{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,20]],"date-time":"2026-07-20T05:59:09Z","timestamp":1784527149746,"version":"3.55.0"},"reference-count":45,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2021,8,4]],"date-time":"2021-08-04T00:00:00Z","timestamp":1628035200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["CNS Neurosci Ther"],"published-print":{"date-parts":[[2021,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Parkinson's disease (PD) is a neurodegenerative disease, and its pathogenesis is unclear. Previous studies mainly focus on the lesions of substantia nigra (SN) and striatum (Str) in PD. However, lesions are not limited. The olfactory bulb (OB), subventricular zone (SVZ), and hippocampus (Hippo) are also affected in PD.<\/jats:p><\/jats:sec><jats:sec><jats:title>Aim<\/jats:title><jats:p>To reveal gene expression changes in the five brain regions (OB, SVZ, Str, SN, and Hippo), and to look for potential candidate genes and pathways that may be correlated with the pathogenesis of PD.<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and methods<\/jats:title><jats:p>We established control group and 6\u2010hydroxydopamine (6\u2010OHDA) PD model group, and detected gene expressions in the five brain regions using RNA\u2010seq and real\u2010time quantitative polymerase chain reaction (RT\u2010qPCR). We further analyzed the RNA\u2010seq data by bioinformatics.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>We identified differentially expressed genes (DEGs) in all five brain regions. The DEGs were significantly enriched in the \u201cdopaminergic synapse\u201d and \u201cretrograde endocannabinoid signaling,\u201d and Gi\/o\u2010GIRK is the shared cascade in the two pathways. We further identified Ephx2, Fam111a, and Gng2 as the potential candidate genes in the pathogenesis of PD for further studies.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Our study suggested that gene expressions change in the five brain regions following exposure to 6\u2010OHDA. The \u201cdopaminergic synapse,\u201d \u201cretrograde endocannabinoid signaling,\u201d and Gi\/o\u2010GIRK may be the key pathways and cascade of the synaptic damage in 6\u2010OHDA PD rats. Ephx2, Fam111a, and Gng2 may play critical roles in the pathogenesis of PD.<\/jats:p><\/jats:sec>","DOI":"10.1111\/cns.13702","type":"journal-article","created":{"date-parts":[[2021,8,4]],"date-time":"2021-08-04T12:55:20Z","timestamp":1628081720000},"page":"1289-1299","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Transcriptome profiling of five brain regions in a 6\u2010hydroxydopamine rat model of Parkinson\u2019s disease"],"prefix":"10.1111","volume":"27","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1598-6581","authenticated-orcid":false,"given":"Ying","family":"Lyu","sequence":"first","affiliation":[{"name":"Institute of Laboratory Animal Sciences (ILAS), Chinese Academy of Medical Sciences (CAMS) &amp; Comparative Medical Center Peking Union Medical College (PUMC) Beijing China"},{"name":"Beijing Engineering Research Center for Experimental Animal Models of Human Critical Diseases Beijing China"},{"name":"Guangzhou Medical University Guangzhou Guangdong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yiying","family":"Huang","sequence":"additional","affiliation":[{"name":"Institute of Laboratory Animal Sciences (ILAS), Chinese Academy of Medical Sciences (CAMS) &amp; Comparative Medical Center Peking Union Medical College (PUMC) Beijing China"},{"name":"Beijing Engineering Research Center for Experimental Animal Models of Human Critical Diseases Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guiying","family":"Shi","sequence":"additional","affiliation":[{"name":"Institute of Laboratory Animal Sciences (ILAS), Chinese Academy of Medical Sciences (CAMS) &amp; Comparative Medical Center Peking Union Medical College (PUMC) Beijing China"},{"name":"Beijing Engineering Research Center for Experimental Animal Models of Human Critical Diseases Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xuepei","family":"Lei","sequence":"additional","affiliation":[{"name":"Institute of Laboratory Animal Sciences (ILAS), Chinese Academy of Medical Sciences (CAMS) &amp; Comparative Medical Center Peking Union Medical College (PUMC) Beijing China"},{"name":"Beijing Engineering Research Center for Experimental Animal Models of Human Critical Diseases Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Keya","family":"Li","sequence":"additional","affiliation":[{"name":"Institute of Laboratory Animal Sciences (ILAS), Chinese Academy of Medical Sciences (CAMS) &amp; 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