{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,12]],"date-time":"2026-05-12T06:47:37Z","timestamp":1778568457500,"version":"3.51.4"},"reference-count":33,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2019,5,13]],"date-time":"2019-05-13T00:00:00Z","timestamp":1557705600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100008871","name":"Yunnan Provincial Science and Technology Department","doi-asserted-by":"publisher","award":["2013C227"],"award-info":[{"award-number":["2013C227"]}],"id":[{"id":"10.13039\/501100008871","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["CNS Neurosci Ther"],"published-print":{"date-parts":[[2020,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Neural stem cells (NSCs) transplantation is considered a promising treatment for Parkinson's disease. But most NSCs are differentiated into glial cells rather than neurons, and only a few of them survive after transplantation due to the inflammatory environment.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>In this study, neural stem cells (NSCs) and microglial cells both forced with the <jats:italic>Nurr1<\/jats:italic> gene were transplanted into the striatum of the rat model of PD. The results were evaluated through reverse transcription polymerase chain reaction (RT\u2010PCR), Western blot, and immunofluorescence analysis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The behavioral abnormalities of PD rats were improved by combined transplantation of NSCs and microglia, both forced with <jats:italic>Nurr1<\/jats:italic>. The number of tyrosine hydroxylase+ cells in the striatum of PD rats increased, and the number of Iba1+ cells decreased compared with the other groups. Moreover, the dopamine neurons differentiated from grafted NSCs could still be detected in the striatum of PD rats after 5\u00a0months.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>The results suggested that transplantation of Nurr1\u2010overexpressing NSCs and microglia could improve the inhospitable host brain environments, which will be\u00a0 a new potential strategy for the cell replacement therapy in PD.<\/jats:p><\/jats:sec>","DOI":"10.1111\/cns.13149","type":"journal-article","created":{"date-parts":[[2019,5,14]],"date-time":"2019-05-14T04:14:46Z","timestamp":1557807286000},"page":"55-65","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":21,"title":["Transplantation of Nurr1\u2010overexpressing neural stem cells and microglia for treating parkinsonian rats"],"prefix":"10.1111","volume":"26","author":[{"given":"Yuan","family":"Qian","sequence":"first","affiliation":[{"name":"Yunnan Key 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