{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,25]],"date-time":"2025-10-25T12:16:06Z","timestamp":1761394566486},"reference-count":59,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2014,12,1]],"date-time":"2014-12-01T00:00:00Z","timestamp":1417392000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"The Rasmussen's Encephalitis Children\u00e2\u20ac\u2122s Project."}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["CNS Neurosci Ther"],"published-print":{"date-parts":[[2015,2]]},"abstract":"<jats:title>Summary<\/jats:title><jats:sec><jats:title>Aims<\/jats:title><jats:p>Rasmussen encephalitis (<jats:styled-content style=\"fixed-case\">RE<\/jats:styled-content>) is a rare but devastating condition, mainly in children, characterized by sustained brain inflammation, atrophy of one cerebral hemisphere, epilepsy, and progressive cognitive deterioration. The etiology of <jats:styled-content style=\"fixed-case\">RE<\/jats:styled-content>\u2010induced seizures associated with the inflammatory process remains unknown.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Cortical tissue samples from children undergoing surgical resections for the treatment of <jats:styled-content style=\"fixed-case\">RE<\/jats:styled-content> (n\u00a0=\u00a016) and non\u2010<jats:styled-content style=\"fixed-case\">RE<\/jats:styled-content> (n\u00a0=\u00a012) were compared using electrophysiological, morphological, and immunohistochemical techniques to examine neuronal properties and the relationship with microglial activation using the specific microglia\/macrophage calcium\u2010binding protein, <jats:styled-content style=\"fixed-case\">IBA<\/jats:styled-content>1 in conjunction with connexins and pannexin expression.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Compared with non\u2010<jats:styled-content style=\"fixed-case\">RE<\/jats:styled-content> cases, pyramidal neurons from <jats:styled-content style=\"fixed-case\">RE<\/jats:styled-content> cases displayed increased cell capacitance and reduced input resistance. However, neuronal somatic areas were not increased in size. Instead, intracellular injection of biocytin led to increased dye coupling between neurons from <jats:styled-content style=\"fixed-case\">RE<\/jats:styled-content> cases. By Western blot, expression of <jats:styled-content style=\"fixed-case\">IBA<\/jats:styled-content>1 and pannexin was increased while connexin 32 was decreased in <jats:styled-content style=\"fixed-case\">RE<\/jats:styled-content> cases compared with non\u2010<jats:styled-content style=\"fixed-case\">RE<\/jats:styled-content> cases. <jats:styled-content style=\"fixed-case\">IBA<\/jats:styled-content>1 immunostaining overlapped with pannexin and connexin 36 in <jats:styled-content style=\"fixed-case\">RE<\/jats:styled-content> cases.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>In <jats:styled-content style=\"fixed-case\">RE<\/jats:styled-content>, these results support the notion that a possible mechanism for cellular hyperexcitability may be related to increased intercellular coupling from pannexin linked to increased microglial activation. Such findings suggest that a possible antiseizure treatment for <jats:styled-content style=\"fixed-case\">RE<\/jats:styled-content> may involve the use of gap junction blockers.<\/jats:p><\/jats:sec>","DOI":"10.1111\/cns.12352","type":"journal-article","created":{"date-parts":[[2014,12,1]],"date-time":"2014-12-01T10:40:51Z","timestamp":1417430451000},"page":"152-163","source":"Crossref","is-referenced-by-count":30,"title":["In Rasmussen Encephalitis, Hemichannels Associated with Microglial Activation are linked to Cortical Pyramidal Neuron Coupling: A Possible Mechanism for Cellular Hyperexcitability"],"prefix":"10.1111","volume":"21","author":[{"given":"Carlos","family":"Cepeda","sequence":"first","affiliation":[{"name":"Intellectual and Developmental Disabilities Research Center Brain Research Institute Los Angeles CA USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Julia W.","family":"Chang","sequence":"additional","affiliation":[{"name":"Department of Neurosurgery and Psychiatry &amp; BioBehavioral Medicine Mattel Children's Hospital David Geffen School of Medicine University of California Los Angeles Los Angeles CA USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Geoffrey C.","family":"Owens","sequence":"additional","affiliation":[{"name":"Department of Neurosurgery and Psychiatry &amp; BioBehavioral Medicine Mattel Children's Hospital David Geffen School of Medicine University of California Los Angeles Los Angeles CA USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"My N.","family":"Huynh","sequence":"additional","affiliation":[{"name":"Department of Neurosurgery and Psychiatry &amp; BioBehavioral Medicine Mattel Children's Hospital David Geffen School of Medicine University of California Los Angeles Los Angeles CA USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jane Y.","family":"Chen","sequence":"additional","affiliation":[{"name":"Intellectual and Developmental Disabilities Research Center Brain Research Institute Los Angeles CA USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Conny","family":"Tran","sequence":"additional","affiliation":[{"name":"Intellectual and Developmental Disabilities Research Center Brain Research Institute Los Angeles CA USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Harry V.","family":"Vinters","sequence":"additional","affiliation":[{"name":"Section of Neuropathology Department of Neurology Mattel Children's Hospital David Geffen School of Medicine University of California Los Angeles Los Angeles CA USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael S.","family":"Levine","sequence":"additional","affiliation":[{"name":"Intellectual and Developmental Disabilities Research Center Brain Research Institute Los Angeles CA USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gary 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