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Recent reports show that 7,8\u2010dihydroxyflavone (\n DHF<\/jats:styled-content>\n ), a TrkB agonist, has beneficial effects in animal model of\n PD<\/jats:styled-content>\n . However, it is unclear whether the therapeutic effects of\n DHF<\/jats:styled-content>\n are associated with the expression of\n \u03b1<\/jats:italic>\n \u2010synuclein.\n <\/jats:p>\n <\/jats:sec>\n \n Aims<\/jats:title>\n \n In this study, we investigated the protective effects of\n DHF<\/jats:styled-content>\n on 1\u2010methyl\u20104\u2010phenyl\u20101,2,3,6\u2010tetrahydropyridine (\n MPTP<\/jats:styled-content>\n )\u2010induced deficit of motor functions, the loss of dopaminergic neurons and the expression of\n \u03b1<\/jats:italic>\n \u2010synuclein as well as antioxidative activity in the C57\n BL<\/jats:styled-content>\n \/6 mice.\n <\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n \n Mice were treated with\n MPTP<\/jats:styled-content>\n (30 mg\/kg, i.p.) once a day for 5 days to induce dopaminergic neuron death in the\n SN<\/jats:styled-content>\n .\n DHF<\/jats:styled-content>\n (5 mg\/kg, i.p.) was administrated once a day from the first day of\n MPTP<\/jats:styled-content>\n injection until 9 days after the last injection of\n MPTP<\/jats:styled-content>\n . Behavioral tests showed that\n DHF<\/jats:styled-content>\n succeeded in ameliorating the impaired motor functions in the\n MPTP<\/jats:styled-content>\n \u2010treated mice. The immunohistochemical assay showed that the amelioration of motor function was accompanied by a reduction in the loss of dopaminergic neurons in the\n SN<\/jats:styled-content>\n and striatum. Western blot analyses showed that\n DHF<\/jats:styled-content>\n prevented the inactivation of TrkB and suppressed\n \u03b1<\/jats:italic>\n \u2010synuclein overexpression in the\n SN<\/jats:styled-content>\n and striatum following\n MPTP<\/jats:styled-content>\n treatment. Antioxidative activity detection revealed that\n DHF<\/jats:styled-content>\n prevented\n MPTP<\/jats:styled-content>\n \u2010induced reduction in glutathione and total superoxide dismutase activity in the\n SN<\/jats:styled-content>\n and striatum.\n <\/jats:p>\n <\/jats:sec>\n \n Conclusion<\/jats:title>\n \n Taken together, these results indicate that\n DHF<\/jats:styled-content>\n treatment may suppress the accumulation of\n \u03b1<\/jats:italic>\n \u2010synuclein and oxidative stress via activating TrkB and subsequently block the loss of dopaminergic neurons in the\n SN<\/jats:styled-content>\n and striatum, thereby ameliorating\n MPTP<\/jats:styled-content>\n \u2010induced motor deficits in the C57\n BL<\/jats:styled-content>\n \/6 mice.\n <\/jats:p>\n <\/jats:sec>","DOI":"10.1111\/cns.12555","type":"journal-article","created":{"date-parts":[[2016,4,15]],"date-time":"2016-04-15T07:28:50Z","timestamp":1460705330000},"page":"617-624","source":"Crossref","is-referenced-by-count":64,"title":["7,8\u2010dihydroxyflavone Ameliorates Motor Deficits Via Suppressing\n \u03b1<\/i>\n \u2010synuclein Expression and Oxidative Stress in the\n MPTP<\/scp>\n \u2010induced Mouse Model of Parkinson's Disease"],"prefix":"10.1111","volume":"22","author":[{"given":"Xiao\u2010Huan","family":"Li","sequence":"first","affiliation":[{"name":"Chongqing Key Laboratory of Translational Medical Research in Cognitive Development and Learning and Memory Disorders Children's Hospital of Chongqing Medical University Chongqing China"},{"name":"Ministry of Education Key Laboratory of Child Development and Disorders Children's Hospital of Chongqing Medical University Chongqing 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