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PICF samples were collected and analyzed using both non\u2010targeted and targeted metabolomics approaches. The relationship between metabolites and clinical indices including probing depth (PD), bleeding on probing (BOP), and marginal bone loss (MBL) was examined. Additionally, submucosal microbiota was collected and analyzed using 16S rRNA gene sequencing to elucidate the association between the metabolites and microbial communities.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Significant differences in metabolic profiles were observed between the PH and PI groups, with 179 distinct metabolites identified. In the PI group, specific amino acids and fatty acids were significantly elevated compared to the PH group. Organic acids including succinic acid, fructose\u20106\u2010phosphate, and glucose\u20106\u2010phosphate were markedly higher in the PI group, showing positive correlations with mean PD, BOP, and MBL. Metabolites that increased in the PI group positively correlated with the presence of <jats:italic>Porphyromonas<\/jats:italic> and <jats:italic>Treponema<\/jats:italic> and negatively with <jats:italic>Streptococcus<\/jats:italic> and <jats:italic>Haemophilus<\/jats:italic>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>This study establishes a clear association between metabolic compositions and peri\u2010implant condition, highlighting enhanced metabolite activity in peri\u2010implantitis. These findings open avenues for further research into metabolic mechanisms of peri\u2010implantitis and their potential therapeutic implications.<\/jats:p><\/jats:sec>","DOI":"10.1111\/clr.14270","type":"journal-article","created":{"date-parts":[[2024,4,16]],"date-time":"2024-04-16T13:35:40Z","timestamp":1713274540000},"page":"719-728","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Metabolic profiling of peri\u2010implant crevicular fluid in peri\u2010implantitis"],"prefix":"10.1111","volume":"35","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9019-2004","authenticated-orcid":false,"given":"Lu","family":"Song","sequence":"first","affiliation":[{"name":"School of Stomatology, Zhejiang University School of Medicine, Zhejiang Provincial Clinical Research Center for Oral Diseases, Key Laboratory of Oral Biomedical Research of Zhejiang Province, Stomatology Hospital Cancer Center of Zhejiang University  Hangzhou 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