{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T20:01:53Z","timestamp":1790625713288,"version":"4.1.0"},"reference-count":28,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2023,1,11]],"date-time":"2023-01-11T00:00:00Z","timestamp":1673395200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100002873","name":"Chulalongkorn University","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002873","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clin Implant Dent Rel Res"],"published-print":{"date-parts":[[2023,4]]},"abstract":"Abstract<\/jats:title>\n \n Purpose<\/jats:title>\n \n The aim of this study was to investigate the effects of deproteinized bovine bone (DBB, Bio\u2010Oss\u00ae) and freeze\u2010dried bone allograft (FDBA, SureOss\u00ae) on bone healing during maxillary sinus floor augmentation (MSFA) using histology, immunohistochemistry, and gene expressions of the marker genes including\n Runx2<\/jats:italic>\n ,\n Opn<\/jats:italic>\n ,\n Ocn<\/jats:italic>\n ,\n Col1a1<\/jats:italic>\n ,\n Rankl<\/jats:italic>\n , and\n Tnf\u2010\u03b1<\/jats:italic>\n .\n <\/jats:p>\n <\/jats:sec>\n \n Materials and Methods<\/jats:title>\n \n Fourteen participants who required two\u2010stage maxillary sinus augmentation were randomly assigned to DBB and FDBA bone grafting groups. Six months after the sinus augmentation procedure, bone samples were collected before implant placement with a trephine bur. Gene expression of\n Runx2<\/jats:italic>\n ,\n Opn<\/jats:italic>\n ,\n Ocn<\/jats:italic>\n ,\n Col1a1<\/jats:italic>\n ,\n Rankl<\/jats:italic>\n , and\n Tnf\u2010\u03b1<\/jats:italic>\n of the bone samples was assessed by real\u2010time polymerase chain reaction as a primary outcome. Histological analysis of H&E\u2010stained sections, immunohistochemistry for OPN quantification, and CBCT\u2010based bone tissue examination were performed to investigate the bone healing effects of DBB and FDBA substitutes.\n <\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n \n The FDBA treated group showed higher gene expression when compared with the DBB treated group in\n Opn<\/jats:italic>\n (2.83\u2009\u00b1\u20091.23 vs. 1.40\u2009\u00b1\u20090.69;\n p<\/jats:italic>\n \u00a0=\u00a00.04),\n Runx2<\/jats:italic>\n (1.49\u2009\u00b1\u20090.44 vs. 0.67\u2009\u00b1\u20090.14;\n p<\/jats:italic>\n \u00a0=\u00a00.01), and\n Rankl<\/jats:italic>\n (2.34\u2009\u00b1\u20090.85 vs. 0.69\u2009\u00b1\u20090.39;\n p<\/jats:italic>\n \u00a0=\u00a00.03). In the DBB treated group a downregulated expression was found of\n Ocn<\/jats:italic>\n relative to maxillary edentulous bone (1.18\u2009\u00b1\u20090.40 vs. 2.51\u2009\u00b1\u20090.78;\n p<\/jats:italic>\n \u00a0=\u00a00.02).\n <\/jats:p>\n <\/jats:sec>\n \n Conclusion<\/jats:title>\n Two\u2010stage maxillary sinus augmentation with FDBA upregulated specific bone remodeling genes when compared to DBB. The outcome of gene expression matched with the ones for OPN immunoreactivity, being higher in the FDBA group. FDBA had an expression pattern similar to native bone and showed stronger expression of bone forming related\u2010genes suggesting it may be clinically preferable over DBB.<\/jats:p>\n This clinical trial was not registered prior to participant recruitment and randomization (clinical registration number TCTR20221217002).<\/jats:p>\n <\/jats:sec>","DOI":"10.1111\/cid.13179","type":"journal-article","created":{"date-parts":[[2023,1,11]],"date-time":"2023-01-11T04:32:59Z","timestamp":1673411579000},"page":"343-351","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Deproteinized bovine bone and freeze\u2010dried bone allograft in sinus floor augmentation: A randomized controlled trial"],"prefix":"10.1111","volume":"25","author":[{"given":"Isaya","family":"Kungvarnchaikul","sequence":"first","affiliation":[{"name":"Faculty of Dentistry Chulalongkorn University Bangkok Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1570-2289","authenticated-orcid":false,"given":"Keskanya","family":"Subbalekha","sequence":"additional","affiliation":[{"name":"Department of Oral and Maxillofacial Surgery, Faculty of Dentistry Chulalongkorn University Bangkok Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pimrumpai R.","family":"Sindhavajiva","sequence":"additional","affiliation":[{"name":"Department of Prosthodontics, Faculty of Dentistry Chulalongkorn University Bangkok Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jaijam","family":"Suwanwela","sequence":"additional","affiliation":[{"name":"Department of Prosthodontics, Faculty of Dentistry Chulalongkorn University Bangkok Thailand"},{"name":"Center of Excellence in Genomics and Precision Dentistry Faculty of Dentistry, Chulalongkorn University Bangkok 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