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This abundant fishing by\u2010product provides an improved hydroxyapatite (<jats:styled-content style=\"fixed-case\">HA<\/jats:styled-content>) with fluorine contributions. The<jats:italic>in\u00a0vivo<\/jats:italic>evaluation of osteointegration and bone mineral density levels promoted by these marine bioapatites was the main objective.<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and Methods<\/jats:title><jats:p>Marine bioapatite granules of two sizes (1\u00a0mm, &lt;20\u00a0\u03bcm) were obtained and characterized (<jats:styled-content style=\"fixed-case\">XRD<\/jats:styled-content>,<jats:styled-content style=\"fixed-case\">SEM<\/jats:styled-content>,<jats:styled-content style=\"fixed-case\">ICP<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">OES<\/jats:styled-content>) to determine morphology and composition.<jats:italic>In vivo<\/jats:italic>evaluation was performed, after bioapatites implantation in critical defects of parietal bone of 25 rats, for 3\u00a0weeks. Commercial synthetic<jats:styled-content style=\"fixed-case\">HA<\/jats:styled-content>\/\u03b2<jats:styled-content style=\"fixed-case\">TCP<\/jats:styled-content>(60\/40%) material and unfilled defects were used as controls. Radiology, micro\u2010<jats:styled-content style=\"fixed-case\">CT<\/jats:styled-content>, histology and quantification of bone mineral density are presented.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>These marine bioapatites presented a globular porous morphology. A biphasic composition ~70% apatitic (<jats:styled-content style=\"fixed-case\">HA<\/jats:styled-content>, apatite\u2010CaF, fluorapatite) and ~30% non\u2010apatitic phase (whitlockite, tricalcium bis(orthophosphate)), with contributions of F (1.0\u00a0\u00b1\u00a00.5%wt), Na (0.9\u00a0\u00b1\u00a00.2%wt) and Mg (0.65\u00a0\u00b1\u00a00.04%wt) was confirmed. After implantation period, higher osteointegration of 1\u2010mm marine bioapatites than commercial synthetic granules was observed, together with bone formation from the defect surroundings but also at central area (potential osteoinductive properties). New bone cells penetrated inside pores and inter\u2010granular cavities. Higher bone mineral density, in both 1\u2010mm and &lt;20\u2010\u03bcm granules, than on commercial synthetic graft was determined, being significant in 1\u2010mm bioapatites (a<jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00.05).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Shark teeth bioapatites were successfully validated as new functionally efficient bone filler in rat model, promoting significantly increased bone mineral density than synthetic control.<\/jats:p><\/jats:sec>","DOI":"10.1111\/clr.12934","type":"journal-article","created":{"date-parts":[[2016,8,5]],"date-time":"2016-08-05T11:54:02Z","timestamp":1470398042000},"source":"Crossref","is-referenced-by-count":16,"title":["<i>In vivo<\/i>evaluation of shark teeth\u2010derived bioapatites"],"prefix":"10.1111","volume":"28","author":[{"given":"Miriam","family":"L\u00f3pez\u2010\u00c1lvarez","sequence":"first","affiliation":[{"name":"New Materials Group Applied Physics Department Institute of 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