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Mice were intraperitoneally injected with 5\u00a0mg\/kg or 20\u2009mg\/kg apigenin daily for 28\u2009days after SCI. The Basso Mouse Scale (BMS) score, hematoxylin\u2013eosin staining, and immunohistochemical staining were used to assess the effect of apigenin on scar formation and motor function recovery. Western blotting and qRT\u2010PCR were used to detect the expression of fibrosis\u2010related parameters in spinal cord tissue homogenates. NIH\u20103 T3 cells and mouse primary spinal cord fibroblasts, \u03b1\u2010Smooth muscle actin (\u03b1\u2010SMA), collagen 1, and fibronectin were used to evaluate apigenin's effect in vitro. Western blotting and immunofluorescence techniques were used to study the effect of apigenin on TGF\u03b2\/SMADs signaling.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Apigenin inhibited fibrous scar formation in the mouse spinal cord and promoted the recovery of motor function. It reduced the expression of fibroblast\u2010related parameters and increased the content of nerve growth factor in vivo, decreasing myofibroblast activation and collagen fiber formation by inhibiting TGF\u03b2\u2010induced SMAD2\/3 phosphorylation and nuclear translocation in vitro.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Apigenin inhibits fibrous scar formation after SCI by decreasing fibrosis\u2010related factor expression through TGF\u03b2\/SMADs signaling.<\/jats:p><\/jats:sec>","DOI":"10.1111\/cns.13929","type":"journal-article","created":{"date-parts":[[2022,7,30]],"date-time":"2022-07-30T07:54:42Z","timestamp":1659167682000},"page":"1883-1894","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":47,"title":["Apigenin inhibits fibrous scar formation after acute spinal cord injury through TGF\u03b2\/SMADs signaling pathway"],"prefix":"10.1111","volume":"28","author":[{"given":"Zhengxin","family":"Jin","sequence":"first","affiliation":[{"name":"Central Hospital Affiliated to Shandong First Medical University Shandong First Medical University &amp; Shandong Academy of Medical Sciences  Jinan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lige","family":"Tian","sequence":"additional","affiliation":[{"name":"Tianjin Medical University  Tianjin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ying","family":"Zhang","sequence":"additional","affiliation":[{"name":"Central Hospital Affiliated to Shandong First Medical University Shandong First Medical University &amp; Shandong Academy of Medical Sciences  Jinan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaodi","family":"Zhang","sequence":"additional","affiliation":[{"name":"School of Clinical Medicine Weifang Medical University  Weifang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianning","family":"Kang","sequence":"additional","affiliation":[{"name":"Central Hospital Affiliated to Shandong First Medical University Shandong First Medical University &amp; Shandong Academy of Medical Sciences  Jinan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hui","family":"Dong","sequence":"additional","affiliation":[{"name":"Jinan Central Hospital, Cheeloo College of Medicine Shandong University  Jinan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nana","family":"Huang","sequence":"additional","affiliation":[{"name":"Central Hospital Affiliated to Shandong First Medical University Shandong First Medical University &amp; 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