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Annexin A2 (AnxA2) is an important signaling protein involved in several intracellular processes such as membrane trafficking, endocytosis, and exocytosis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Aim<\/jats:title><jats:p>To investigate the roles and mechanism of AnxA2 during cryptococcal transversal of <jats:styled-content style=\"fixed-case\">BMEC<\/jats:styled-content>s.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p><jats:italic>Cryptococcus neoformans<\/jats:italic> infection initiated upregulation of AnxA2 in mouse <jats:styled-content style=\"fixed-case\">BMEC<\/jats:styled-content>s. Blockade with anti\u2010AnxA2 antibody led to a reduction in fungal transcytosis activity but no change in its adhesion efficiency. Intriguingly, AnxA2 depletion caused a significant increase in fungal association activity but had no effect on their transcytosis. AnxA2 suppression resulted in marked reduction in its partner protein S100A10, and S100A10 suppression in <jats:styled-content style=\"fixed-case\">BMEC<\/jats:styled-content>s significantly reduced the cryptococcal transcytosis efficiency. Furthermore, AnxA2 dephosphorylation at Tyr23 and dephosphorylation of downstream cofilin were required for cryptococcal transversal of <jats:styled-content style=\"fixed-case\">BMEC<\/jats:styled-content>s, both of which might be primarily involved in the association of <jats:italic>C. neoformans<\/jats:italic> with host cells.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Our work indicated that AnxA2 played complex roles in traversal of <jats:italic>C. neoformans<\/jats:italic> across host <jats:styled-content style=\"fixed-case\">BMEC<\/jats:styled-content>s, which might be dependent on downstream cofilin to inhibit fungal adhesion but rely on its partner S100A10 to promote cryptococcal transcytosis.<\/jats:p><\/jats:sec>","DOI":"10.1111\/cns.12673","type":"journal-article","created":{"date-parts":[[2017,1,28]],"date-time":"2017-01-28T05:29:19Z","timestamp":1485581359000},"page":"291-300","source":"Crossref","is-referenced-by-count":24,"title":["Complex Roles of Annexin A2 in Host Blood\u2013Brain Barrier Invasion by <i>Cryptococcus neoformans<\/i>"],"prefix":"10.1111","volume":"23","author":[{"given":"Wei","family":"Fang","sequence":"first","affiliation":[{"name":"PLA Key Laboratory of Mycosis Department of Dermatology and Venereology Changzheng Hospital Shanghai China"},{"name":"Shanghai Key Laboratory of Molecular Medical Mycology Shanghai Institute of Medical Mycology Second Military Medical University Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhen\u2010Zong","family":"Fa","sequence":"additional","affiliation":[{"name":"Shanghai Key Laboratory of Molecular Medical Mycology Shanghai Institute of Medical Mycology Second Military 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