{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,6]],"date-time":"2026-06-06T18:05:26Z","timestamp":1780769126702,"version":"3.54.1"},"reference-count":59,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2015,4,10]],"date-time":"2015-04-10T00:00:00Z","timestamp":1428624000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001700","name":"Ministry of Education, Science, Sports, Culture, and Technology of Japan","doi-asserted-by":"crossref","award":["24592968"],"award-info":[{"award-number":["24592968"]}],"id":[{"id":"10.13039\/501100001700","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clinical Oral Implants Res"],"published-print":{"date-parts":[[2016,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objectives<\/jats:title><jats:p>The peri\u2010implant epithelium (<jats:styled-content style=\"fixed-case\">PIE<\/jats:styled-content>) plays an important role in the prevention against initial stage of inflammation. To minimize the risk of peri\u2010implantitis, it is necessary to understand the biological characteristics of the <jats:styled-content style=\"fixed-case\">PIE<\/jats:styled-content>. The aim of this study was to investigate the characteristic gene expression profile of <jats:styled-content style=\"fixed-case\">PIE<\/jats:styled-content> as compared to junctional epithelium (<jats:styled-content style=\"fixed-case\">JE<\/jats:styled-content>) using laser microdissection and microarray analysis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Left upper first molars of 4\u2010week\u2010old rat were extracted, and titanium alloy implants were placed. Four weeks after surgery, samples were harvested by laser microdissection, and total <jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> samples were isolated. Comprehensive analyses of genes expressed in the <jats:styled-content style=\"fixed-case\">JE<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">PIE<\/jats:styled-content> were performed using microarray analysis. Confirmation of the differential expression of selected genes was performed by quantitative real\u2010time polymerase chain reaction and immunohistochemistry.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The microarray analysis showed that 712 genes were more than twofold change upregulated in the <jats:styled-content style=\"fixed-case\">PIE<\/jats:styled-content> compared with the <jats:styled-content style=\"fixed-case\">JE<\/jats:styled-content>. Genes <jats:italic><jats:styled-content style=\"fixed-case\">S<\/jats:styled-content>cgb1a1<\/jats:italic> were significantly upregulated more than 19.1\u2010fold, <jats:italic><jats:styled-content style=\"fixed-case\">L<\/jats:styled-content>po<\/jats:italic> more than 19.0\u2010fold, and <jats:italic><jats:styled-content style=\"fixed-case\">G<\/jats:styled-content>bp2<\/jats:italic> more than 8.9\u2010fold, in the <jats:styled-content style=\"fixed-case\">PIE<\/jats:styled-content> (<jats:italic>P\u00a0<\/jats:italic>&lt;<jats:italic>\u00a0<\/jats:italic>0.01). Immunohistochemical localization of <jats:styled-content style=\"fixed-case\">SCGB<\/jats:styled-content>1<jats:styled-content style=\"fixed-case\">A<\/jats:styled-content>1, <jats:styled-content style=\"fixed-case\">LPO<\/jats:styled-content>, and <jats:styled-content style=\"fixed-case\">GBP<\/jats:styled-content>2 was observed in <jats:styled-content style=\"fixed-case\">PIE<\/jats:styled-content>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The present results suggested that genes <jats:italic><jats:styled-content style=\"fixed-case\">S<\/jats:styled-content>cgb1a1, <jats:styled-content style=\"fixed-case\">L<\/jats:styled-content>po,<\/jats:italic> and <jats:italic><jats:styled-content style=\"fixed-case\">G<\/jats:styled-content>bp2<\/jats:italic> are characteristically expressed in the <jats:styled-content style=\"fixed-case\">PIE<\/jats:styled-content>.<\/jats:p><\/jats:sec>","DOI":"10.1111\/clr.12601","type":"journal-article","created":{"date-parts":[[2015,4,10]],"date-time":"2015-04-10T23:05:30Z","timestamp":1428707130000},"source":"Crossref","is-referenced-by-count":13,"title":["The genes <scp>S<\/scp>cgb1a1, <scp>L<\/scp>po and <scp>G<\/scp>bp2 characteristically expressed in peri\u2010implant epithelium of rats"],"prefix":"10.1111","volume":"27","author":[{"given":"Gentaro","family":"Mori","sequence":"first","affiliation":[{"name":"Department of Oral and Maxillofacial Implantology Tokyo Dental College Tokyo Japan"},{"name":"Division of Oral Implants Research Oral Health Science Center Tokyo Dental College Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hodaka","family":"Sasaki","sequence":"additional","affiliation":[{"name":"Department of Oral and Maxillofacial Implantology Tokyo Dental College Tokyo Japan"},{"name":"Division of Oral Implants Research 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