{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T13:26:33Z","timestamp":1785417993329,"version":"3.56.0"},"reference-count":38,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2022,6,24]],"date-time":"2022-06-24T00:00:00Z","timestamp":1656028800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clin Implant Dent Rel Res"],"published-print":{"date-parts":[[2022,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Aim<\/jats:title><jats:p>To compare the clinical and radiographic conditions and the expression of pro\u2010inflammatory cytokines in peri\u2010implant crevicular fluid (PICF) at two\u2010piece\/bone level (TP\/BL) versus one\u2010piece\/tissue level (OP\/TL) single implants with a laser\u2010microgrooved collar after at least 5\u2009years of loading.<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and Methods<\/jats:title><jats:p>In total, 20 single TP\/BL implants and 20 contralateral OP\/TL implants, both with a laser\u2010microgrooved collar surface, in 20 systemically and periodontally healthy subjects (12 males and 8 females, between the age of 36 and 64 [mean age of 49.7\u00a0\u00b1\u200912.3\u2009years]), were examined. Levels of IL\u20101\u03b2, IL\u20101RA, IL\u20106, IL\u20108, IL\u201017, b\u2010FGF, G\u2010CSF, GM\u2010CSF, IFN, MIP\u20101\u03b2, TNF\u2010\u03b1, and VEGF were assessed in PICF using the Bio\u2010Plex 200 Suspension Array System. Plaque index (PI), probing depth (PD), bleeding on probing (BOP), and gingival recession (REC) were recorded. Radiographic crestal bone levels (CBL) were assessed at the mesial and distal aspects of the implant sites.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The mean PI, PD, BOP, and REC values had no significant differences in either group. A higher mean value of CBL with statistical difference was detected for TP\/BL compared with OP\/TL implants. The levels of IL\u20101\u03b2, IL\u20106, IL\u20108, GM\u2010CSF, and MIP\u20101\u03b2 and TNF\u2010\u03b1 were higher at TP\/BL implants than at OP\/TL implants. However, only IL\u20101\u03b2, IL\u20106, and TNF\u2010\u03b1 values presented significant differences between the groups.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Although after 5\u2009years of loading single TP\/BL and OP\/TL implants with a laser\u2010microgrooved collar surface presented similar good clinical conditions, a higher proinflammatory state and higher crestal bone loss were detected for TP\/BL implants.<\/jats:p><\/jats:sec>","DOI":"10.1111\/cid.13118","type":"journal-article","created":{"date-parts":[[2022,6,24]],"date-time":"2022-06-24T15:33:23Z","timestamp":1656084803000},"page":"676-682","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":31,"title":["Clinical, radiographic, and biochemical evaluation of two\u2010piece versus one\u2010piece single implants with a laser\u2010microgrooved collar surface after 5\u2009years of functional loading"],"prefix":"10.1111","volume":"24","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1449-8340","authenticated-orcid":false,"given":"Renzo","family":"Guarnieri","sequence":"first","affiliation":[{"name":"Department of Oral and Maxillofacial Sciences Sapienza University of Rome  Rome Italy"},{"name":"Private Periodontal\u2010Implant Practice  Treviso Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rodolfo","family":"Reda","sequence":"additional","affiliation":[{"name":"Department of Oral and Maxillofacial Sciences Sapienza University of Rome  Rome Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dario","family":"Di Nardo","sequence":"additional","affiliation":[{"name":"Department of Oral and Maxillofacial Sciences Sapienza University of Rome  Rome Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gabriele","family":"Miccoli","sequence":"additional","affiliation":[{"name":"Department of Oral and Maxillofacial 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