{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,17]],"date-time":"2026-06-17T07:09:13Z","timestamp":1781680153714,"version":"3.54.5"},"reference-count":51,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2024,10,22]],"date-time":"2024-10-22T00:00:00Z","timestamp":1729555200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clin Implant Dent Rel Res"],"published-print":{"date-parts":[[2025,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:sec><jats:title>Objective<\/jats:title><jats:p>The study evaluates three biologically active substances with known bone\u2010inductive potential on previously decontaminated titanium (Ti) discs.<\/jats:p><\/jats:sec><jats:sec><jats:title>Material and Methods<\/jats:title><jats:p>Rough and smooth Ti surfaces were contaminated with a multispecies biofilm and cleaned with a chitosan brush. Discs were treated either with injectable\u2010platelet\u2010rich fibrin (i\u2010PRF), advanced platelet\u2010rich fibrin (A\u2010PRF+), or enamel matrix derivatives (EMDs) before osteoblast seeding.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Biocompatibility, adhesion, migration, and gene expression of <jats:italic>runt\u2010related transcription factor 2<\/jats:italic> (<jats:italic>RUNX2<\/jats:italic>), <jats:italic>collagen Type I Alpha 2<\/jats:italic> (<jats:italic>COL1a2<\/jats:italic>), <jats:italic>alkaline phosphatase<\/jats:italic> (<jats:italic>ALP<\/jats:italic>), <jats:italic>osteocalcin<\/jats:italic> (<jats:italic>OC<\/jats:italic>), and <jats:italic>osteonectin<\/jats:italic> (<jats:italic>ON<\/jats:italic>) were performed. All the tested biologic agents similarly increased cell viability. Specifically, osteoblasts seeded over i\u2010PRF and EMD\u2010treated surfaces showed improvement in adhesion and migration and significantly increased <jats:italic>ALP, OC, ON, RUNX\u20102<\/jats:italic>, and <jats:italic>COL1a2<\/jats:italic> mRNA levels up to 2.8 fold (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.05) with no differences between Ti surfaces.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>i\u2010PRF and EMD possess beneficial bioactive properties that enhance tissue healing and promote regeneration on thoroughly sterilized surfaces. Biologically active materials may hold the potential to influence the process of implant re\u2010osseointegration, which warrants more research since sterilization of the affected surfaces under clinical conditions is still not reliably possible and remains one of the greatest challenges.<\/jats:p><\/jats:sec>","DOI":"10.1111\/cid.13406","type":"journal-article","created":{"date-parts":[[2024,10,22]],"date-time":"2024-10-22T23:28:49Z","timestamp":1729639729000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Effects of i\u2010<scp>PRF<\/scp>, A\u2010<scp>PRF<\/scp>+, and <scp>EMD<\/scp> on Osteogenic Potential of Osteoblasts on Titanium"],"prefix":"10.1111","volume":"27","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7732-2118","authenticated-orcid":false,"given":"Liza Lima","family":"Ramenzoni","sequence":"first","affiliation":[{"name":"Clinic of Conservative and Preventive Dentistry, Division of Periodontology and Peri\u2010Implant Diseases, Center of Dental Medicine University of Zurich  Zurich Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8503-5039","authenticated-orcid":false,"given":"Jothi","family":"Varghese","sequence":"additional","affiliation":[{"name":"Department of Periodontology, Manipal College of Dental Sciences, Manipal Manipal Academy of Higher Education  Manipal India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patrick Roger","family":"Schmidlin","sequence":"additional","affiliation":[{"name":"Clinic of Conservative and Preventive Dentistry, Division of Periodontology and Peri\u2010Implant Diseases, Center of Dental Medicine University of Zurich  Zurich Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shubhankar","family":"Mehrotra","sequence":"additional","affiliation":[{"name":"Department of Periodontology, Manipal College of Dental Sciences, Manipal Manipal Academy of Higher Education  Manipal India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,10,22]]},"reference":[{"key":"e_1_2_12_2_1","doi-asserted-by":"publisher","DOI":"10.1563\/AAID\u2010JOI\u2010D\u201012\u201000106"},{"key":"e_1_2_12_3_1","first-page":"345","article-title":"Localized Ridge Augmentation Using GBR and Platelet\u2010Rich Plasma: Case Reports","volume":"21","author":"Shanaman R.","year":"2001","journal-title":"International Journal of Periodontics & Restorative Dentistry"},{"key":"e_1_2_12_4_1","doi-asserted-by":"publisher","DOI":"10.1902\/jop.2000.71.10.1654"},{"key":"e_1_2_12_5_1","doi-asserted-by":"publisher","DOI":"10.4103\/1735\u20103327.166226"},{"key":"e_1_2_12_6_1","doi-asserted-by":"publisher","DOI":"10.1067\/moe.2003.152"},{"key":"e_1_2_12_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tripleo.2009.06.044"},{"key":"e_1_2_12_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00167\u2010010\u20101306\u2010y"},{"key":"e_1_2_12_9_1","doi-asserted-by":"publisher","DOI":"10.1902\/jop.2003.74.6.849"},{"key":"e_1_2_12_10_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00381-016-3150-y"},{"key":"e_1_2_12_11_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00381\u2010016\u20103150\u2010y"},{"key":"e_1_2_12_12_1","first-page":"1222","article-title":"Regenerative Surgery Performed With Platelet\u2010Rich Plasma Used in Sinus Lift Elevation Before Dental Implant Surgery: An Useful Aid in Healing and Regeneration of Bone Tissue","volume":"16","author":"Inchingolo F.","year":"2012","journal-title":"European Review for Medical and Pharmacological Sciences"},{"key":"e_1_2_12_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.oooo.2015.06.035"},{"key":"e_1_2_12_14_1","doi-asserted-by":"publisher","DOI":"10.1590\/0100\u201069912015006013"},{"key":"e_1_2_12_15_1","article-title":"Une opportunit\u00e9 en paro\u2010implantologie: le PRF","volume":"42","author":"Choukroun J.","year":"2001","journal-title":"Implantodontie"},{"key":"e_1_2_12_16_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00068\u2010017\u20100785\u20107"},{"key":"e_1_2_12_17_1","doi-asserted-by":"publisher","DOI":"10.3390\/ma13030622"},{"key":"e_1_2_12_18_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00784\u2010020\u201003400\u20107"},{"key":"e_1_2_12_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/jlb.65.2.137"},{"key":"e_1_2_12_20_1","first-page":"1958","article-title":"Influence of PRF in the Healing of Bone and Gingival Tissues. 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