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AMD3100, a highly selective CXC\u2010chemokine receptor 4 (CXCR4) antagonist, was used to inhibit microglia migration. Microglia was evaluated by immunofluorescence <jats:italic>in\u00a0vivo<\/jats:italic>, and their migration was tested by transwell assay <jats:italic>in\u00a0vitro<\/jats:italic>. Expressions of cytokines were detected by real\u2010time PCR. Infarct volume was determined by triphenyltetrazolium chloride (TTC) staining. Functional recovery of tMCAO rats was evaluated by behavior tests.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>M1 microglia in the IBZ was rapidly increased within 3\u00a0days after tMCAO, accompanied with enhanced expression of CXCR4. Chemokine CXC motif chemokine ligand 12 (CXCL12) was also increased in the IBZ. And AMD3100 could obviously decline M1 microglia migration induced by CXCL12 and secretion of related inflammatory cytokines in the IBZ after stroke. This was accompanied by significant attenuated infarct volume and improved neurological outcomes.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>This study confirms the protective efficacy of inhibiting microglia migration at the hyperacute phase as a therapeutic strategy for ischemic stroke in tMCAO model of rats, and its therapeutic time window could last for 24\u00a0h after cerebral ischemia reperfusion.<\/jats:p><\/jats:sec>","DOI":"10.1111\/cns.12665","type":"journal-article","created":{"date-parts":[[2016,12,19]],"date-time":"2016-12-19T10:12:50Z","timestamp":1482142370000},"page":"222-232","source":"Crossref","is-referenced-by-count":34,"title":["Inhibiting the Migration of M1 Microglia at Hyperacute Period Could Improve Outcome of tMCAO Rats"],"prefix":"10.1111","volume":"23","author":[{"given":"Ming","family":"Huang","sequence":"first","affiliation":[{"name":"Department of Neurology Union Hospital Tongji Medical College Huazhong University of 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