{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T19:26:31Z","timestamp":1790105191424,"version":"4.0.1"},"reference-count":42,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2023,6,22]],"date-time":"2023-06-22T00:00:00Z","timestamp":1687392000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["CNS Neurosci Ther"],"published-print":{"date-parts":[[2023,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Aims<\/jats:title><jats:p>MicroRNAs (miRs) are involved in endogenous neurogenesis, enhancing of which has been regarded as a potential therapeutic strategy for ischemic stroke treatment; however, whether miR\u2010199a\u20105p mediates postischemic neurogenesis remains unclear. This study aims to investigate the proneurogenesis effects of miR\u2010199a\u20105p and its possible mechanism after ischemic stroke.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Neural stem cells (NSCs) were transfected using Lipofectamine 3000 reagent, and the differentiation of NSCs was evaluated by immunofluorescence and Western blotting. Dual\u2010luciferase reporter assay was performed to verify the target gene of miR\u2010199a\u20105p. MiR\u2010199a\u20105p agomir\/antagomir were injected intracerebroventricularly. The sensorimotor functions were evaluated by neurobehavioral tests, infarct volume was measured by toluidine blue staining, neurogenesis was detected by immunofluorescence assay, and the protein levels of neuronal nuclei (NeuN), glial fibrillary acidic protein (GFAP), caveolin\u20101 (Cav\u20101), vascular endothelial growth factor (VEGF), and brain\u2010derived neurotrophic factor (BDNF) were measured by Western blotting.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>MiR\u2010199a\u20105p mimic enhanced neuronal differentiation and inhibited astrocyte differentiation of NSCs, while a miR\u2010199a\u20105p inhibitor induced the opposite effects, which can be reversed by Cav\u20101 siRNA. Cav\u20101 was through the dual\u2010luciferase reporter assay confirmed as a target gene of miR\u2010199a\u20105p. miR\u2010199a\u20105p agomir in rat stroke models manifested multiple benefits, such as improving neurological deficits, reducing infarct volume, promoting neurogenesis, inhibiting Cav\u20101, and increasing VEGF and BDNF, which was reversed by the miR\u2010199a\u20105p antagomir.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>MiR\u2010199a\u20105p may target and inhibit Cav\u20101 to enhance neurogenesis and thus promote functional recovery after cerebral ischemia. These findings indicate that miR\u2010199a\u20105p is a promising target for the treatment of ischemic stroke.<\/jats:p><\/jats:sec>","DOI":"10.1111\/cns.14323","type":"journal-article","created":{"date-parts":[[2023,6,24]],"date-time":"2023-06-24T23:03:13Z","timestamp":1687647793000},"page":"3967-3979","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":21,"title":["<scp>MiR<\/scp>\u2010199a\u20105p enhances neuronal differentiation of neural stem cells and promotes neurogenesis by targeting Cav\u20101 after cerebral ischemia"],"prefix":"10.1111","volume":"29","author":[{"given":"Hua\u2010Qian","family":"Jin","sequence":"first","affiliation":[{"name":"Department of Physiology Zhejiang Chinese Medical University  Hangzhou 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