{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T16:33:41Z","timestamp":1791304421722,"version":"4.3.1"},"reference-count":49,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2015,9,1]],"date-time":"2015-09-01T00:00:00Z","timestamp":1441065600000},"content-version":"tdm","delay-in-days":510,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"name":"Grant-in-Aid for Scientific Research C from the Japanese Ministry of Education, Culture, Sports and Technology in Japan","award":["24590528"],"award-info":[{"award-number":["24590528"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cell Microbiol"],"published-print":{"date-parts":[[2014,9]]},"DOI":"10.1111\/cmi.12296","type":"journal-article","created":{"date-parts":[[2014,3,21]],"date-time":"2014-03-21T05:56:51Z","timestamp":1395381411000},"page":"1366-1377","source":"Crossref","is-referenced-by-count":38,"title":["Leptospiral outer membrane protein LMB216 is involved in enhancement of phagocytic uptake by macrophages"],"prefix":"10.1155","volume":"16","author":[{"given":"Claudia","family":"Toma","sequence":"first","affiliation":[{"name":"Department of Molecular Bacteriology and Immunology; Graduate School of Medicine; University of the Ryukyus; Okinawa 903-0215 Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gerald L.","family":"Murray","sequence":"additional","affiliation":[{"name":"Australian Research Council Centre of Excellence in Structural and Functional Microbial Genomics; Department of Microbiology; Monash University; Clayton Vic. 3800 Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Toshitsugu","family":"Nohara","sequence":"additional","affiliation":[{"name":"Department of Molecular Bacteriology and Immunology; Graduate School of Medicine; University of the Ryukyus; Okinawa 903-0215 Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masaru","family":"Mizuyama","sequence":"additional","affiliation":[{"name":"Department of Molecular Bacteriology and Immunology; Graduate School of Medicine; University of the Ryukyus; Okinawa 903-0215 Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nobuo","family":"Koizumi","sequence":"additional","affiliation":[{"name":"Department of Bacteriology I; National Institute of Infectious Diseases; Tokyo 162-8640 Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ben","family":"Adler","sequence":"additional","affiliation":[{"name":"Australian Research Council Centre of Excellence in Structural and Functional Microbial Genomics; Department of Microbiology; Monash University; Clayton Vic. 3800 Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Toshihiko","family":"Suzuki","sequence":"additional","affiliation":[{"name":"Department of Molecular Bacteriology and Immunology; Graduate School of Medicine; University of the Ryukyus; Okinawa 903-0215 Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2014,4,9]]},"reference":[{"key":"10.1111\/cmi.12296-BIB0001|cmi12296-cit-0001","doi-asserted-by":"crossref","DOI":"10.1016\/j.vetmic.2011.02.055","article-title":"Pathogenesis of leptospirosis: the influence of genomics","author":"Adler","year":"2011","journal-title":"Vet Microbiol"},{"key":"10.1111\/cmi.12296-BIB0002|cmi12296-cit-0002","doi-asserted-by":"crossref","first-page":"70","DOI":"10.1186\/1471-2180-8-70","article-title":"Lsa21, a novel leptospiral protein binding adhesive matrix molecules and present during human infection","volume":"8","author":"Atzingen","year":"2008","journal-title":"BMC Microbiol"},{"key":"10.1111\/cmi.12296-BIB0003|cmi12296-cit-0003","doi-asserted-by":"crossref","first-page":"264","DOI":"10.1016\/j.jinf.2009.07.010","article-title":"Lp95, a novel leptospiral protein that binds extracellular matrix components and activates e-selectin on endothelial cells","volume":"59","author":"Atzingen","year":"2009","journal-title":"J Infect"},{"key":"10.1111\/cmi.12296-BIB0004|cmi12296-cit-0004","doi-asserted-by":"crossref","first-page":"185","DOI":"10.1093\/bioinformatics\/19.2.185","article-title":"A comparison of normalization methods for high density oligonucleotide array data based on variance and bias","volume":"19","author":"Bolstad","year":"2003","journal-title":"Bioinformatics"},{"key":"10.1111\/cmi.12296-BIB0005|cmi12296-cit-0005","doi-asserted-by":"crossref","first-page":"3255","DOI":"10.1128\/JB.187.9.3255-3258.2005","article-title":"Random insertional mutagenesis of Leptospira interrogans, the agent of leptospirosis, using a mariner transposon","volume":"187","author":"Bourhy","year":"2005","journal-title":"J Bacteriol"},{"key":"10.1111\/cmi.12296-BIB0006|cmi12296-cit-0006","doi-asserted-by":"crossref","first-page":"995","DOI":"10.1093\/infdis\/jir875","article-title":"Leptospiral immunoglobulin-like proteins interact with human complement regulators factor H, FHL-1, FHR-1, and C4BP","volume":"205","author":"Castiblanco-Valencia","year":"2012","journal-title":"J Infect Dis"},{"key":"10.1111\/cmi.12296-BIB0007|cmi12296-cit-0007","doi-asserted-by":"crossref","first-page":"2669","DOI":"10.4049\/jimmunol.0900506","article-title":"TLR4- and TLR2-mediated B cell responses control the clearance of the bacterial pathogen, Leptospira interrogans","volume":"183","author":"Chassin","year":"2009","journal-title":"J Immunol"},{"key":"10.1111\/cmi.12296-BIB0008|cmi12296-cit-0008","doi-asserted-by":"crossref","first-page":"2441","DOI":"10.1128\/IAI.01635-06","article-title":"Physiological osmotic induction of Leptospira interrogans adhesion: LigA and LigB bind extracellular matrix proteins and fibrinogen","volume":"75","author":"Choy","year":"2007","journal-title":"Infect Immun"},{"key":"10.1111\/cmi.12296-BIB0009|cmi12296-cit-0009","doi-asserted-by":"crossref","first-page":"e16879","DOI":"10.1371\/journal.pone.0016879","article-title":"The multifunctional LigB adhesin binds homeostatic proteins with potential roles in cutaneous infection by pathogenic Leptospira interrogans","volume":"6","author":"Choy","year":"2011","journal-title":"PLoS ONE"},{"key":"10.1111\/cmi.12296-BIB0010|cmi12296-cit-0010","doi-asserted-by":"crossref","first-page":"5826","DOI":"10.1128\/IAI.00989-08","article-title":"Targeted mutagenesis in pathogenic Leptospira species: disruption of the LigB gene does not affect virulence in animal models of leptospirosis","volume":"76","author":"Croda","year":"2008","journal-title":"Infect Immun"},{"key":"10.1111\/cmi.12296-BIB9010|cmi12296-cit-9010","doi-asserted-by":"crossref","first-page":"3892","DOI":"10.1128\/IAI.00466-12","article-title":"Leptospira interrogans catalase is required for resistance to H2O2 and for virulence","volume":"80","author":"Eshghi","year":"2012","journal-title":"Infect Immun"},{"key":"10.1111\/cmi.12296-BIB0011|cmi12296-cit-0011","doi-asserted-by":"crossref","first-page":"129","DOI":"10.1186\/1471-2180-11-129","article-title":"Heterologous expression of pathogen-specific genes ligA and ligB in the saprophyte Leptospira biflexa confers enhanced adhesion to cultured cells and fibronectin","volume":"11","author":"Figueira","year":"2011","journal-title":"BMC Microbiol"},{"key":"10.1111\/cmi.12296-BIB0012|cmi12296-cit-0012","doi-asserted-by":"crossref","first-page":"1491","DOI":"10.1099\/00221287-146-7-1491","article-title":"Spirochaetal lipoproteins and pathogenesis","volume":"146","author":"Haake","year":"2000","journal-title":"Microbiology"},{"key":"10.1111\/cmi.12296-BIB0013|cmi12296-cit-0013","doi-asserted-by":"crossref","first-page":"1337","DOI":"10.1007\/s00284-010-9865-1","article-title":"Characterization of the immunogenic and antigenic potential of putative lipoproteins from Leptospira interrogans","volume":"62","author":"Hartwig","year":"2011","journal-title":"Curr Microbiol"},{"key":"10.1111\/cmi.12296-BIB0014|cmi12296-cit-0014","doi-asserted-by":"crossref","first-page":"942","DOI":"10.1172\/JCI114796","article-title":"Neutrophil bactericidal activity against Staphylococcus aureus adherent on biological surfaces. Surface-bound extracellular matrix proteins activate intracellular killing by oxygen-dependent and -independent mechanisms","volume":"86","author":"Hermann","year":"1990","journal-title":"J Clin Invest"},{"key":"10.1111\/cmi.12296-BIB0015|cmi12296-cit-0015","doi-asserted-by":"crossref","first-page":"249","DOI":"10.1093\/biostatistics\/4.2.249","article-title":"Exploration, normalization, and summaries of high density oligonucleotide array probe level data","volume":"4","author":"Irizarry","year":"2003","journal-title":"Biostatistics"},{"key":"10.1111\/cmi.12296-BIB0016|cmi12296-cit-0016","doi-asserted-by":"crossref","first-page":"1161","DOI":"10.1099\/mic.0.047639-0","article-title":"UafB is a serine-rich repeat adhesin of Staphylococcus saprophyticus that mediates binding to fibronectin, fibrinogen and human uroepithelial cells","volume":"157","author":"King","year":"2011","journal-title":"Microbiology"},{"key":"10.1111\/cmi.12296-BIB0017|cmi12296-cit-0017","doi-asserted-by":"crossref","first-page":"736","DOI":"10.1038\/nrmicro2208","article-title":"Leptospira: the dawn of the molecular genetics era for an emerging zoonotic pathogen","volume":"7","author":"Ko","year":"2009","journal-title":"Nat Rev Microbiol"},{"key":"10.1111\/cmi.12296-BIB0018|cmi12296-cit-0018","doi-asserted-by":"crossref","first-page":"1545","DOI":"10.1016\/j.vaccine.2003.10.007","article-title":"Leptospiral immunoglobulin-like proteins elicit protective immunity","volume":"22","author":"Koizumi","year":"2004","journal-title":"Vaccine"},{"key":"10.1111\/cmi.12296-BIB0019|cmi12296-cit-0019","doi-asserted-by":"crossref","first-page":"1223","DOI":"10.1038\/sj.emboj.7600166","article-title":"Structure of a thrombospondin C-terminal fragment reveals a novel calcium core in the type 3 repeats","volume":"23","author":"Kvansakul","year":"2004","journal-title":"EMBO J"},{"key":"10.1111\/cmi.12296-BIB0020|cmi12296-cit-0020","doi-asserted-by":"crossref","first-page":"2019","DOI":"10.1128\/IAI.00131-12","article-title":"FlaA proteins in Leptospira interrogans are essential for motility and virulence but are not required for formation of the flagellum sheath","volume":"80","author":"Lambert","year":"2012","journal-title":"Infect Immun"},{"key":"10.1111\/cmi.12296-BIB0021|cmi12296-cit-0021","doi-asserted-by":"crossref","first-page":"e2468","DOI":"10.1371\/journal.pntd.0002468","article-title":"Pathogenomic inference of virulence-associated genes in Leptospira interrogans","volume":"7","author":"Lehmann","year":"2013","journal-title":"PLoS Negl Trop Dis"},{"key":"10.1111\/cmi.12296-BIB0022|cmi12296-cit-0022","doi-asserted-by":"crossref","first-page":"591","DOI":"10.1007\/s00203-007-0280-3","article-title":"Comparison of invasion of fibroblasts and macrophages by high- and low-virulence Leptospira strains: colonization of the host-cell nucleus and induction of necrosis by the virulent strain","volume":"188","author":"Li","year":"2007","journal-title":"Arch Microbiol"},{"key":"10.1111\/cmi.12296-BIB0023|cmi12296-cit-0023","doi-asserted-by":"crossref","first-page":"80","DOI":"10.1177\/1753425909105580","article-title":"Replication or death: distinct fates of pathogenic Leptospira strain Lai within macrophages of human or mouse origin","volume":"16","author":"Li","year":"2010","journal-title":"Innate Immun"},{"key":"10.1111\/cmi.12296-BIB0024|cmi12296-cit-0024","doi-asserted-by":"crossref","first-page":"253","DOI":"10.1186\/1471-2180-9-253","article-title":"Inactivation of the fliY gene encoding a flagellar motor switch protein attenuates mobility and virulence of Leptospira interrogans strain Lai","volume":"9","author":"Liao","year":"2009","journal-title":"BMC Microbiol"},{"key":"10.1111\/cmi.12296-BIB0025|cmi12296-cit-0025","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1016\/j.bbrc.2009.08.089","article-title":"A novel fibronectin type III module binding motif identified on C-terminus of Leptospira immunoglobulin-like protein, LigB","volume":"389","author":"Lin","year":"2009","journal-title":"Biochem Biophys Res Commun"},{"key":"10.1111\/cmi.12296-BIB0026|cmi12296-cit-0026","doi-asserted-by":"crossref","first-page":"e11301","DOI":"10.1371\/journal.pone.0011301","article-title":"The terminal immunoglobulin-like repeats of LigA and LigB of Leptospira enhance their binding to gelatin binding domain of fibronectin and host cells","volume":"5","author":"Lin","year":"2010","journal-title":"PLoS ONE"},{"key":"10.1111\/cmi.12296-BIB0027|cmi12296-cit-0027","doi-asserted-by":"crossref","first-page":"5848","DOI":"10.1128\/IAI.00755-06","article-title":"Effects of temperature on gene expression patterns in Leptospira interrogans serovar Lai as assessed by whole-genome microarrays","volume":"74","author":"Lo","year":"2006","journal-title":"Infect Immun"},{"key":"10.1111\/cmi.12296-BIB0028|cmi12296-cit-0028","doi-asserted-by":"crossref","first-page":"929","DOI":"10.1046\/j.1365-2958.2003.03619.x","article-title":"Pathogenic Leptospira species express surface-exposed proteins belonging to the bacterial immunoglobulin superfamily","volume":"49","author":"Matsunaga","year":"2003","journal-title":"Mol Microbiol"},{"key":"10.1111\/cmi.12296-BIB0029|cmi12296-cit-0029","doi-asserted-by":"crossref","first-page":"729","DOI":"10.1128\/IAI.65.2.729-738.1997","article-title":"Invasion of Vero cells and induction of apoptosis in macrophages by pathogenic Leptospira interrogans are correlated with virulence","volume":"65","author":"Merien","year":"1997","journal-title":"Infect Immun"},{"key":"10.1111\/cmi.12296-BIB0030|cmi12296-cit-0030","doi-asserted-by":"crossref","first-page":"1116","DOI":"10.1111\/mmi.12045","article-title":"Vascular binding of a pathogen under shear force through mechanistically distinct sequential interactions with host macromolecules","volume":"86","author":"Moriarty","year":"2012","journal-title":"Mol Microbiol"},{"key":"10.1111\/cmi.12296-BIB0031|cmi12296-cit-0031","doi-asserted-by":"crossref","first-page":"305","DOI":"10.1016\/j.vetmic.2012.11.005","article-title":"The lipoprotein LipL32, an enigma of leptospiral biology","volume":"162","author":"Murray","year":"2013","journal-title":"Vet Microbiol"},{"key":"10.1111\/cmi.12296-BIB0032|cmi12296-cit-0032","doi-asserted-by":"crossref","first-page":"791","DOI":"10.1016\/j.micinf.2008.04.010","article-title":"Leptospira interrogans requires a functional heme oxygenase to scavenge iron from hemoglobin","volume":"10","author":"Murray","year":"2008","journal-title":"Microbes Infect"},{"key":"10.1111\/cmi.12296-BIB0033|cmi12296-cit-0033","doi-asserted-by":"crossref","first-page":"810","DOI":"10.1128\/IAI.01293-08","article-title":"Genome-wide transposon mutagenesis in pathogenic Leptospira species","volume":"77","author":"Murray","year":"2009a","journal-title":"Infect Immun"},{"key":"10.1111\/cmi.12296-BIB0034|cmi12296-cit-0034","doi-asserted-by":"crossref","first-page":"952","DOI":"10.1128\/IAI.01370-08","article-title":"Major surface protein LipL32 is not required for either acute or chronic infection with Leptospira interrogans","volume":"77","author":"Murray","year":"2009b","journal-title":"Infect Immun"},{"key":"10.1111\/cmi.12296-BIB0035|cmi12296-cit-0035","doi-asserted-by":"crossref","first-page":"701","DOI":"10.1111\/j.1365-2958.2010.07360.x","article-title":"Mutations affecting Leptospira interrogans lipopolysaccharide attenuate virulence","volume":"78","author":"Murray","year":"2010","journal-title":"Mol Microbiol"},{"key":"10.1111\/cmi.12296-BIB0036|cmi12296-cit-0036","doi-asserted-by":"crossref","first-page":"5924","DOI":"10.1128\/IAI.70.11.5924-5930.2002","article-title":"Cloning and molecular characterization of an immunogenic LigA protein of Leptospira interrogans","volume":"70","author":"Palaniappan","year":"2002","journal-title":"Infect Immun"},{"issue":"53","key":"10.1111\/cmi.12296-BIB0037|cmi12296-cit-0037","first-page":"pii: 2805","article-title":"Immuno-fluorescence assay of leptospiral surface-exposed proteins","author":"Pinne","year":"2011","journal-title":"J Vis Exp"},{"key":"10.1111\/cmi.12296-BIB0038|cmi12296-cit-0038","doi-asserted-by":"crossref","first-page":"888","DOI":"10.1038\/nature01597","article-title":"Unique physiological and pathogenic features of Leptospira interrogans revealed by whole-genome sequencing","volume":"422","author":"Ren","year":"2003","journal-title":"Nature"},{"key":"10.1111\/cmi.12296-BIB9011|cmi12296-cit-9011","doi-asserted-by":"crossref","first-page":"e97","DOI":"10.1371\/journal.ppat.0030097","article-title":"The OmpA-like protein Loa22 is essential for leptospiral virulence","volume":"3","author":"Ristow","year":"2007","journal-title":"PLoS Pathog"},{"key":"10.1111\/cmi.12296-BIB0039|cmi12296-cit-0039","doi-asserted-by":"crossref","first-page":"113","DOI":"10.1099\/mic.0.28317-0","article-title":"Lipoprotein computational prediction in spirochaetal genomes","volume":"152","author":"Setubal","year":"2006","journal-title":"Microbiology"},{"key":"10.1111\/cmi.12296-BIB0040|cmi12296-cit-0040","doi-asserted-by":"crossref","first-page":"2215","DOI":"10.1128\/IAI.00133-11","article-title":"Role of fibronectin-binding proteins A and B in in vitro cellular infections and in vivo septic infections by Staphylococcus aureus","volume":"79","author":"Shinji","year":"2011","journal-title":"Infect Immun"},{"key":"10.1111\/cmi.12296-BIB0041|cmi12296-cit-0041","doi-asserted-by":"crossref","first-page":"e1188","DOI":"10.1371\/journal.pone.0001188","article-title":"Leptospira interrogans endostatin-like outer membrane proteins bind host fibronectin, laminin and regulators of complement","volume":"2","author":"Stevenson","year":"2007","journal-title":"PLoS ONE"},{"key":"10.1111\/cmi.12296-BIB0042|cmi12296-cit-0042","doi-asserted-by":"crossref","first-page":"2669","DOI":"10.1128\/JCM.41.6.2669-2671.2003","article-title":"Multiplex PCR assay for identification of human diarrheagenic Escherichia coli","volume":"41","author":"Toma","year":"2003","journal-title":"J Clin Microbiol"},{"key":"10.1111\/cmi.12296-BIB0043|cmi12296-cit-0043","doi-asserted-by":"crossref","first-page":"1783","DOI":"10.1111\/j.1462-5822.2011.01660.x","article-title":"Characteristic features of intracellular pathogenic Leptospira in infected murine macrophages","volume":"13","author":"Toma","year":"2011","journal-title":"Cell Microbiol"},{"key":"10.1111\/cmi.12296-BIB0044|cmi12296-cit-0044","doi-asserted-by":"crossref","first-page":"452","DOI":"10.1128\/CVI.05524-11","article-title":"Antibodies to a novel leptospiral protein, LruC, in the eye fluids and sera of horses with Leptospira-associated uveitis","volume":"19","author":"Verma","year":"2012","journal-title":"Clin Vaccine Immunol"},{"key":"10.1111\/cmi.12296-BIB0045|cmi12296-cit-0045","doi-asserted-by":"crossref","first-page":"887","DOI":"10.1128\/IAI.74.2.887-895.2006","article-title":"Toll-like receptor 4 protects against lethal Leptospira interrogans serovar icterohaemorrhagiae infection and contributes to in vivo control of leptospiral burden","volume":"74","author":"Viriyakosol","year":"2006","journal-title":"Infect Immun"},{"key":"10.1111\/cmi.12296-BIB0046|cmi12296-cit-0046","doi-asserted-by":"crossref","first-page":"1006","DOI":"10.1111\/j.1365-2958.2012.07985.x","article-title":"The mammalian cell entry (Mce) protein of pathogenic Leptospira species is responsible for RGD motif-dependent infection of cells and animals","volume":"83","author":"Zhang","year":"2012","journal-title":"Mol Microbiol"},{"key":"10.1111\/cmi.12296-BIB0047|cmi12296-cit-0047","doi-asserted-by":"crossref","first-page":"1210","DOI":"10.1038\/cr.2011.46","article-title":"Comparative proteogenomic analysis of the Leptospira interrogans virulence-attenuated strain IPAV against the pathogenic strain 56601","volume":"21","author":"Zhong","year":"2011","journal-title":"Cell Res"}],"container-title":["Cellular Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fcmi.12296","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/onlinelibrary.wiley.com\/wol1\/doi\/10.1111\/cmi.12296\/fullpdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,1,28]],"date-time":"2022-01-28T01:00:00Z","timestamp":1643331600000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/cmi.12296"}},"subtitle":["Leptospiral OMP LMB216 enhances phagocytic uptake"],"short-title":[],"issued":{"date-parts":[[2014,4,9]]},"references-count":49,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2014,9]]}},"URL":"https:\/\/doi.org\/10.1111\/cmi.12296","relation":{},"ISSN":["1462-5814"],"issn-type":[{"value":"1462-5814","type":"print"}],"subject":[],"published":{"date-parts":[[2014,4,9]]}}}