{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T23:25:34Z","timestamp":1792797934615,"version":"4.5.0"},"reference-count":54,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2015,10,1]],"date-time":"2015-10-01T00:00:00Z","timestamp":1443657600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"University of Michigan Periodontal Graduate Student Research Fund","id":[{"id":"https:\/\/ror.org\/00jmfr291","id-type":"ROR","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clinical Oral Implants Res"],"published-print":{"date-parts":[[2016,9]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>\n                      The aim of the present investigation was to determine the profile of peri\u2010implant crevicular fluid (\n                      <jats:styled-content style=\"fixed-case\">PICF<\/jats:styled-content>\n                      ) biomarkers combined with microbial profiles from implants with healthy peri\u2010implant tissues and peri\u2010implantitis to assess real\u2010time disease activity.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Material and methods<\/jats:title>\n                    <jats:p>\n                      Sixty\u2010eight patients were included in this cross\u2010sectional study. They were divided into two groups: 34 patients with at least one healthy implant (control) and 34 with at least one peri\u2010implantitis affected implant (test). Total\n                      <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content>\n                      content and\n                      <jats:styled-content style=\"fixed-case\">qPCR<\/jats:styled-content>\n                      analysis for periodontal bacteria obtained from subgingival plaque samples (\n                      <jats:italic>Aggregatibacter actinomycetemcomitans, Prevotella intermedia, Porphyromonas gingivalis, Tannerella forsythia,<\/jats:italic>\n                      and\n                      <jats:italic>Treponema denticola<\/jats:italic>\n                      ) and a\n                      <jats:styled-content style=\"fixed-case\">PICF<\/jats:styled-content>\n                      analysis for\n                      <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\n                      \u20101\u03b2,\n                      <jats:styled-content style=\"fixed-case\">VEGF<\/jats:styled-content>\n                      ,\n                      <jats:styled-content style=\"fixed-case\">MMP<\/jats:styled-content>\n                      \u20108,\n                      <jats:styled-content style=\"fixed-case\">TIMP<\/jats:styled-content>\n                      \u20102, and\n                      <jats:styled-content style=\"fixed-case\">OPG<\/jats:styled-content>\n                      were performed. The individual and combined diagnostic ability of each biomarker for peri\u2010implantitis and target bacterial species were analyzed.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      The mean concentration of\n                      <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\n                      \u20101\u03b2 (44.6 vs. 135.8\u00a0pg\/ml;\n                      <jats:italic>P<\/jats:italic>\n                      \u00a0&lt;\u00a00.001),\n                      <jats:styled-content style=\"fixed-case\">TIMP<\/jats:styled-content>\n                      \u20102 (5488.3 vs. 9771.8\u00a0pg\/ml;\n                      <jats:italic>P<\/jats:italic>\n                      \u00a0=\u00a00.001),\n                      <jats:styled-content style=\"fixed-case\">VEGF<\/jats:styled-content>\n                      (59.1 vs. 129.0\u00a0pg\/ml;\n                      <jats:italic>P<\/jats:italic>\n                      \u00a0=\u00a00.012), and\n                      <jats:styled-content style=\"fixed-case\">OPG<\/jats:styled-content>\n                      (66.5 vs. 111.7\u00a0pg\/ml;\n                      <jats:italic>P<\/jats:italic>\n                      \u00a0=\u00a00.050) was increased in the peri\u2010implantitis patients. The mean expression of\n                      <jats:styled-content style=\"fixed-case\">MMP<\/jats:styled-content>\n                      \u20108 (6029.2 vs. 5943.1\u00a0pg\/ml;\n                      <jats:italic>P<\/jats:italic>\n                      \u00a0=\u00a00.454) and did not reveal a meaningful difference among groups. Total bacterial\n                      <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content>\n                      of selected microorganisms was associated with a threefold or greater increase in peri\u2010implantitis although no statistical significant difference. The ability to diagnose diseased sites was enhanced by\n                      <jats:italic>T.\u00a0denticola<\/jats:italic>\n                      combined with\n                      <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\n                      \u20101\u03b2,\n                      <jats:styled-content style=\"fixed-case\">VEGF<\/jats:styled-content>\n                      , and\n                      <jats:styled-content style=\"fixed-case\">TIMP<\/jats:styled-content>\n                      \u20102\n                      <jats:styled-content style=\"fixed-case\">PICF<\/jats:styled-content>\n                      levels.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>\n                      The present data suggest that the increased levels of the selected\n                      <jats:styled-content style=\"fixed-case\">PICF<\/jats:styled-content>\n                      \u2010derived biomarkers of periodontal tissue inflammation, matrix degradation\/regulation, and alveolar bone turnover\/resorption combined with site\u2010specific microbial profiles may be associated with peri\u2010implantitis and could have potential as predictors of peri\u2010implant diseases.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/clr.12708","type":"journal-article","created":{"date-parts":[[2015,10,1]],"date-time":"2015-10-01T01:24:46Z","timestamp":1443662686000},"page":"1129-1136","source":"Crossref","is-referenced-by-count":100,"title":["Protein biomarkers and microbial profiles in peri\u2010implantitis"],"prefix":"10.1111","volume":"27","author":[{"given":"Hom\u2010Lay","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Periodontics and Oral Medicine University of Michigan School of Dentistry Ann Arbor MI 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