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The <jats:italic>zif\u2010268 <\/jats:italic><jats:styled-content style=\"fixed-case\">mRNA<\/jats:styled-content> expression was additionally analyzed by in situ hybridization in several brain structures.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>5\u2032Cl5\u2032d\u2010(\u00b1)\u2010<jats:styled-content style=\"fixed-case\">ENBA<\/jats:styled-content> (0.05\u20100.5\u00a0mg\/kg ip) dose dependently reduced the harmaline\u2010induced tremor and this effect was reversed by 8\u2010cyclopentyl\u20101,3\u2010dipropylxanthine (<jats:styled-content style=\"fixed-case\">DPCPX<\/jats:styled-content>), a selective antagonist of adenosine A<jats:sub>1<\/jats:sub> receptors (1\u00a0mg\/kg ip). Harmaline increased the <jats:italic>zif\u2010268 <\/jats:italic><jats:styled-content style=\"fixed-case\">mRNA<\/jats:styled-content> expression in the inferior olive, cerebellar cortex, ventroanterior\/ventrolateral thalamic nuclei, and motor cortex. 5\u2032Cl5\u2032d\u2010(\u00b1)\u2010<jats:styled-content style=\"fixed-case\">ENBA<\/jats:styled-content> reversed these increases in all the above structures. <jats:styled-content style=\"fixed-case\">DPCPX<\/jats:styled-content> reduced the effect of 5\u2032Cl5\u2032d\u2010(\u00b1)\u2010<jats:styled-content style=\"fixed-case\">ENBA<\/jats:styled-content> on <jats:italic>zif\u2010268 <\/jats:italic><jats:styled-content style=\"fixed-case\">mRNA<\/jats:styled-content> in the motor cortex.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>This study suggests that adenosine A<jats:sub>1<\/jats:sub> receptors may be a potential target for the treatment of essential tremor.<\/jats:p><\/jats:sec>","DOI":"10.1111\/cns.12692","type":"journal-article","created":{"date-parts":[[2017,3,30]],"date-time":"2017-03-30T04:43:25Z","timestamp":1490849005000},"page":"438-446","source":"Crossref","is-referenced-by-count":14,"title":["Tremorolytic effect of 5\u2032\u2010chloro\u20105\u2032\u2010deoxy\u2010(\u00b1)\u2010<scp>ENBA<\/scp>, a potent and selective adenosine A1 receptor agonist, evaluated in the harmaline\u2010induced model in rats"],"prefix":"10.1111","volume":"23","author":[{"given":"Barbara","family":"Kosmowska","sequence":"first","affiliation":[{"name":"Department of Neuro\u2010Psychopharmacology Institute of Pharmacology Polish Academy of Sciences  Krak\u00f3w Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8381-6632","authenticated-orcid":false,"given":"Krystyna","family":"Ossowska","sequence":"additional","affiliation":[{"name":"Department of Neuro\u2010Psychopharmacology Institute of 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