{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,10]],"date-time":"2026-09-10T14:41:28Z","timestamp":1789051288677,"version":"build-2803163510"},"reference-count":44,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2022,11,23]],"date-time":"2022-11-23T00:00:00Z","timestamp":1669161600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81971095"],"award-info":[{"award-number":["81971095"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82001390"],"award-info":[{"award-number":["82001390"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["CNS Neurosci Ther"],"published-print":{"date-parts":[[2023,3]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Aims<\/jats:title>\n                    <jats:p>Remote ischemic pre\u2010conditioning (RIPC) protects against ischemia\/reperfusion (I\/R) injury. However, the mechanisms underlying this protection remain unclear. In the present study, we investigated the role of Janus\u2010activated kinase 2 (JAK2)\/signal transducer and activator of transcription 3 (STAT3) pathway and cell cycle arrest, and their relationship with neuronal apoptosis following RIPC.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>A rat cerebral I\/R injury model was induced by middle cerebral artery occlusion (MCAO), and AG490 was used to investigate the mechanisms of RIPC. p\u2010JAK2\u2010, p\u2010STAT3\u2010, cyclin D1\u2010, and cyclin\u2010dependent kinase 6 (CDK6) expression was assessed by Western blotting and immunofluorescence staining.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>RIPC reduced the infarct volume, improved neurological function, and increased neuronal survival. Furthermore, p\u2010JAK2 and p\u2010STAT3 were detected during the initial phase of reperfusion; the expression levels were significantly increased at 3 and 24\u2009h after reperfusion and were suppressed by RIPC. Additionally, the MCAO\u2010induced upregulation of the cell cycle regulators cyclin D1 and CDK6 was ameliorated by RIPC. Meanwhile, cyclin D1 and CDK6 were colocalized with p\u2010STAT3 in the ischemic brain.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>RIPC ameliorates the induction of the JAK2\/STAT3 pathway and cell cycle regulators cyclin D1 and CDK6 by MCAO, and this net inhibition of cell cycle re\u2010entry by RIPC is associated with downregulation of STAT3 phosphorylation.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/cns.14023","type":"journal-article","created":{"date-parts":[[2022,11,24]],"date-time":"2022-11-24T08:01:17Z","timestamp":1669276877000},"page":"866-877","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":27,"title":["Inhibition of the\n                    <scp>JAK2<\/scp>\n                    \/\n                    <scp>STAT3<\/scp>\n                    pathway and cell cycle re\u2010entry contribute to the protective effect of remote ischemic pre\u2010conditioning of rat hindlimbs on cerebral ischemia\/reperfusion injury"],"prefix":"10.1111","volume":"29","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2337-3216","authenticated-orcid":false,"given":"Yongmei","family":"Zhao","sequence":"first","affiliation":[{"name":"Institute of Cerebrovascular Disease Research Xuanwu Hospital of Capital Medical University  Beijing China"},{"name":"Beijing Geriatric Medical Research Center and Beijing Key Laboratory of Translational Medicine for Cerebrovascular Diseases  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mao","family":"Ding","sequence":"additional","affiliation":[{"name":"Institute of Cerebrovascular Disease Research Xuanwu Hospital of Capital Medical University  Beijing China"},{"name":"Beijing Geriatric Medical Research Center and Beijing Key Laboratory of Translational Medicine for Cerebrovascular Diseases  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Feng","family":"Yan","sequence":"additional","affiliation":[{"name":"Institute of Cerebrovascular Disease Research Xuanwu Hospital of Capital Medical University  Beijing China"},{"name":"Beijing Geriatric Medical Research Center and Beijing Key Laboratory of Translational Medicine for Cerebrovascular Diseases  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jie","family":"Yin","sequence":"additional","affiliation":[{"name":"Institute of Cerebrovascular Disease Research Xuanwu Hospital of Capital Medical University  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenjuan","family":"Shi","sequence":"additional","affiliation":[{"name":"Institute of Cerebrovascular Disease Research Xuanwu Hospital of Capital Medical University  Beijing China"},{"name":"Beijing Geriatric Medical Research Center and Beijing Key Laboratory of Translational Medicine for Cerebrovascular Diseases  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nan","family":"Yang","sequence":"additional","affiliation":[{"name":"Institute of Cerebrovascular Disease Research Xuanwu Hospital of Capital Medical University  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haiping","family":"Zhao","sequence":"additional","affiliation":[{"name":"Institute of Cerebrovascular Disease Research Xuanwu Hospital of Capital Medical University  Beijing China"},{"name":"Beijing Geriatric Medical Research Center and Beijing Key Laboratory of Translational Medicine for Cerebrovascular Diseases  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yalan","family":"Fang","sequence":"additional","affiliation":[{"name":"Institute of Cerebrovascular Disease Research Xuanwu Hospital of Capital Medical University  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuyou","family":"Huang","sequence":"additional","affiliation":[{"name":"Institute of Cerebrovascular Disease Research Xuanwu Hospital of Capital Medical University  Beijing China"},{"name":"Beijing Geriatric Medical Research Center and Beijing Key Laboratory of Translational Medicine for Cerebrovascular Diseases  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yangmin","family":"Zheng","sequence":"additional","affiliation":[{"name":"Institute of Cerebrovascular Disease Research Xuanwu Hospital of Capital Medical University  Beijing China"},{"name":"Beijing Geriatric Medical Research Center and Beijing Key Laboratory of Translational Medicine for Cerebrovascular Diseases  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xueqi","family":"Yang","sequence":"additional","affiliation":[{"name":"Institute of Cerebrovascular Disease Research Xuanwu Hospital of Capital Medical University  Beijing China"},{"name":"Beijing Geriatric Medical Research Center and Beijing Key Laboratory of Translational Medicine for Cerebrovascular Diseases  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei","family":"Li","sequence":"additional","affiliation":[{"name":"Institute of Cerebrovascular Disease Research Xuanwu Hospital of Capital Medical University  Beijing China"},{"name":"Beijing Geriatric Medical Research Center and Beijing Key Laboratory of Translational Medicine for Cerebrovascular Diseases  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0293-2744","authenticated-orcid":false,"given":"Xunming","family":"Ji","sequence":"additional","affiliation":[{"name":"Institute of Cerebrovascular Disease Research Xuanwu Hospital of Capital Medical University  Beijing China"},{"name":"Beijing Geriatric Medical Research Center and Beijing Key Laboratory of Translational Medicine for Cerebrovascular Diseases  Beijing China"},{"name":"Beijing Institute for Brain Disorders Capital Medical University  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2712-8974","authenticated-orcid":false,"given":"Yumin","family":"Luo","sequence":"additional","affiliation":[{"name":"Institute of Cerebrovascular Disease Research Xuanwu Hospital of Capital Medical University  Beijing China"},{"name":"Beijing Geriatric Medical Research Center and Beijing Key Laboratory of Translational Medicine for Cerebrovascular Diseases  Beijing China"},{"name":"Beijing Institute for Brain Disorders Capital Medical University  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,11,23]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1177\/0271678X221116192"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0735-1097(98)00559-2"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuroscience.2007.11.056"},{"issue":"8","key":"e_1_2_8_5_1","first-page":"583","article-title":"Protective effects of remote ischemic preconditioning in rat hindlimb on ischemia\u2010 reperfusion injury","volume":"7","author":"Zhang Y","year":"2012","journal-title":"Neural Regen Res"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI0215650"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1460-9568.1996.tb01556.x"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1098-1136(200005)30:3<253::AID-GLIA5>3.0.CO;2-O"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1006\/exnr.2001.7701"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.nbd.2006.08.007"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2009.05.014"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0952-7915(98)80165-9"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.brainres.2007.01.144"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0304-3940(01)01711-6"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471-4159.2006.04051.x"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M311768200"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pneurobio.2009.01.007"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.febslet.2005.07.028"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jns.2015.05.036"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.21-14-05045.2001"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.170144197"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1097\/MLG.0b013e31817fd3fa"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)81959-5"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2015.01.048"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.3892\/ijo.2015.3098"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1161\/STROKEAHA.113.004296"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.STR.27.9.1616"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.STR.20.1.84"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1038\/jcbfm.1990.47"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1166\/jnn.2020.16416"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1177\/0271678X211010351"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.1111\/imm.12255"},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.1177\/0271678X20952264"},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.1177\/0271678X20924077"},{"key":"e_1_2_8_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.lfs.2019.04.057"},{"key":"e_1_2_8_36_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2019.00620"},{"key":"e_1_2_8_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2017.09.091"},{"key":"e_1_2_8_38_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0501643102"},{"key":"e_1_2_8_39_1","doi-asserted-by":"publisher","DOI":"10.1038\/labinvest.2010.75"},{"key":"e_1_2_8_40_1","doi-asserted-by":"publisher","DOI":"10.1177\/0271678X19830791"},{"key":"e_1_2_8_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.expneurol.2021.113690"},{"key":"e_1_2_8_42_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1203947"},{"key":"e_1_2_8_43_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12035-020-02016-y"},{"key":"e_1_2_8_44_1","doi-asserted-by":"publisher","DOI":"10.1097\/00004647-199708000-00003"},{"key":"e_1_2_8_45_1","doi-asserted-by":"publisher","DOI":"10.2174\/1871527311312030008"}],"container-title":["CNS Neuroscience &amp; Therapeutics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/cns.14023","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/cns.14023","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/cns.14023","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,17]],"date-time":"2023-08-17T23:45:07Z","timestamp":1692315907000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/cns.14023"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,11,23]]},"references-count":44,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2023,3]]}},"alternative-id":["10.1111\/cns.14023"],"URL":"https:\/\/doi.org\/10.1111\/cns.14023","archive":["Portico"],"relation":{},"ISSN":["1755-5930","1755-5949"],"issn-type":[{"value":"1755-5930","type":"print"},{"value":"1755-5949","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,11,23]]},"assertion":[{"value":"2022-06-04","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-09-21","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-11-23","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}