{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,19]],"date-time":"2026-02-19T19:11:31Z","timestamp":1771528291030,"version":"3.50.1"},"reference-count":58,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2021,9,30]],"date-time":"2021-09-30T00:00:00Z","timestamp":1632960000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81671166"],"award-info":[{"award-number":["81671166"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["CNS Neurosci Ther"],"published-print":{"date-parts":[[2022,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objective<\/jats:title><jats:p>Hemorrhagic transformation (HT) is a life\u2010threatening complication of stroke. Whether changes in gut microbial composition underlie the development of HT remains unknown. This study aimed to investigate whether the gut microbiota is altered in HT rats and examine the association between these changes and inflammatory responses.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>HT was successfully established in rats injected with 50% glucose (6\u00a0ml\/Kg, i.p.) 15\u00a0min before middle cerebral artery occlusion (MCAO, 90\u00a0min occlusion) with reperfusion. After 5\u00a0days, rats were euthanized, and their brains used to estimate infarct volume. The inflammatory factors, the analysis of gut microbiota, and short\u2010chain fatty acids (SCFA) were assessed.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>In contrast with non\u2010HT rats, gut microbiota sequencing showed an elevation in the relative abundance of Proteobacteria and Actinobacteria in HT rats. Total SCFAs, especially butyrate and valeric acid, were significantly lower in the cecal contents of HT rats than in those of non\u2010HT rats. Hyperglycemia\u2010induced HT exacerbation was not observed when rats were treated with antibiotics, suggesting that altered microbiota play a critical role in hyperglycemic HT pathogenesis. Furthermore, rats whose gut was colonized with HT rat microbiota showed increased susceptibility to HT.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>This study provides important information about the gut microbiota profiles and SCFA levels of MCAO rats with HT or non\u2010HT. The susceptibility to HT in MCAO rats is associated with inflammation and gut microbiota modulation.<\/jats:p><\/jats:sec>","DOI":"10.1111\/cns.13736","type":"journal-article","created":{"date-parts":[[2021,9,30]],"date-time":"2021-09-30T19:24:57Z","timestamp":1633029897000},"page":"77-91","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":35,"title":["Alterations in the gut microbiome with hemorrhagic transformation in experimental stroke"],"prefix":"10.1111","volume":"28","author":[{"given":"Qin","family":"Huang","sequence":"first","affiliation":[{"name":"Department of neurology Xiangya Hospital Central South University  Changsha Hunan P.R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Liao","family":"Di","sequence":"additional","affiliation":[{"name":"Department of neurology Xiangya Hospital Central South University  Changsha Hunan P.R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fang","family":"Yu","sequence":"additional","affiliation":[{"name":"Department of neurology Xiangya Hospital Central South University  Changsha Hunan P.R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xianjing","family":"Feng","sequence":"additional","affiliation":[{"name":"Department of neurology Xiangya Hospital Central South University  Changsha Hunan P.R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zeyu","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of neurology Xiangya Hospital Central South University  Changsha Hunan P.R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Minping","family":"Wei","sequence":"additional","affiliation":[{"name":"Department of neurology Xiangya Hospital Central South University  Changsha Hunan P.R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yunfang","family":"Luo","sequence":"additional","affiliation":[{"name":"Department of neurology Xiangya Hospital Central South University  Changsha Hunan P.R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9154-131X","authenticated-orcid":false,"given":"Jian","family":"Xia","sequence":"additional","affiliation":[{"name":"Department of neurology Xiangya Hospital Central South University  Changsha Hunan P.R. China"},{"name":"Hunan Clinical Research Center for Cerebrovascular Disease  Changsha China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2021,9,30]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12975-015-0391-0"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/micr.20351"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1212\/WNL.41.6.899"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1161\/hs1201.99512"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.expneurol.2014.12.016"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.119.316448"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12975-020-00863-4"},{"key":"e_1_2_9_9_1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1155\/2015\/395895","article-title":"Neuroprotective Effect of Sodium Butyrate against Cerebral Ischemia\/Reperfusion Injury in Mice","volume":"2015","author":"Sun J","year":"2015","journal-title":"Biomed Res Int"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1126\/scitranslmed.aay6218"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pbio.3000410"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1161\/STROKEAHA.115.011258"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.STR.26.4.627"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1097\/01.WCB.0000073946.29308.55"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2377-7-33"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.lfs.2005.10.030"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.STR.28.11.2296"},{"issue":"1","key":"e_1_2_9_18_1","article-title":"Anti\u2010high mobility group box\u20101 (HMGB1) antibody inhibits hemorrhage\u2010induced brain injury and improved neurological deficits in rats","volume":"7","author":"Wang D","year":"2017","journal-title":"Sci Rep"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00442-004-1736-7"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkl244"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1000080107"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-019-48749-2"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41398-020-01038-3"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.brainres.2020.147056"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1161\/STROKEAHA.120.029262"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1002\/ana.25250"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.phrs.2019.104403"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm.4068"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1002\/jpen.1861"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471-4159.2008.05821.x"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.STR.0000217466.30085.82"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0891-5849(99)00152-5"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpregu.90825.2008"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1177\/0271678X19899577"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1136\/gutjnl-2020-323263"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12011-020-02300-x"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.1177\/0271678X16636890"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.4103\/bc.bc_32_18"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.1114-16.2016"},{"key":"e_1_2_9_40_1","doi-asserted-by":"publisher","DOI":"10.1161\/JAHA.115.002699"},{"key":"e_1_2_9_41_1","doi-asserted-by":"publisher","DOI":"10.21037\/atm-20-5622"},{"key":"e_1_2_9_42_1","doi-asserted-by":"publisher","DOI":"10.3389\/fneur.2019.00397"},{"key":"e_1_2_9_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jnutbio.2018.12.004"},{"key":"e_1_2_9_44_1","doi-asserted-by":"publisher","DOI":"10.1177\/0271678X19882788"},{"key":"e_1_2_9_45_1","doi-asserted-by":"publisher","DOI":"10.4161\/gmic.1.4.12778"},{"key":"e_1_2_9_46_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature12820"},{"key":"e_1_2_9_47_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471-4159.2009.06212.x"},{"key":"e_1_2_9_48_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.1359-19.2019"},{"key":"e_1_2_9_49_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00250272"},{"key":"e_1_2_9_50_1","doi-asserted-by":"publisher","DOI":"10.1038\/ismej.2011.59"},{"key":"e_1_2_9_51_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbi.2020.08.015"},{"key":"e_1_2_9_52_1","doi-asserted-by":"publisher","DOI":"10.3920\/BM2016.0184"},{"key":"e_1_2_9_53_1","doi-asserted-by":"publisher","DOI":"10.1128\/mSystems.00130-17"},{"key":"e_1_2_9_54_1","doi-asserted-by":"publisher","DOI":"10.1136\/jnnp-2017-317548"},{"key":"e_1_2_9_55_1","doi-asserted-by":"publisher","DOI":"10.1111\/cns.13613"},{"key":"e_1_2_9_56_1","doi-asserted-by":"publisher","DOI":"10.1097\/PSY.0000000000000493"},{"key":"e_1_2_9_57_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-020-14676-4"},{"key":"e_1_2_9_58_1","doi-asserted-by":"publisher","DOI":"10.1111\/cns.13451"},{"key":"e_1_2_9_59_1","doi-asserted-by":"publisher","DOI":"10.1177\/0271678X20918031"}],"container-title":["CNS Neuroscience &amp; Therapeutics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/cns.13736","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/cns.13736","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/cns.13736","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,28]],"date-time":"2023-08-28T04:15:07Z","timestamp":1693196107000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/cns.13736"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,9,30]]},"references-count":58,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2022,1]]}},"alternative-id":["10.1111\/cns.13736"],"URL":"https:\/\/doi.org\/10.1111\/cns.13736","archive":["Portico"],"relation":{},"ISSN":["1755-5930","1755-5949"],"issn-type":[{"value":"1755-5930","type":"print"},{"value":"1755-5949","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,9,30]]},"assertion":[{"value":"2021-06-28","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-09-18","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-09-30","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}