/jats:p><\/jats:sec>","DOI":"10.1111\/cid.12956","type":"journal-article","created":{"date-parts":[[2020,10,16]],"date-time":"2020-10-16T05:56:27Z","timestamp":1602827787000},"page":"730-738","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Used dental implant healing abutments elicit immune responses: A comparative analysis of detoxification strategies"],"prefix":"10.1111","volume":"22","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8665-2699","authenticated-orcid":false,"given":"Aniruddh","family":"Narvekar","sequence":"first","affiliation":[{"name":"Department of Periodontics College of Dentistry, University of Illinois Chicago Illinois USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Araceli","family":"Valverde Estepa","sequence":"additional","affiliation":[{"name":"Department of Periodontics College of Dentistry, University of Illinois Chicago Illinois USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Afsar","family":"Naqvi","sequence":"additional","affiliation":[{"name":"Department of Periodontics College of Dentistry, University of Illinois Chicago Illinois USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Salvador","family":"Nares","sequence":"additional","affiliation":[{"name":"Department of Periodontics College of Dentistry, University of Illinois Chicago Illinois USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2020,10,15]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/cid.12385"},{"issue":"2","key":"e_1_2_10_3_1","doi-asserted-by":"crossref","first-page":"161","DOI":"10.11607\/ijp.5552","article-title":"Reuse of implant healing abutments: comparative evaluation of the efficacy of two cleaning procedures","volume":"31","author":"Stacchi C","year":"2018","journal-title":"Int J Prosthodont"},{"issue":"5","key":"e_1_2_10_4_1","doi-asserted-by":"crossref","first-page":"419","DOI":"10.1080\/19424396.2012.12220911","article-title":"Sterilization analysis of contaminated healing abutments and impression copings","volume":"40","author":"Browne V","year":"2012","journal-title":"J Calif Dent Assoc"},{"key":"e_1_2_10_5_1","unstructured":"Cleaning and sterilization instructions for Astra Tech Implant System\u00ae EV Dentsply Sirona Implants.2017.https:\/\/assets.dentsplysirona.com\/dentsply\/pim\/manufacturer\/Implants\/Implant_systems\/Astra_Tech_Implant_System_EV\/Instruments\/Accessories\/32671332-USX-1711%20Cleaning%20and%20sterilization%20instructions%20for%20Astra%20Tech%20Implant%20System%20EV_LR.pdf"},{"issue":"3","key":"e_1_2_10_6_1","doi-asserted-by":"crossref","first-page":"234","DOI":"10.11138\/orl\/2017.10.3.234","article-title":"A new system of implant abutment connection: how to improve a two\u2010piece implant system sealing","volume":"10","author":"Grecchi F","year":"2017","journal-title":"Oral Implantol (Rome)"},{"issue":"5","key":"e_1_2_10_7_1","first-page":"e583","article-title":"Is the re\u2010use of sterilized implant abutments safe enough? 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