{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,11]],"date-time":"2026-10-11T13:53:07Z","timestamp":1791726787448,"version":"4.3.4"},"reference-count":29,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2015,2,10]],"date-time":"2015-02-10T00:00:00Z","timestamp":1423526400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81160168"],"award-info":[{"award-number":["81160168"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["CNS Neurosci Ther"],"published-print":{"date-parts":[[2015,6]]},"abstract":"<jats:title>Summary<\/jats:title><jats:sec><jats:title>Aims<\/jats:title><jats:p>Studies showed fastigial nucleus stimulation (<jats:styled-content style=\"fixed-case\">FNS<\/jats:styled-content>) reduced brain damage, but the mechanisms of neuroprotection induced by <jats:styled-content style=\"fixed-case\">FNS<\/jats:styled-content> were not entirely understood; Micro<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>s are noncoding <jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> molecules that regulate gene expression in a posttranscriptional manner, but their functional consequence in response to ischemia\u2013reperfusion (<jats:styled-content style=\"fixed-case\">IR<\/jats:styled-content>) remains unknown. We investigated the role of micro<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>\u201029c in the neuroprotection induced by <jats:styled-content style=\"fixed-case\">FNS<\/jats:styled-content> in rat.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>The <jats:styled-content style=\"fixed-case\">IR<\/jats:styled-content> rat models were conducted 1\u00a0day after <jats:styled-content style=\"fixed-case\">FNS<\/jats:styled-content>. Besides, miR\u201029c antagomir (or agomir or control) was infused to the left intracerebroventricular 1\u00a0day before <jats:styled-content style=\"fixed-case\">IR<\/jats:styled-content> models were conducted. We detected differential expression of Birc2 <jats:styled-content style=\"fixed-case\">mRNA<\/jats:styled-content> (also Bak1m<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> and miR\u201029c) level among different groups by <jats:styled-content style=\"fixed-case\">RT<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">qPCR<\/jats:styled-content>. The differential expression of Birc2 protein (also Bak1 protein) level among different groups was surveyed via Western blot. The neuroprotective effects were assessed by infarct volume, neurological deficit, and apoptosis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>MiR\u201029c was decreased after <jats:styled-content style=\"fixed-case\">FNS<\/jats:styled-content>. Moreover, miR\u201029c directly bound to the predicted 3\u2032\u2010<jats:styled-content style=\"fixed-case\">UTR<\/jats:styled-content> target sites of Birc2\u00a0and Bak1 genes. Furthermore, over\u2010expression of miR\u201029c effectively reduced Birc2 (also Bak1) <jats:styled-content style=\"fixed-case\">mRNA<\/jats:styled-content> and protein levels, increased infarct volume and apoptosis, and deteriorated neurological outcomes, whereas down\u2010regulation played a neuroprotective role.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>MiR\u201029c correlates with the neuroprotection induced by <jats:styled-content style=\"fixed-case\">FNS<\/jats:styled-content> by negatively regulating Birc2 and Bak1.<\/jats:p><\/jats:sec>","DOI":"10.1111\/cns.12383","type":"journal-article","created":{"date-parts":[[2015,2,12]],"date-time":"2015-02-12T21:51:01Z","timestamp":1423777861000},"page":"496-503","source":"Crossref","is-referenced-by-count":38,"title":["Micro<scp>RNA<\/scp>\u201029c Correlates with Neuroprotection Induced by <scp>FNS<\/scp> by Targeting Both Birc2 and Bak1 in Rat Brain after Stroke"],"prefix":"10.1111","volume":"21","author":[{"given":"Li\u2010Gang","family":"Huang","sequence":"first","affiliation":[{"name":"Department of Neurology The First Affiliated Hospital Guangxi Medical University Nanning China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jin\u2010Pin","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Neurology The First Affiliated Hospital Guangxi Medical University Nanning China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiao\u2010Min","family":"Pang","sequence":"additional","affiliation":[{"name":"Department of Neurology The First Affiliated Hospital Guangxi Medical University Nanning China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chun\u2010Yong","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Neurology The First Affiliated Hospital Guangxi Medical University Nanning 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