{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,19]],"date-time":"2026-10-19T03:41:02Z","timestamp":1792381262122,"version":"4.4.0"},"reference-count":47,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2025,1,28]],"date-time":"2025-01-28T00:00:00Z","timestamp":1738022400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2025,1,28]],"date-time":"2025-01-28T00:00:00Z","timestamp":1738022400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clin Implant Dent Rel Res"],"published-print":{"date-parts":[[2025,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>This cross\u2010sectional study aimed to compare the composition of the submucosal microbiome of peri\u2010implantitis with paired and unpaired healthy implant samples.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>We evaluated submucosal plaque samples obtained in 39 cases, including 13 cases of peri\u2010implantitis, 13 cases involving healthy implants from the same patient (paired samples), and 13 cases involving healthy implants from different individuals (unpaired samples). The patients were evaluated using next\u2010generation genomic sequencing (Illumina) based on 16S rRNA gene amplification. The sequences were grouped according to the amplicon sequence variant (ASV) to define the taxonomic categories. Alpha diversity was analyzed using Shannon's and Simpson's indices, while beta diversity was evaluated using principal coordinate analysis, analysis of similarities, and permutational multivariate variance analysis. Additionally, UniFrac distances were evaluated using Quantitative Insights into Microbial Ecology 2. Finally, we evaluated between\u2010group differences in the taxonomic components.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      There were no significant between\u2010group differences in alpha diversity. The average bacterial ratios of\n                      <jats:italic>Filifactor alocis, Porphyromona endodontalis, Tannerella forsythia, Treponema denticola, Peptostreptococcaceae [Eubacterium nodatum], Desulfobulbus sp.<\/jats:italic>\n                      HTM 041, and\n                      <jats:italic>Mogibacterium timidum<\/jats:italic>\n                      significantly differed between peri\u2010implantitis samples and unpaired samples from the healthy implants (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.05). However, there were few differences in the microbiota between peri\u2010implantitis samples and those paired with healthy implants in the same patient.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Future studies comparing the microbiome compositions using sequencing techniques between healthy implants and implants with peri\u2010implantitis should focus on retrieving samples from the same patient, especially in individuals with a history of periodontitis.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/cid.13423","type":"journal-article","created":{"date-parts":[[2025,1,29]],"date-time":"2025-01-29T00:44:36Z","timestamp":1738111476000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Submucosal Microbiome Profiles in\u00a0Paired and Unpaired Samples From Healthy and Peri\u2010Implantitis Dental Implants"],"prefix":"10.1111","volume":"27","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4240-0235","authenticated-orcid":false,"given":"Oscar Iv\u00e1n","family":"Tocarruncho","sequence":"first","affiliation":[{"name":"Master Oral Science Program, School of Dentistry Universidad El Bosque  Bogot\u00e1\u2010 Colombia"},{"name":"Unit of Basic Oral Investigation\u2010UIBO, School of Dentistry Universidad El Bosque  Bogot\u00e1 Colombia"},{"name":"Instituci\u00f3n Universitaria Colegios de Colombia UNICOC School of Dentistry  Bogot\u00e1\u2010 Colombia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9350-0941","authenticated-orcid":false,"given":"Yineth","family":"Neuta","sequence":"additional","affiliation":[{"name":"Unit of Basic Oral Investigation\u2010UIBO, School of Dentistry Universidad El Bosque  Bogot\u00e1 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Colombia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2838-1015","authenticated-orcid":false,"given":"Leandro","family":"Chambrone","sequence":"additional","affiliation":[{"name":"Unit of Basic Oral Investigation\u2010UIBO, School of Dentistry Universidad El Bosque  Bogot\u00e1 Colombia"},{"name":"Evidence\u2010Based Hub, Centro de Investiga\u00e7\u00e3o Interdisciplinar Egas Moniz (CiiEM) Egas Moniz School of Health &amp; Science  Almada Portugal"},{"name":"Department of Periodontics, School of Dental Medicine The University of Pennsylvania  Philadelphia Pennsylvania USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3986-0625","authenticated-orcid":false,"given":"Gloria In\u00e9s","family":"Lafaurie","sequence":"additional","affiliation":[{"name":"Unit of Basic Oral Investigation\u2010UIBO, School of Dentistry Universidad El Bosque  Bogot\u00e1 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