{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,14]],"date-time":"2026-03-14T21:53:39Z","timestamp":1773525219806,"version":"3.50.1"},"reference-count":56,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2018,3,25]],"date-time":"2018-03-25T00:00:00Z","timestamp":1521936000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100003819","name":"Natural Science Foundation of Hubei Province","doi-asserted-by":"publisher","award":["2017CFA017"],"award-info":[{"award-number":["2017CFA017"]}],"id":[{"id":"10.13039\/501100003819","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["CNS Neurosci Ther"],"published-print":{"date-parts":[[2018,10]]},"abstract":"<jats:title>Summary<\/jats:title><jats:sec><jats:title>Aim<\/jats:title><jats:p>Multiple sclerosis (<jats:styled-content style=\"fixed-case\">MS<\/jats:styled-content>) is a neurological autoimmune disorder characterized by mistaken attacks of inflammatory cells against the central nervous system (<jats:styled-content style=\"fixed-case\">CNS<\/jats:styled-content>), resulting in demyelination and axonal damage. Kv1.3 channel blockers can inhibit T\u2010cell activation and have been designed for <jats:styled-content style=\"fixed-case\">MS<\/jats:styled-content> therapy. However, little is known about the effects of Kv1.3 blockers on protecting myelin sheaths\/axons in <jats:styled-content style=\"fixed-case\">MS<\/jats:styled-content>. This study aimed at investigating the neuroprotection efficacy of a selective Kv1.3 channel blocker Im<jats:styled-content style=\"fixed-case\">KT<\/jats:styled-content>x88 (ImK) in <jats:styled-content style=\"fixed-case\">MS<\/jats:styled-content> animal model.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Experimental autoimmune encephalomyelitis (<jats:styled-content style=\"fixed-case\">EAE<\/jats:styled-content>) rat model was established. The neuroprotective effect of ImK was assessed by immunohistochemistry and transmission electron microscopy (<jats:styled-content style=\"fixed-case\">TEM<\/jats:styled-content>). In addition, the antiinflammatory effect of ImK by suppressing T\u2010cell activation was assessed by flow cytometry and <jats:styled-content style=\"fixed-case\">ELISA<\/jats:styled-content> in vitro.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Our results demonstrated that ImK administration ameliorated <jats:styled-content style=\"fixed-case\">EAE<\/jats:styled-content> clinical severity. Moreover, ImK increased oligodendrocytes survival, preserved axons, and myelin integrity and reduced the infiltration of activated T cells into the <jats:styled-content style=\"fixed-case\">CNS<\/jats:styled-content>. This protective effect of the peptide may be related to its suppression of autoantigen\u2010specific T\u2010cell activation via calcium influx inhibition.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>ImK prevents neurological damage by suppressing T\u2010cell activation, suggesting the applicability of this peptide in <jats:styled-content style=\"fixed-case\">MS<\/jats:styled-content> therapy.<\/jats:p><\/jats:sec>","DOI":"10.1111\/cns.12848","type":"journal-article","created":{"date-parts":[[2018,3,26]],"date-time":"2018-03-26T01:06:33Z","timestamp":1522026393000},"page":"967-977","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":21,"title":["A Kv1.3 channel\u2010specific blocker alleviates neurological impairment through inhibiting T\u2010cell activation in experimental autoimmune 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