{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T15:07:46Z","timestamp":1791299266534,"version":"4.1.0"},"reference-count":57,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2023,10,31]],"date-time":"2023-10-31T00:00:00Z","timestamp":1698710400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004731","name":"Natural Science Foundation of Zhejiang Province","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004731","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["CNS Neurosci Ther"],"published-print":{"date-parts":[[2024,4]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Aims<\/jats:title>\n                    <jats:p>Peripheral immune cells infiltrating into the brain trigger neuroinflammation after an ischemic stroke. Partial immune cells reprogram their function for neural repair. Which immune cells promote ischemic brain recovery needs further identification.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      We performed single\u2010cell transcriptomic profiling of CD45\n                      <jats:sup>high<\/jats:sup>\n                      immune cells isolated from the ischemic hemisphere at subacute (5\u2009days) and chronic (14\u2009days) stages after ischemic stroke.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      A subset of phagocytic macrophages was associated with neuron projection regeneration and tissue remodeling. We also identified a unique type of T cells with highly expressed macrophage markers, including\n                      <jats:italic>C1q<\/jats:italic>\n                      ,\n                      <jats:italic>Apoe<\/jats:italic>\n                      ,\n                      <jats:italic>Hexb<\/jats:italic>\n                      , and\n                      <jats:italic>Fcer1g<\/jats:italic>\n                      , which showed high abilities in tissue remodeling, myelination regulation, wound healing, and anti\u2010neuroinflammation. Moreover, natural killer cells decreased cytotoxicity and increased energy and metabolic function in the chronic stage after ischemic stroke. Two subgroups of neutrophils upregulated CCL signals to recruit peripheral immune cells and released CXCL2 to keep self\u2010recruiting at the chronic stage.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>We identified subsets of peripheral immune cells that may provide potential therapeutic targets for promoting poststroke recovery.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/cns.14518","type":"journal-article","created":{"date-parts":[[2023,10,31]],"date-time":"2023-10-31T06:39:22Z","timestamp":1698734362000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Novel subsets of peripheral immune cells associated with promoting stroke recovery in mice"],"prefix":"10.1111","volume":"30","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0439-859X","authenticated-orcid":false,"given":"Yichen","family":"Gu","sequence":"first","affiliation":[{"name":"Department of Neurosurgery Second Affiliated Hospital School of Medicine Zhejiang University  Hangzhou Zhejiang China"},{"name":"Clinical Research Center for Neurological Diseases of Zhejiang Province  Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaotao","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Neurosurgery Second Affiliated Hospital School of Medicine Zhejiang University  Hangzhou Zhejiang China"},{"name":"Clinical Research Center for Neurological Diseases of Zhejiang Province  Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huaming","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Neurosurgery Second Affiliated Hospital School of Medicine Zhejiang University  Hangzhou Zhejiang China"},{"name":"Clinical Research Center for Neurological Diseases of Zhejiang Province  Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rui","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Neurosurgery Second Affiliated Hospital School of Medicine Zhejiang University  Hangzhou Zhejiang China"},{"name":"Clinical Research Center for Neurological Diseases of Zhejiang Province  Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chenghao","family":"Jin","sequence":"additional","affiliation":[{"name":"Department of Neurosurgery Second Affiliated Hospital School of Medicine Zhejiang University  Hangzhou Zhejiang China"},{"name":"Clinical Research Center for Neurological Diseases of Zhejiang Province  Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junjie","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Neurosurgery Second Affiliated Hospital School of Medicine Zhejiang University  Hangzhou Zhejiang China"},{"name":"Clinical Research Center for Neurological Diseases of Zhejiang Province  Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ziyang","family":"Jin","sequence":"additional","affiliation":[{"name":"Department of Neurosurgery Second Affiliated Hospital School of Medicine Zhejiang University  Hangzhou Zhejiang China"},{"name":"Clinical Research Center for Neurological Diseases of Zhejiang Province  Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianan","family":"Lu","sequence":"additional","affiliation":[{"name":"Department of Neurosurgery Second Affiliated Hospital School of Medicine Zhejiang University  Hangzhou Zhejiang China"},{"name":"Clinical Research Center for Neurological Diseases of Zhejiang Province  Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chenhan","family":"Ling","sequence":"additional","affiliation":[{"name":"Department of Neurosurgery Second Affiliated Hospital School of Medicine Zhejiang University  Hangzhou Zhejiang China"},{"name":"Clinical Research Center for Neurological Diseases of Zhejiang Province  Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fangjie","family":"Shao","sequence":"additional","affiliation":[{"name":"Department of Neurosurgery Second Affiliated Hospital School of Medicine Zhejiang University  Hangzhou Zhejiang China"},{"name":"Clinical Research Center for Neurological Diseases of Zhejiang Province  Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3184-1502","authenticated-orcid":false,"given":"Jianmin","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Neurosurgery Second Affiliated Hospital School of Medicine Zhejiang University  Hangzhou Zhejiang China"},{"name":"Clinical Research Center for Neurological Diseases of Zhejiang Province  Hangzhou China"},{"name":"Brain Research Institute Zhejiang University  Hangzhou Zhejiang China"},{"name":"Collaborative Innovation Center for Brain Science Zhejiang University  Hangzhou Zhejiang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ligen","family":"Shi","sequence":"additional","affiliation":[{"name":"Department of Neurosurgery Second Affiliated Hospital School of Medicine Zhejiang University  Hangzhou Zhejiang China"},{"name":"Clinical Research Center for Neurological Diseases of Zhejiang Province  Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,10,31]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1474-4422(21)00252-0"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pbio.3000330"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41583-023-00690-0"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41583-020-00396-7"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1474-4422(19)30078-X"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrn3710"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms11499"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.2435-08.2008"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/cns.13620"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm.4312"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12974-016-0750-0"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1177\/0271678X19888630"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.1867-14.2014"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCULATIONAHA.105.593046"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm.1927"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI157678"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-018-0824-5"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12974-022-02447-5"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1177\/0271678X211026770"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcell.2021.624711"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2021.04.022"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2019.05.031"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btv300"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-021-21246-9"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1038\/nbt.2859"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-019-0969-x"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.4463"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.1161\/STROKEAHA.108.534503"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.3389\/fnhum.2014.00381"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00401-014-1381-0"},{"key":"e_1_2_10_32_1","doi-asserted-by":"publisher","DOI":"10.1212\/NXI.0000000000000402"},{"key":"e_1_2_10_33_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2021.674739"},{"key":"e_1_2_10_34_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2021.656097"},{"key":"e_1_2_10_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2017.02.015"},{"key":"e_1_2_10_36_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41375-021-01393-0"},{"key":"e_1_2_10_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cytogfr.2018.08.001"},{"key":"e_1_2_10_38_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-021-22973-9"},{"key":"e_1_2_10_39_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41590-021-00968-4"},{"key":"e_1_2_10_40_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2013-06-510909"},{"key":"e_1_2_10_41_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCULATIONAHA.120.050186"},{"key":"e_1_2_10_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molimm.2010.03.003"},{"key":"e_1_2_10_43_1","doi-asserted-by":"publisher","DOI":"10.1124\/jpet.105.089607"},{"key":"e_1_2_10_44_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-019-10970-y"},{"key":"e_1_2_10_45_1","doi-asserted-by":"publisher","DOI":"10.1038\/ni.3486"},{"key":"e_1_2_10_46_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.2018886077"},{"key":"e_1_2_10_47_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2005-10-009191"},{"key":"e_1_2_10_48_1","doi-asserted-by":"publisher","DOI":"10.1038\/nn.4338"},{"key":"e_1_2_10_49_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00281-022-00975-z"},{"key":"e_1_2_10_50_1","doi-asserted-by":"publisher","DOI":"10.1111\/cns.13611"},{"key":"e_1_2_10_51_1","doi-asserted-by":"publisher","DOI":"10.3390\/cells11091490"},{"key":"e_1_2_10_52_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm.4068"},{"key":"e_1_2_10_53_1","doi-asserted-by":"publisher","DOI":"10.1111\/neup.12387"},{"key":"e_1_2_10_54_1","doi-asserted-by":"publisher","DOI":"10.3389\/fncel.2020.00206"},{"key":"e_1_2_10_55_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20202411"},{"key":"e_1_2_10_56_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-020-16191-y"},{"key":"e_1_2_10_57_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2018.09.018"},{"key":"e_1_2_10_58_1","doi-asserted-by":"publisher","DOI":"10.1111\/cns.13899"}],"container-title":["CNS Neuroscience &amp; Therapeutics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/cns.14518","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,5,28]],"date-time":"2024-05-28T23:59:45Z","timestamp":1716940785000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/cns.14518"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,10,31]]},"references-count":57,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2024,4]]}},"alternative-id":["10.1111\/cns.14518"],"URL":"https:\/\/doi.org\/10.1111\/cns.14518","archive":["Portico"],"relation":{},"ISSN":["1755-5930","1755-5949"],"issn-type":[{"value":"1755-5930","type":"print"},{"value":"1755-5949","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,10,31]]},"assertion":[{"value":"2023-08-20","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-10-18","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-10-31","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e14518"}}