{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,28]],"date-time":"2026-08-28T16:14:34Z","timestamp":1787933674671,"version":"build-2784847793"},"reference-count":25,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2024,3,15]],"date-time":"2024-03-15T00:00:00Z","timestamp":1710460800000},"content-version":"vor","delay-in-days":14,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004826","name":"Natural Science Foundation of Beijing Municipality","doi-asserted-by":"publisher","award":["7222083"],"award-info":[{"award-number":["7222083"]}],"id":[{"id":"10.13039\/501100004826","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81801149"],"award-info":[{"award-number":["81801149"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82171301"],"award-info":[{"award-number":["82171301"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["CNS Neurosci Ther"],"published-print":{"date-parts":[[2024,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Aim<\/jats:title><jats:p>To explore the neuroprotective effects of ARA290 and the role of \u03b2\u2010common receptor (\u03b2CR) in a mouse model of middle cerebral artery occlusion (MCAO).<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>This study included male C57BL\/6J mice that underwent MCAO and reperfusion. The neuroprotective effect of ARA290 on MCAO\u2010induced brain injury was investigated using neurological function tests (Longa and modified neurological severity score). Cerebral infarction was examined by 2, 3, 5\u2010triphenyl tetrazolium chloride staining, neuronal apoptosis was assessed by immunofluorescence staining, blood parameters were measured using a flow cytometry\u2010based automated hematology analyzer, liquid chromatography with tandem mass spectrometry was used to identify the serum metabolomics signature, inflammatory cytokines and liver index were detected by commercially available kits, and the protein levels of the erythropoietin (EPO) receptor and \u03b2CR were measured by western blot.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>ARA290 exerted a qualitatively similar neuroprotective effect after MCAO as EPO. ARA290 significantly reduced neuronal apoptosis and the level of inflammatory cytokines in the brain tissue. However, ARA290's neuroprotective effect was significantly suppressed following the injection of siRNA against \u03b2CR.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>ARA290 provided a neuroprotective effect via \u03b2CR in cerebral ischemic mice without causing erythropoiesis. This study provides novel insights into the role of ARA290 in ischemic stroke intervention.<\/jats:p><\/jats:sec>","DOI":"10.1111\/cns.14676","type":"journal-article","created":{"date-parts":[[2024,3,16]],"date-time":"2024-03-16T06:45:00Z","timestamp":1710571500000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Erythropoietin\u2010derived peptide ARA290 mediates brain tissue protection through the \u03b2\u2010common receptor in mice with cerebral ischemic stroke"],"prefix":"10.1111","volume":"30","author":[{"given":"Rong\u2010Liang","family":"Wang","sequence":"first","affiliation":[{"name":"Institute of Cerebrovascular Diseases Research and Department of Neurology Xuanwu Hospital of Capital Medical University  Beijing China"},{"name":"Center of Stroke Beijing Institute for Brain Disorders  Beijing China"},{"name":"Beijing Key Laboratory of Translational Medicine for Cerebrovascular Diseases  Beijing 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Cerebrovascular Diseases Research and Department of Neurology Xuanwu Hospital of Capital Medical University  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Feng","family":"Yan","sequence":"additional","affiliation":[{"name":"Institute of Cerebrovascular Diseases Research and Department of Neurology Xuanwu Hospital of Capital Medical University  Beijing China"},{"name":"Center of Stroke Beijing Institute for Brain Disorders  Beijing China"},{"name":"Beijing Key Laboratory of Translational Medicine for Cerebrovascular Diseases  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yang\u2010Min","family":"Zheng","sequence":"additional","affiliation":[{"name":"Institute of Cerebrovascular Diseases Research and Department of Neurology Xuanwu Hospital of Capital Medical University  Beijing China"},{"name":"Center of Stroke Beijing Institute for Brain Disorders  Beijing China"},{"name":"Beijing Key Laboratory of Translational Medicine 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