{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T06:37:26Z","timestamp":1790145446528,"version":"4.0.1"},"reference-count":48,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2014,9,24]],"date-time":"2014-09-24T00:00:00Z","timestamp":1411516800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2014,9,24]],"date-time":"2014-09-24T00:00:00Z","timestamp":1411516800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100000268","name":"BBSRC","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100000268","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100000865","name":"Bill and Melinda Gates Foundation","doi-asserted-by":"crossref","id":[{"id":"10.13039\/100000865","id-type":"DOI","asserted-by":"crossref"}]},{"name":"EVIMalaR"},{"DOI":"10.13039\/501100004167","name":"Medicines for Malaria Venture","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100004167","id-type":"DOI","asserted-by":"crossref"}]},{"name":"TransmalariaBloc"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cellular Microbiology"],"published-print":{"date-parts":[[2015,2]]},"abstract":"Summary<\/jats:title>\n \n Gametocytes are the sole\n \n P<\/jats:italic>\n <\/jats:styled-content>\n lasmodium<\/jats:italic>\n parasite stages that infect mosquitoes; therefore development of functional gametes is required for malaria transmission. Flagellum assembly of the\n \n P<\/jats:italic>\n <\/jats:styled-content>\n lasmodium<\/jats:italic>\n male gamete differs from that of most other eukaryotes in that it is intracytoplasmic but retains a key conserved feature: axonemes assemble from basal bodies. The centriole\/basal body protein\n SAS<\/jats:styled-content>\n \u20106 normally regulates assembly and duplication of these organelles and its depletion causes severe flagellar\/ciliary abnormalities in a diverse array of eukaryotes. Since basal body and flagellum assembly are intimately coupled to male gamete development in\n \n P<\/jats:italic>\n <\/jats:styled-content>\n lasmodium<\/jats:italic>\n , we hypothesized that\n SAS<\/jats:styled-content>\n \u20106 disruption may cause gametogenesis defects and perturb transmission. We show that\n \n P<\/jats:italic>\n <\/jats:styled-content>\n lasmodium berghei<\/jats:italic>\n \u2005\n \n sas6<\/jats:italic>\n <\/jats:styled-content>\n knockouts display severely abnormal male gametogenesis presenting reduced basal body numbers, axonemal assembly defects and abnormal nuclear allocation. The defects in gametogenesis reduce fertilization and render\n \n Pbsas6<\/jats:italic>\n <\/jats:styled-content>\n knockouts less infectious to mosquitoes. Additionally, we show that lack of\n \n Pbsas6<\/jats:italic>\n <\/jats:styled-content>\n blocks transmission from mosquito to vertebrate host, revealing an additional yet undefined role in ookinete to sporulating oocysts transition. These findings underscore the vulnerability of the basal body\/\n SAS<\/jats:styled-content>\n \u20106 to malaria transmission blocking interventions.\n <\/jats:p>","DOI":"10.1111\/cmi.12355","type":"journal-article","created":{"date-parts":[[2014,8,26]],"date-time":"2014-08-26T00:29:03Z","timestamp":1409012943000},"page":"191-206","source":"Crossref","is-referenced-by-count":43,"title":["An essential role of the basal body protein\n SAS<\/scp>\n \u20106 in\n \n P<\/i>\n <\/scp>\n lasmodium<\/i>\n male gamete development and malaria transmission"],"prefix":"10.1155","volume":"17","author":[{"given":"Sara R.","family":"Marques","sequence":"first","affiliation":[{"name":"Department of Life Sciences Imperial College of London London SW7 2AZ UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chandra","family":"Ramakrishnan","sequence":"additional","affiliation":[{"name":"Institut f\u00fcr Parasitologie Universit\u00e4t Z\u00fcrich 8057 Z\u00fcrich 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