{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,30]],"date-time":"2025-10-30T22:26:36Z","timestamp":1761863196415},"reference-count":38,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2013,1,7]],"date-time":"2013-01-07T00:00:00Z","timestamp":1357516800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clinical Oral Implants Res"],"published-print":{"date-parts":[[2014,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objectives<\/jats:title><jats:p>This study aimed to comparatively evaluate the <jats:italic>in vitro<\/jats:italic> osteogenic potential of cells obtained from the mandibular ramus (<jats:styled-content style=\"fixed-case\">MR<\/jats:styled-content>, autogenous bone donor site) and from the maxillary sinus (MS) bone grafted with a mixture of anorganic bovine bone (<jats:styled-content style=\"fixed-case\">ABB<\/jats:styled-content>) and <jats:styled-content style=\"fixed-case\">MR<\/jats:styled-content> prior to titanium implant placement (<jats:styled-content style=\"fixed-case\">MS<\/jats:styled-content>, grafted implant site).<\/jats:p><\/jats:sec><jats:sec><jats:title>Material and methods<\/jats:title><jats:p>Cells were obtained from three patients subjected to MS floor augmentation with a 1\u00a0:\u00a01 mixture of ABB (GenOx Inorg<jats:sup><jats:bold>\u00ae<\/jats:bold><\/jats:sup>) and MR. At the time of the sinus lift procedure and after 8\u00a0months, prior to implant placement, bone fragments were taken from MR and MS, respectively, and subjected to trypsin\u2013collagenase digestion for primary cell culturing. Subcultured cells were grown under osteogenic condition for up to 21\u00a0days and assayed for proliferation\/viability, osteoblast marker <jats:styled-content style=\"fixed-case\">mRNA<\/jats:styled-content> levels, alkaline phosphatase (ALP) activity and calcium content\/Alizarin red staining. ALP activity was also determined in primary explant cultures exposed to GenOx Inorg<jats:sup><jats:bold>\u00ae<\/jats:bold><\/jats:sup> (1\u00a0:\u00a01 with MR) for 7\u00a0days. Data were compared using either the Mann\u2013Whitney <jats:italic>U<\/jats:italic>\u2010test or the Kruskal\u2013Wallis test.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>MS cultures exhibited a significantly lower osteogenic potential compared with <jats:styled-content style=\"fixed-case\">MR<\/jats:styled-content> cultures, with a progressive increase in cell proliferation together with a decrease in osteoblast markers, reduced <jats:styled-content style=\"fixed-case\">ALP<\/jats:styled-content> activity and calcium content. Exposure of <jats:styled-content style=\"fixed-case\">MR<\/jats:styled-content>\u2010derived primary cultures to GenOx Inorg<jats:sup><jats:bold>\u00ae<\/jats:bold><\/jats:sup> inhibited <jats:styled-content style=\"fixed-case\">ALP<\/jats:styled-content> activity.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>These results suggest that the use of GenOx Inorg<jats:sup><jats:bold>\u00ae<\/jats:bold><\/jats:sup> in combination with <jats:styled-content style=\"fixed-case\">MR<\/jats:styled-content> fragments for MS floor augmentation inhibits the osteoblast cell differentiation at the implant site in the long term.<\/jats:p><\/jats:sec>","DOI":"10.1111\/clr.12100","type":"journal-article","created":{"date-parts":[[2013,1,7]],"date-time":"2013-01-07T07:26:39Z","timestamp":1357543599000},"page":"603-609","source":"Crossref","is-referenced-by-count":5,"title":["Autogenous bone combined with anorganic bovine bone for maxillary sinus augmentation: analysis of the osteogenic potential of cells derived from the donor and the grafted sites"],"prefix":"10.1111","volume":"25","author":[{"given":"Willian M.","family":"de Melo","sequence":"first","affiliation":[{"name":"School of Dentistry of Ribeir\u00e3o Preto University of S\u00e3o Paulo Ribeir\u00e3o Preto SP Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fabiola S.","family":"de Oliveira","sequence":"additional","affiliation":[{"name":"School of Dentistry of Ribeir\u00e3o Preto University of S\u00e3o Paulo Ribeir\u00e3o Preto SP Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"suffix":"Jr","given":"\u00c9lcio","family":"Marcantonio","sequence":"additional","affiliation":[{"name":"School of Dentistry of Araraquara S\u00e3o Paulo State University Araraquara SP Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marcio M.","family":"Beloti","sequence":"additional","affiliation":[{"name":"School of Dentistry of Ribeir\u00e3o Preto University 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