{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T20:22:41Z","timestamp":1790108561404,"version":"4.0.1"},"reference-count":61,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2023,2,8]],"date-time":"2023-02-08T00:00:00Z","timestamp":1675814400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81870905"],"award-info":[{"award-number":["81870905"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["U20A20358"],"award-info":[{"award-number":["U20A20358"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["CNS Neurosci Ther"],"published-print":{"date-parts":[[2023,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Aims<\/jats:title><jats:p>To investigate the role of exosomal miR\u2010128\u20103p in promoting fibrinogen\u2010mediated inhibition of oligodendrocyte progenitor cell (OPC) differentiation and the therapeutic potential of exosomal miR\u2010128\u20103p in cerebral ischemia.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Mouse models of middle cerebral artery occlusion (MCAO) were established as described previously. MCAO was treated with fibrinogen and exosomes by stereotactically injecting into the left stratum. Mouse cortical OPCs were used for mRNA and miRNA sequencing analysis. Exosomes were isolated from neural stem cells (NSCs) of mice.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Fibrinogen deposition suppressed remyelination after MCAO and inhibited OPC differentiation by activating ACVR1, the bone morphogenetic protein (BMP) signaling type I receptor. In vitro, miR\u2010sequencing and verification studies revealed that miR\u2010128\u20103p is associated with BMP signaling mediated by ACVR1. Additionally, transfer of NSC\u2010derived exosomal miR\u2010128\u20103p to OPCs significantly increased myelin basic protein expression and inhibited BMP signaling. Furthermore, NSC\u2010derived exosomal miR\u2010128\u20103p protected against fibrinogen\u2010induced demyelination related to BMP signaling, reduced the infarct volume, and improved neurological function after MCAO.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Fibrinogen deposition inhibits remyelination after ischemic damage and NSC\u2010derived exosomal miR\u2010128\u20103p promotes OPC differentiation into OLs by suppressing BMP signaling, indicating that NSC\u2010derived exosomal miR\u2010128\u20103p represents a potential therapeutic target for ischemic stroke.<\/jats:p><\/jats:sec>","DOI":"10.1111\/cns.14113","type":"journal-article","created":{"date-parts":[[2023,2,9]],"date-time":"2023-02-09T08:02:21Z","timestamp":1675929741000},"page":"1405-1422","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":30,"title":["Exosomal <scp>miR<\/scp>\u2010128\u20103p reversed fibrinogen\u2010mediated inhibition of oligodendrocyte progenitor cell differentiation and remyelination after cerebral ischemia"],"prefix":"10.1111","volume":"29","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0226-968X","authenticated-orcid":false,"given":"Huiqing","family":"Hou","sequence":"first","affiliation":[{"name":"Department of Neurology, Beijing Tiantan Hospital Capital Medical University  Beijing China"},{"name":"Department of Neurology the Second Hospital of Hebei Medical University  Shijiazhuang China"},{"name":"China National Clinical Research Center for Neurological Diseases  Beijing China"},{"name":"Research Unit of Artificial Intelligence in Cerebrovascular Disease, Chinese Academy of Medical Sciences  Beijing China"},{"name":"Center for Excellence in Brain Science and Intelligence Technology, Chinese Academy of Sciences  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yafei","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Neurology the Second Hospital of Hebei Medical University  Shijiazhuang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lan","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Neurology the Second Hospital of Hebei Medical University  Shijiazhuang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yongjun","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Neurology, Beijing Tiantan Hospital Capital Medical University  Beijing China"},{"name":"China National Clinical Research Center for Neurological Diseases  Beijing China"},{"name":"Research Unit of Artificial Intelligence in Cerebrovascular Disease, Chinese Academy of Medical Sciences  Beijing China"},{"name":"Center for Excellence in Brain Science and Intelligence Technology, Chinese Academy of Sciences  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,2,8]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00281-011-0290-8"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.atherosclerosis.2021.10.002"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.STR.0000226991.27540.f2"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-020-14466-y"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.0137-10.2010"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2017.10.008"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/hep.30607"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1136\/jitc-2020-001293"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.5966\/sctm.2015-0078"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1177\/0271678X17708917"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1161\/STROKEAHA.116.015204"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12975-017-0599-2"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1161\/STROKEAHA.117.020353"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12035-016-9851-0"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1161\/STROKEAHA.117.018292"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41582-018-0126-4"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.119.315886"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1476-5381.2010.00872.x"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13287-020-02091-x"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.expneurol.2021.113611"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0896-6273(02)00617-7"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41419-019-2206-9"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jare.2020.05.017"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2012.01.020"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.freeradbiomed.2020.11.032"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1177\/0271678X20941393"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1177\/0271678X19830791"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcell.2021.601600"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12974-021-02181-4"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.1177\/1535370219895491"},{"key":"e_1_2_10_32_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10571-019-00770-9"},{"key":"e_1_2_10_33_1","doi-asserted-by":"publisher","DOI":"10.1093\/brain\/awy082"},{"key":"e_1_2_10_34_1","doi-asserted-by":"publisher","DOI":"10.1038\/nn.3676"},{"key":"e_1_2_10_35_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13046-019-1051-0"},{"key":"e_1_2_10_36_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature07086"},{"key":"e_1_2_10_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.stem.2015.11.019"},{"key":"e_1_2_10_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jns.2005.05.006"},{"key":"e_1_2_10_39_1","doi-asserted-by":"publisher","DOI":"10.1160\/TH07-08-0484"},{"key":"e_1_2_10_40_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrn.2018.13"},{"key":"e_1_2_10_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2017.07.030"},{"key":"e_1_2_10_42_1","doi-asserted-by":"publisher","DOI":"10.2174\/092986707782360015"},{"key":"e_1_2_10_43_1","doi-asserted-by":"publisher","DOI":"10.3389\/fncel.2015.00354"},{"key":"e_1_2_10_44_1","doi-asserted-by":"publisher","DOI":"10.1002\/ana.24997"},{"key":"e_1_2_10_45_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0303859101"},{"key":"e_1_2_10_46_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbt.22752"},{"key":"e_1_2_10_47_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.omtn.2019.02.002"},{"key":"e_1_2_10_48_1","doi-asserted-by":"publisher","DOI":"10.3389\/fncel.2014.00377"},{"key":"e_1_2_10_49_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tins.2018.03.006"},{"key":"e_1_2_10_50_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrd3978"},{"key":"e_1_2_10_51_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12017-019-08540-y"},{"key":"e_1_2_10_52_1","doi-asserted-by":"publisher","DOI":"10.1038\/jcbfm.2013.152"},{"key":"e_1_2_10_53_1","doi-asserted-by":"publisher","DOI":"10.1080\/20013078.2017.1286095"},{"key":"e_1_2_10_54_1","doi-asserted-by":"publisher","DOI":"10.1177\/0271678X20950489"},{"key":"e_1_2_10_55_1","doi-asserted-by":"publisher","DOI":"10.1002\/stem.1409"},{"key":"e_1_2_10_56_1","doi-asserted-by":"publisher","DOI":"10.1002\/adma.201807591"},{"key":"e_1_2_10_57_1","doi-asserted-by":"publisher","DOI":"10.1634\/stemcells.2007-0284"},{"key":"e_1_2_10_58_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.01273"},{"key":"e_1_2_10_59_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.omtn.2017.04.010"},{"key":"e_1_2_10_60_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(16)30513-X"},{"key":"e_1_2_10_61_1","doi-asserted-by":"publisher","DOI":"10.1177\/1545968309335978"},{"key":"e_1_2_10_62_1","doi-asserted-by":"publisher","DOI":"10.3402\/jev.v4.26316"}],"container-title":["CNS Neuroscience &amp; Therapeutics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/cns.14113","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/cns.14113","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/cns.14113","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,21]],"date-time":"2023-08-21T14:36:19Z","timestamp":1692628579000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/cns.14113"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,2,8]]},"references-count":61,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2023,5]]}},"alternative-id":["10.1111\/cns.14113"],"URL":"https:\/\/doi.org\/10.1111\/cns.14113","archive":["Portico"],"relation":{},"ISSN":["1755-5930","1755-5949"],"issn-type":[{"value":"1755-5930","type":"print"},{"value":"1755-5949","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,2,8]]},"assertion":[{"value":"2022-11-04","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-01-20","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-02-08","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}