{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,30]],"date-time":"2026-10-30T03:20:41Z","timestamp":1793330441982,"version":"4.5.0"},"reference-count":45,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2024,3,3]],"date-time":"2024-03-03T00:00:00Z","timestamp":1709424000000},"content-version":"vor","delay-in-days":2,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100002858","name":"China Postdoctoral Science Foundation","doi-asserted-by":"publisher","award":["2020M673649"],"award-info":[{"award-number":["2020M673649"]}],"id":[{"id":"10.13039\/501100002858","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100009156","name":"Nantong Municipal Science and Technology Bureau","doi-asserted-by":"publisher","award":["MS12021020"],"award-info":[{"award-number":["MS12021020"]}],"id":[{"id":"10.13039\/501100009156","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100009156","name":"Nantong Municipal Science and Technology Bureau","doi-asserted-by":"publisher","award":["MS22021010"],"award-info":[{"award-number":["MS22021010"]}],"id":[{"id":"10.13039\/501100009156","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81873924"],"award-info":[{"award-number":["81873924"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82171190"],"award-info":[{"award-number":["82171190"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["CNS Neurosci Ther"],"published-print":{"date-parts":[[2024,3]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Aim<\/jats:title>\n                    <jats:p>This study aims to elucidate the cellular dynamics and pathophysiology of white matter hemorrhage (WMH) in intracerebral hemorrhage (ICH).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Using varying doses of collagenase IV, a consistent rat ICH model characterized by pronounced WMH was established. Verification was achieved through behavioral assays, hematoma volume, and histological evaluations. Single\u2010cell suspensions from the hemorrhaged region of the ipsilateral striatum on day three post\u2010ICH were profiled using single\u2010cell RNA sequencing (scRNA\u2010seq). Gene Ontology (GO) and gene set variation analysis (GSVA) further interpreted the differentially expressed genes (DEGs).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Following WMH induction, there was a notable increase in the percentage of myeloid cells and oligodendrocyte precursor cells (OPCs), alongside a reduction in the percentage of neurons, microglia, and oligodendrocytes (OLGs). Post\u2010ICH WMH showed homeostatic microglia transitioning into pro\u2010, anti\u2010inflammatory, and proliferative states, influencing lipid metabolic pathways. Myeloid cells amplified chemokine expression, linked with ferroptosis pathways. Macrophages exhibited M1 and M2 phenotypes, and post\u2010WMH, macrophages displayed a predominance of M2 phenotypes, characterized by their anti\u2010inflammatory properties. A surge in OPC proliferation aligned with enhanced ribosomal signaling, suggesting potential reparative responses post\u2010WMH.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>The study offers valuable insights into WMH's complex pathophysiology following ICH, highlighting the significance and utility of scRNA\u2010seq in understanding the cellular dynamics and contributing to future cerebrovascular research.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/cns.14652","type":"journal-article","created":{"date-parts":[[2024,3,3]],"date-time":"2024-03-03T21:14:30Z","timestamp":1709500470000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":41,"title":["Unraveling the complex pathophysiology of white matter hemorrhage in intracerebral stroke: A single\u2010cell RNA sequencing 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