{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,22]],"date-time":"2025-10-22T17:54:18Z","timestamp":1761155658210},"reference-count":35,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2013,3,14]],"date-time":"2013-03-14T00:00:00Z","timestamp":1363219200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["CNS Neurosci Ther"],"published-print":{"date-parts":[[2013,5]]},"abstract":"<jats:title>Summary<\/jats:title><jats:sec><jats:title>Aims<\/jats:title><jats:p>In acute stroke, neurological damage is due to oxidative stress and neuronal apoptotic death. This study investigated whether Nogo\u2010A 290\u2010562 residues region (M9), fused to the transduction domain of the <jats:styled-content style=\"fixed-case\">HIV<\/jats:styled-content> trans\u2010activator (<jats:styled-content style=\"fixed-case\">TAT<\/jats:styled-content>) protein, is neuroprotective against cerebral ischemia and the mechanisms.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Transient focal cerebral ischemia was induced by middle cerebral artery occlusion in male C57<jats:styled-content style=\"fixed-case\">BL<\/jats:styled-content>\/6J mice. <jats:styled-content style=\"fixed-case\">TAT<\/jats:styled-content>\u2010M9, its mutation or vehicle was applied via intraperitoneal injection at the onset of reperfusion. The neurobehavioral scores, infarction volumes, neuronal apoptosis, and the ratio of Bax\/Bcl\u20102 were evaluated. Malondialdehyde (<jats:styled-content style=\"fixed-case\">MDA<\/jats:styled-content>), reactive oxygen species (<jats:styled-content style=\"fixed-case\">ROS<\/jats:styled-content>) levels, and <jats:styled-content style=\"fixed-case\">NADPH<\/jats:styled-content> oxidase activation were measured in the presence or absence of the <jats:styled-content style=\"fixed-case\">NADPH<\/jats:styled-content> oxidase inhibitor apocynin or activator tetrabromocinnamic acid (<jats:styled-content style=\"fixed-case\">TBCA<\/jats:styled-content>).<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Immunofluorescence results confirmed that <jats:styled-content style=\"fixed-case\">TAT<\/jats:styled-content>\u2010M9 was transduced into brain parenchyma, and it significantly improved neurological behavior, reduced infarct volumes, protected neuronal cells from apoptosis, inhibited activation of <jats:styled-content style=\"fixed-case\">NADPH<\/jats:styled-content> oxidase, and decreased <jats:styled-content style=\"fixed-case\">MDA<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">ROS<\/jats:styled-content> contents. Furthermore, apocynin imitated the beneficial effects of <jats:styled-content style=\"fixed-case\">TAT<\/jats:styled-content>\u2010M9, while <jats:styled-content style=\"fixed-case\">TBCA<\/jats:styled-content> abolished them.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Our results demonstrate that <jats:styled-content style=\"fixed-case\">TAT<\/jats:styled-content>\u2010M9 administration attenuates cerebral ischemia by inhibiting <jats:styled-content style=\"fixed-case\">NADPH<\/jats:styled-content> oxidase\u2010mediated oxidative damage and neuronal apoptosis in mice. <jats:styled-content style=\"fixed-case\">TAT<\/jats:styled-content>\u2010M9 may be a potential treatment for cerebrovascular disease.<\/jats:p><\/jats:sec>","DOI":"10.1111\/cns.12083","type":"journal-article","created":{"date-parts":[[2013,3,14]],"date-time":"2013-03-14T22:03:59Z","timestamp":1363298639000},"page":"319-328","source":"Crossref","is-referenced-by-count":11,"title":["M9, A Novel Region of Amino\u2010Nogo\u2010A, Attenuates Cerebral Ischemic Injury by Inhibiting <scp>NADPH<\/scp> Oxidase\u2010Derived Superoxide Production in Mice"],"prefix":"10.1111","volume":"19","author":[{"given":"Fan","family":"Guo","sequence":"first","affiliation":[{"name":"Department of Anesthesiology Xijing Hospital Fourth Military Medical University Xi'an China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wei\u2010Lin","family":"Jin","sequence":"additional","affiliation":[{"name":"Institute of Neurosciences School of Life Sciences and Biotechnology Shanghai Jiao Tong University Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Li\u2010Ya","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Anesthesiology Xijing Hospital Fourth Military Medical University Xi'an China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wen\u2010Ying","family":"Song","sequence":"additional","affiliation":[{"name":"Department of Anesthesiology Xijing Hospital Fourth Military Medical University Xi'an China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hui\u2010Wen","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Anesthesiology Xijing Hospital Fourth Military Medical University Xi'an China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xing\u2010Chun","family":"Gou","sequence":"additional","affiliation":[{"name":"Lab of Cell Biology &amp; 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