{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,17]],"date-time":"2026-07-17T12:29:01Z","timestamp":1784291341308,"version":"3.55.0"},"reference-count":35,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2023,10,30]],"date-time":"2023-10-30T00:00:00Z","timestamp":1698624000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["CNS Neurosci Ther"],"published-print":{"date-parts":[[2024,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Aim<\/jats:title><jats:p>As the main type of stroke, the incidence of cerebral venous thrombosis (CVT) has been rising. However, the comprehensive mechanisms behind it remain unclear. Thus, the multi\u2010omics study is required to investigate the mechanism after CVT and elucidate the characteristic pathology of venous stroke and arterial stroke.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Adult rats were subjected to CVT and MCAO models. Whole\u2010transcriptome sequencing (RNA\u2010seq) and untargeted metabolomics analysis were performed to construct the transcriptome and metabolism profiles of rat brains after CVT and also MCAO. The difference analysis, functional annotation, and enrichment analysis were also performed.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Through RNA\u2010seq analysis, differentially expressed genes (DEGs) were screened. 174 CVT specific genes including <jats:italic>Il1a<\/jats:italic>, <jats:italic>Ccl9<\/jats:italic>, <jats:italic>Cxxl6<\/jats:italic>, <jats:italic>Tnfrsf14<\/jats:italic>, etc., were detected. The hemoglobin genes, including both <jats:italic>Hba<\/jats:italic> and <jats:italic>Hbb<\/jats:italic>, were significantly downregulated after CVT, compared both to the MCAO and Sham groups. Metabolism analysis showed that CVT had higher heterogeneity of metabolism compared to MCAO. Metabolites including N\u2010stearoyltyrosine, 5\u2010methoxy\u20103\u2010indoleaceate, Afegostat, pipecolic acid, etc. were specially regulated in CVT. Through the immune infiltration analysis, it was found that CVT had a higher immune response, with the abundance of certain types of immune cells increased, especially T helper cells. It was important to find the prevalence of the activation of inflammatory chemokine, cytokine, NOD\u2010like pathway, and neutrophil extracellular trap.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>We explored and analyzed the gene expression and metabolomic characteristics of CVT, revealed the specific inflammatory reaction mechanism of CVT and found the markers in transcriptome and metabolism levels. It points out the direction for CVT early diagnosis and treatment.<\/jats:p><\/jats:sec>","DOI":"10.1111\/cns.14494","type":"journal-article","created":{"date-parts":[[2023,10,30]],"date-time":"2023-10-30T11:56:46Z","timestamp":1698667006000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Expression and metabolism profiles of <scp>CVT<\/scp> associated with inflammatory responses and oxygen carrier ability in the brain"],"prefix":"10.1111","volume":"30","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1416-1601","authenticated-orcid":false,"given":"Ling","family":"Kui","sequence":"first","affiliation":[{"name":"Shenzhen Qianhai Shekou Free Trade Zone Hospital  Shenzhen China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yinming","family":"Jiao","sequence":"additional","affiliation":[{"name":"Shenzhen Qianhai Shekou Free Trade Zone Hospital  Shenzhen 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