{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,2]],"date-time":"2026-01-02T17:27:51Z","timestamp":1767374871945},"reference-count":26,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2013,11,27]],"date-time":"2013-11-27T00:00:00Z","timestamp":1385510400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["CNS Neurosci Ther"],"published-print":{"date-parts":[[2014,2]]},"abstract":"<jats:title>Summary<\/jats:title><jats:sec><jats:title>Aims<\/jats:title><jats:p>To investigate whether Meserine, a novel phenylcarbamate derivative of (\u2212)\u2010meptazinol, possesses beneficial activities against cholinergic deficiency and amyloidogenesis, the two major pathological characteristics of Alzheimer's disease (<jats:styled-content style=\"fixed-case\">AD<\/jats:styled-content>).<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Ellman's assay and Morris water maze were used to detect acetylcholinesterase (AChE) activity and evaluate spatial learning and memory ability, respectively. Both high content screening and Western blotting were carried out to detect \u03b2\u2010amyloid precursor protein (APP), while RT\u2010PCR and ELISA were conducted to detect APP\u2010<jats:styled-content style=\"fixed-case\">mRNA<\/jats:styled-content> and \u03b2\u2010amyloid peptide (A\u03b2).<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>In scopolamine\u2010induced dementia mice, Meserine (1\u00a0mg\/kg, i.p.) significantly ameliorated spatial learning and memory deficits, which was consistent with its <jats:italic>in vitro<\/jats:italic> inhibitory ability against AChE (recombinant human AChE, IC<jats:sub>50<\/jats:sub>\u00a0=\u00a0274\u00a0\u00b1\u00a049 <jats:styled-content style=\"fixed-case\">nM<\/jats:styled-content>). Furthermore, Meserine (7.5 mg\/kg) injected intraperitoneally once daily for 3\u00a0weeks lowered APP level by 28% and A\u03b2<jats:sub>42<\/jats:sub> level by 42% in APP\/PS1 transgenic mouse cerebrum. This APP modulation action might be posttranscriptional, as Meserine reduced APP by about 30% in SH\u2010SY5Y\u2010APP<jats:sub>695<\/jats:sub> cells but did not alter APP\u2010<jats:styled-content style=\"fixed-case\">mRNA<\/jats:styled-content> level. And both APP and A\u03b2<jats:sub>42<\/jats:sub> lowering action of Meserine maintained longer than that of rivastigmine.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Meserine executes dual actions against cholinergic deficiency and amyloidogenesis and provides a promising lead compound for symptomatic and modifying therapy of <jats:styled-content style=\"fixed-case\">AD<\/jats:styled-content>.<\/jats:p><\/jats:sec>","DOI":"10.1111\/cns.12183","type":"journal-article","created":{"date-parts":[[2013,11,27]],"date-time":"2013-11-27T12:18:58Z","timestamp":1385554738000},"page":"165-171","source":"Crossref","is-referenced-by-count":15,"title":["Meserine, a Novel Carbamate <scp>AC<\/scp>hE Inhibitor, Ameliorates Scopolamine\u2010Induced Dementia and Alleviates Amyloidogenesis of <scp>APP<\/scp>\/<scp>PS<\/scp>1 Transgenic Mice"],"prefix":"10.1111","volume":"20","author":[{"given":"Bi\u2010Yun","family":"Shao","sequence":"first","affiliation":[{"name":"Department of Pharmacology &amp; Chemical Biology Institute of Medical Sciences Shanghai Jiao Tong University School of Medicine Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zheng","family":"Xia","sequence":"additional","affiliation":[{"name":"Department of Pharmacology &amp; Chemical Biology Institute of Medical Sciences Shanghai Jiao Tong University School of Medicine Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qiong","family":"Xie","sequence":"additional","affiliation":[{"name":"Department of Medicinal Chemistry School of Pharmacy Fudan University Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xin\u2010Xing","family":"Ge","sequence":"additional","affiliation":[{"name":"Department of Pharmacology &amp; Chemical Biology Institute of Medical Sciences Shanghai Jiao Tong University School of Medicine Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wei\u2010Wei","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Pharmacology &amp; 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