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The majority of HE results from hypertensive ICH. Previous study reported higher iron content in the brains of hypertensive patients. Iron overload exacerbates the risk of hemorrhagic transformation in thromboembolic stroke mice. Whether iron overload during the process of hypertension participates in secondary bleeding of hypertensive ICH remains unclear.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Hypertension was induced by continuous infusion of angiotensin II (Ang II) with an osmotic pump into C57BL\/6 mice. ICH was simulated by intrastriatal injection of the liquid polymer Onyx\u201018. Iron chelation and iron overload was achieved by deferoxamine mesylate or iron dextran injection. Secondary bleeding was quantified by measuring the hemoglobin content in the ipsilateral brain hemisphere.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Ang II\u2010induced hypertensive mice showed increased iron accumulation in the brain and expanded secondary hemorrhage after ICH modeling. Moreover, iron chelation suppressed while iron overload aggravated secondary bleeding. Mechanistically, iron exacerbated the loss of contractile cerebral vascular smooth muscle cells (VSMCs), aggravated blood\u2013brain barrier (BBB) leakage in Ang II\u2010induced hypertensive mice, and increased glial and MMP9 accumulation after ICH.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Iron overload plays a key role in secondary bleeding after ICH in Ang II\u2010induced hypertensive mice. Iron chelation during the process of Ang II\u2010induced hypertension suppresses secondary bleeding after ICH.<\/jats:p><\/jats:sec>","DOI":"10.1111\/cns.13706","type":"journal-article","created":{"date-parts":[[2021,8,4]],"date-time":"2021-08-04T11:51:16Z","timestamp":1628077876000},"page":"1327-1338","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Iron chelation suppresses secondary bleeding after intracerebral hemorrhage in angiotensin II\u2010infused mice"],"prefix":"10.1111","volume":"27","author":[{"given":"Jie","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Neurosurgery Southwest Hospital Third Military Medical University (Army Medical University Chongqing China"},{"name":"State Key Laboratory of Trauma, Burn and Combined Injury Third Military Medical University Chongqing China"},{"name":"Chongqing Key Laboratory of Precision Neuromedicine and Neuroregenaration Southwest Hospital 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