{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T18:11:21Z","timestamp":1789495881448,"version":"build-2803163510"},"reference-count":46,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2024,11,22]],"date-time":"2024-11-22T00:00:00Z","timestamp":1732233600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clinical Oral Implants Res"],"published-print":{"date-parts":[[2025,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:sec><jats:title>Objective<\/jats:title><jats:p>The objective of this study is to compare the effect of the location of recombinant human bone morphogenetic protein 2 (rhBMP\u20102) from the native bone and the periosteum for vertical alveolar bone augmentation.<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and Methods<\/jats:title><jats:p>Mandibular, chronic, standardized, bilateral, and vertical defects in 12 beagle dogs were evaluated using four modalities: a xenograft alone (XENO; <jats:italic>n<\/jats:italic>\u2009=\u20096); rhBMP\u20102 alone (BMP; <jats:italic>n<\/jats:italic>\u2009=\u20096); a technique with rhBMP\u20102 close to the host bone covered by xenograft (SAN; <jats:italic>n<\/jats:italic>\u2009=\u20096); and a technique with rhBMP\u20102 close to the flap on top of the xenograft (LAS; <jats:italic>n<\/jats:italic>\u2009=\u20096). After 8\u2009weeks, a series of in\u00a0vivo inspections, fluorescence microscopy, histologic and histomorphometric evaluations, and micro\u2010CT analyses.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>After 8\u2009weeks of healing, new bone formation correlated with proximity of rhBMP to the perforated membrane with BMP and LAS (<jats:italic>p<\/jats:italic>\u2009=\u20090.024). The highest total bone volume was found in the LAS group (45.1%\u2009\u00b1\u200913.3%), followed by the SAN group (35.2%\u2009\u00b1\u20096.7%), BMP group (33.1%\u2009\u00b1\u200911.8%), followed by the XENO group (23.1%\u2009\u00b1\u20096.5%). The SAN group demonstrated frequent seroma formation. Blood vessel formation was more pronounced in the LAS\u2009+\u2009rhBMP group, with a significant increase of 27.1% compared to the XENO group (<jats:italic>p<\/jats:italic>\u2009=\u20090.02). Micro\u2010CT revealed a strong trend for higher bone volume in the BMP group (34.7%) compared to the XENO group (13.6%) (<jats:italic>p<\/jats:italic>\u2009=\u20090.06). Only rhBMP\u20102 groups demonstrated bone formation above the perforated membrane.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The location of rhBMP\u20102 in relation to the biomaterial and periosteum influenced the effectiveness of vertical bone regeneration.<\/jats:p><\/jats:sec>","DOI":"10.1111\/clr.14379","type":"journal-article","created":{"date-parts":[[2024,11,22]],"date-time":"2024-11-22T13:14:13Z","timestamp":1732281253000},"page":"250-264","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Regeneration of Chronic Alveolar Vertical Defects Using a Micro Dosage of rhBMP\u20102. An Experimental In\u00a0Vivo Study"],"prefix":"10.1111","volume":"36","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9368-4047","authenticated-orcid":false,"given":"Istvan A.","family":"Urban","sequence":"first","affiliation":[{"name":"Department of Oral Medicine, Infection Control and Immunity Harvard University  Boston Massachusetts USA"},{"name":"Department of Periodontics and Oral Medicine University of Michigan School of Dentistry  Ann Arbor Michigan USA"},{"name":"Urban Regeneration Institute  Budapest Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7531-8046","authenticated-orcid":false,"given":"S\u00e1ndor","family":"Farkasdi","sequence":"additional","affiliation":[{"name":"Department of Oral Biology Semmelweis University  Budapest Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dieter D.","family":"Bosshardt","sequence":"additional","affiliation":[{"name":"Department of Periodontology, School of Dental Medicine University of Bern  Bern Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2224-982X","authenticated-orcid":false,"given":"Mauricio G.","family":"Araujo","sequence":"additional","affiliation":[{"name":"State University of Maring\u00e1  Maring\u00e1 Paran\u00e1 Brazil"},{"name":"Private Practice in Dental Implants and Periodontics  Rio de Janeiro Rio de Janeiro Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3029-8130","authenticated-orcid":false,"given":"Andrea","family":"Ravid\u00e0","sequence":"additional","affiliation":[{"name":"Department of Periodontics &amp; Oral Medicine University of Pittsburg  Pittsburgh Pennsylvania USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1936-4683","authenticated-orcid":false,"given":"Kathrin","family":"Becker","sequence":"additional","affiliation":[{"name":"Department of Orthodontics and Dentofacial Orthopedics, Center for Oral Health Sciences Charit\u00e9\u2010Universit\u00e4tsmedizin  Berlin Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-1167-711X","authenticated-orcid":false,"given":"Robert","family":"Kerberger","sequence":"additional","affiliation":[{"name":"Department of Orthodontics and Dentofacial Orthopedics, Center for Oral Health Sciences Charit\u00e9\u2010Universit\u00e4tsmedizin  Berlin Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4238-1799","authenticated-orcid":false,"given":"Hom\u2010Lay","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Periodontics and Oral Medicine University of Michigan School of Dentistry  Ann Arbor Michigan USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1607-0583","authenticated-orcid":false,"given":"Ulf M. 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