{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T22:49:49Z","timestamp":1792622989642,"version":"4.5.0"},"reference-count":48,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2023,6,2]],"date-time":"2023-06-02T00:00:00Z","timestamp":1685664000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["62101164"],"award-info":[{"award-number":["62101164"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["62172131"],"award-info":[{"award-number":["62172131"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["CNS Neurosci Ther"],"published-print":{"date-parts":[[2023,10]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Aims<\/jats:title>\n                    <jats:p>Complex cellular communications between glial cells and neurons are critical for brain normal function and disorders, and single\u2010cell level RNA\u2010sequencing datasets display more advantages for analyzing cell communications. Therefore, it is necessary to systematically explore brain cell communications when considering factors such as sex and brain region.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>We extracted a total of 1,039,459 cells derived from 28 brain single\u2010cell RNA\u2010sequencing (scRNA\u2010seq) or single\u2010nucleus RNA\u2010sequencing (snRNA\u2010seq) datasets from the GEO database, including 12 human and 16 mouse datasets. These datasets were further divided into 71 new sub\u2010datasets when considering disease, sex, and region conditions. In the meanwhile, we integrated four methods to evaluate ligand\u2013receptor interaction score among six major brain cell types (microglia, neuron, astrocyte, oligodendrocyte, OPC, and endothelial cell).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>For Alzheimer's disease (AD), disease\u2010specific ligand\u2013receptor pairs when compared with normal sub\u2010datasets, such as SEMA4A\u2010NRP1, were identified. Furthermore, we explored the sex\u2010 and region\u2010specific cell communications and identified that WNT5A\u2010ROR1 among microglia cells displayed close communications in male, and SPP1\u2010ITGAV displayed close communications in the meninges region from microglia to neurons. Furthermore, based on the AD\u2010specific cell communications, we constructed a model for AD early prediction and confirmed the predictive performance using multiple independent datasets. Finally, we developed an online platform for researchers to explore brain condition\u2010specific cell communications.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>This research provided a comprehensive study to explore brain cell communications, which could reveal novel biological mechanisms involved in normal brain function and neurodegenerative diseases such as AD.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/cns.14280","type":"journal-article","created":{"date-parts":[[2023,6,3]],"date-time":"2023-06-03T00:34:26Z","timestamp":1685752466000},"page":"2775-2786","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":38,"title":["Comprehensive analyses of brain cell communications based on multiple scRNA\u2010seq and snRNA\u2010seq datasets for revealing novel mechanism in neurodegenerative 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